Restriction map for
pF1KB6374
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# NO CUT ENZYMES ( 147 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
AlwNI AocI Aor51HI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BamHI BanI BbeI BbrPI
BbsI BbvCI BcgI BclI BfrI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaHI BseAI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspMI BsrBI BsrDI BsrFI BsrGI
BsrI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraIII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FauI FbaI FseI FspI HgaI
Hin1I HincII HindII HindIII HpaI
KasI KpnI KspI MfeI MluI
MroI MslI MunI MvnI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TfiI Tsp45I Tth111I Van91I XbaI
XcmI XhoI
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# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AcsI 132 GAATTT [GA]^AATT[CT]
AfaI 24 GTAC GT^AC
AflIII 20 ACATGT A^C[GA][CT]GT
Alw44I 682 GTGCAC G^TGCAC
ApaLI 682 GTGCAC G^TGCAC
ApoI 132 GAATTT [GA]^AATT[CT]
Asp700I 321 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 682 GTGCAC G[AT]GC[AT]^C
BciVI 755 GTATCC GTATCC(6/5)
BglI 39 GCCCTCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 571 CTGGAG CTGGAG(16/14)
BsaI 456 GGTCTC GGTCTC(1/5)
BsaWI 26 ACCGGA [AT]^CCGG[AT]
BsiHKAI 682 GTGCAC G[AT]GC[AT]^C
BspHI 317 TCATGA T^CATGA
BspLU11I 20 ACATGT A^CATGT
BstYI 412 GGATCT [GA]^GATC[CT]
CfoI 288 GCGC GCG^C
DraI 604 TTTAAA TTT^AAA
EarI 430 GAAGAG CTCTTC(1/4)
EcoT14I 221 CCAAGG C^C[AT][AT]GG
HaeII 287 AGCGCC [GA]GCGC^[CT]
HhaI 288 GCGC GCG^C
HinP1I 288 GCGC G^CGC
HinfI 163 GACTC G^A[ACGT]TC
HphI 637 GGTGA GGTGA(8/7)
Ksp632I 430 GAAGAG CTCTTC(1/4)
MflI 412 GGATCT [GA]^GATC[CT]
MwoI 39 GCCCTCGGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NspI 20 ACATGT [GA]CATG^[CT]
PleI 163 GACTC GAGTC(4/5)
PpuMI 253 GGGTCCT [GA]G^G[AT]CC[CT]
RcaI 317 TCATGA T^CATGA
RsaI 24 GTAC GT^AC
SfiI 38 GGCCCTCGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 33 CCCGGG CCC^GGG
SmlI 165 CTCAAG C^T[CT][GA]AG
StyI 221 CCAAGG C^C[AT][AT]GG
TaqI 689 TCGA T^CGA
TthHB8I 689 TCGA T^CGA
XhoII 412 GGATCT [GA]^GATC[CT]
XmaCI 33 CCCGGG C^CCGGG
XmaI 33 CCCGGG C^CCGGG
XmnI 321 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AciI 89 GCGG CCGC(-3/-1)
102 GCGG
ApaI 37 GGGCCC GGGCC^C
46 GGGCCC
AvaI 33 CCCGGG C^[CT]CG[GA]G
42 CTCGGG
AvaII 10 GGTCC G^G[AT]CC
254 GGTCC
BalI 512 TGGCCA TGG^CCA
614 TGGCCA
BanII 37 GGGCCC G[GA]GC[CT]^C
46 GGGCCC
BfaI 148 CTAG C^TAG
811 CTAG
BsaBI 589 GATGTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
750 GATGTGTATC
BseRI 445 GAGGAG GAGGAG(10/8)
475 GAGGAG
BsgI 84 GTGCAG GTGCAG(16/14)
358 GTGCAG
BsmAI 297 GAGAC GTCTC(1/5)
457 GTCTC
BsoBI 33 CCCGGG C^[CT]CG[GA]G
42 CTCGGG
EaeI 512 TGGCCA [CT]^GGCC[GA]
614 TGGCCA
MaeI 148 CTAG C^TAG
811 CTAG
MaeII 82 ACGT A^CGT
686 ACGT
MaeIII 195 GTTAC ^GT[ACGT]AC
708 GTTAC
MamI 589 GATGTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
750 GATGTGTATC
MboII 430 GAAGA GAAGA(8/7)
747 GAAGA
MluNI 512 TGGCCA TGG^CCA
614 TGGCCA
MscI 512 TGGCCA TGG^CCA
614 TGGCCA
PstI 332 CTGCAG CTGCA^G
425 CTGCAG
PvuII 451 CAGCTG CAG^CTG
568 CAGCTG
SfaNI 489 GATGC GCATC(5/9)
71 GCATC
#########################################
# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AluI AlwI BbvI BcnI BmyI
BsaJI BsiYI BslI BsmFI Bsp1286I
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII DraII EcoO109I EcoRII
Fnu4HI FokI HaeIII HapII HpaII
ItaI MboI MnlI MseI MspA1I
MspI MvaI NciI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI SfcI
Tru9I TseI Tsp509I TspRI