Restriction map for pF1KB6334
##################################
# NO CUT ENZYMES (  174 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AclI        
AcsI        AcyI        AfaI        AflII       AgeI        
AhdI        Alw44I      AlwNI       AocI        Aor51HI     
ApaI        ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspHI       
AspI        AvaI        AviII       AvrII       BalI        
BamHI       BanI        BanII       BbeI        BbsI        
BbvCI       BcgI        BciVI       BclI        BfrI        
BglI        BlnI        BlpI        BmyI        Bpu1102I    
BpuAI       BsaHI       BsaI        BsiEI       BsiHKAI     
BsiWI       BsmAI       BsmBI       BsmI        BsoBI       
Bsp1286I    Bsp1407I    BspDI       BspHI       BspMI       
BsrBI       BsrDI       BsrFI       BsrGI       BssHII      
Bst1107I    BstAPI      BstBI       BstEII      BstNI       
BstPI       BstUI       BstZ17I     Bsu36I      CelII       
CfoI        Cfr10I      ClaI        CpoI        DraI        
DraII       DrdI        DsaI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoRII      EcoRV       
EcoT22I     FauI        FbaI        FseI        FspI        
HaeII       HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HindIII     HpaI        KasI        
KpnI        KspI        MaeIII      MfeI        MluI        
MluNI       MscI        MunI        MvaI        MvnI        
NaeI        NarI        NcoI        NdeI        NgoMIV      
NheI        NlaIV       NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmeI        PpuMI       PshAI       PshBI       
Psp1406I    PstI        PvuI        RcaI        RsaI        
RsrII       SacI        SacII       SalI        SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
Tsp45I      Tth111I     Van91I      XbaI        XcmI        
XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   33 enzymes) #
###################################

AccIII        272  TCCGGA               T^CCGGA
AlwI          539  GGATC                GGATC(4/5)
BbrPI         724  CACGTG               CAC^GTG
BglII         143  AGATCT               A^GATCT
BsaAI         724  CACGTG               [CT]AC^GT[GA]
BsaJI         218  CCAAGG               C^C[ACGT][ACGT]GG
BsaWI         272  TCCGGA               [AT]^CCGG[AT]
BseAI         272  TCCGGA               T^CCGGA
BsgI          194  CTGCAC               GTGCAG(16/14)
BsiYI         768  CCAGAGTCAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          768  CCAGAGTCAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI         585  GGGAC                GGGAC(10/14)
BspEI         272  TCCGGA               T^CCGGA
BsrI          466  ACTGG                ACTGG(1/-1)
BssSI         737  CACGAG               CACGAG(-5/-1)
BstXI         656  CCATGCTGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         143  AGATCT               [GA]^GATC[CT]
Cac8I         564  GCAAGC               GC[ACGT]^[ACGT]GC
DraIII        712  CACACAGTG            CAC[ACGT][ACGT][ACGT]^GTG
EaeI          521  CGGCCA               [CT]^GGCC[GA]
EarI          285  GAAGAG               CTCTTC(1/4)
EcoT14I       218  CCAAGG               C^C[AT][AT]GG
Ksp632I       285  GAAGAG               CTCTTC(1/4)
MflI          143  AGATCT               [GA]^GATC[CT]
MroI          272  TCCGGA               T^CCGGA
MwoI          441  GCGAATCCAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PmaCI         724  CACGTG               CAC^GTG
PmlI          724  CACGTG               CAC^GTG
SapI          285  GAAGAGC              GCTCTTC(1/4)
SfcI          177  CTACAG               C^T[GA][CT]AG
SmlI          410  CTCAAG               C^T[CT][GA]AG
StyI          218  CCAAGG               C^C[AT][AT]GG
XhoII         143  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AciI           61  GCGG                 CCGC(-3/-1)
              450  GCGG                
AflIII        153  ACATGT               A^C[GA][CT]GT
              638  ACATGT              
AvaII           6  GGACC                G^G[AT]CC
              720  GGACC               
BcnI          519  CCCGG                CC^[GC]GG
              779  CCCGG               
BfaI          229  CTAG                 C^TAG
              808  CTAG                
BsaBI          69  GATTGCTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              792  GATAATTATC          
BspLU11I      153  ACATGT               A^CATGT
              638  ACATGT              
BssKI         519  CCCGG                ^CC[ACGT]GG
              779  CCCGG               
BstF5I         45  GGATG                GGATG(2/0)
              222  GGATG               
DpnII         144  GATC                 ^GATC
              540  GATC                
FokI           45  GGATG                GGATG(9/13)
              222  GGATG               
HphI          387  GGTGA                GGTGA(8/7)
              676  GGTGA               
MaeI          229  CTAG                 C^TAG
              808  CTAG                
MaeII         269  ACGT                 A^CGT
              725  ACGT                
MamI           69  GATTGCTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              792  GATAATTATC          
MboI          144  GATC                 ^GATC
              540  GATC                
MseI          131  TTAA                 T^TAA
              251  TTAA                
NciI          519  CCCGG                CC^[GC]GG
              779  CCCGG               
NdeII         144  GATC                 ^GATC
              540  GATC                
NspI          153  ACATGT               [GA]CATG^[CT]
              638  ACATGT              
PleI          547  GACTC                GAGTC(4/5)
              771  GAGTC               
PvuII          19  CAGCTG               CAG^CTG
               34  CAGCTG              
Sau3AI        144  GATC                 ^GATC
              540  GATC                
ScrFI         519  CCCGG                CC^[ACGT]GG
              779  CCCGG               
SfaNI          58  GATGC                GCATC(5/9)
              620  GCATC               
TaqI           77  TCGA                 T^CGA
              458  TCGA                
Tru9I         131  TTAA                 T^TAA
              251  TTAA                
TspRI         573  CAGTG                CA[GC]TG[ACGT][ACGT]^
              716  CAGTG               
TthHB8I        77  TCGA                 T^CGA
              458  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   22 enzymes) #
#########################################

AluI        BbvI        BpmI        BseRI       Cfr13I      
DdeI        Fnu4HI      HaeIII      HapII       HinfI       
HpaII       ItaI        MboII       MnlI        MslI        
MspA1I      MspI        NlaIII      Sau96I      TfiI        
TseI        Tsp509I     
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