Restriction map for pF1KB7227
##################################
# NO CUT ENZYMES (  194 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwI        AlwNI       AocI        ApaI        
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspHI       AspI        AvaII       
AviII       AvrII       BalI        BamHI       BanI        
BbeI        BbrPI       BbsI        BbvCI       BbvI        
BcgI        BciVI       BclI        BcnI        BfrI        
BglI        BglII       BlnI        BlpI        Bpu1102I    
BpuAI       BsaAI       BsaBI       BsaHI       BsaI        
BsaWI       BseAI       BseRI       BsgI        BsiEI       
BsiHKAI     BsiWI       BsiYI       BslI        BsmBI       
BsmI        BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrBI       BsrFI       BssHII      BssKI       
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstNI       BstPI       BstYI       BstZ17I     Bsu36I      
Cac8I       CelII       Cfr10I      Cfr13I      ClaI        
CpoI        DpnII       DraI        DraII       DraIII      
DrdI        EaeI        EagI        Eam1105I    EarI        
EclXI       Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRI       EcoRII      EcoRV       EcoT22I     
FauI        FbaI        Fnu4HI      FseI        FspI        
HaeIII      HapII       HgaI        Hin1I       HincII      
HindII      HindIII     HpaI        HpaII       ItaI        
KasI        KpnI        Ksp632I     MaeIII      MamI        
MboI        MflI        MluI        MluNI       MroI        
MscI        MspI        MvaI        MwoI        NaeI        
NarI        NciI        NdeII       NgoMIV      NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PflFI       PflMI       PinAI       PleI        
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RcaI        RsrII       SacI        SalI        SapI        
Sau3AI      Sau96I      SbfI        ScaI        ScrFI       
SexAI       SfaNI       SfcI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SphI        
Sse8387I    SspI        StuI        SwaI        TseI        
Tsp45I      TspRI       Tth111I     Van91I      XcmI        
XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AccII         433  CGCG                 CG^CG
AcsI          124  AAATTC               [GA]^AATT[CT]
AluI          174  AGCT                 AG^CT
Aor51HI       353  AGCGCT               AGC^GCT
ApoI          124  AAATTC               [GA]^AATT[CT]
AvaI          323  CTCGAG               C^[CT]CG[GA]G
BanII         309  GGGCTC               G[GA]GC[CT]^C
BmyI          309  GGGCTC               G[AGT]GC[ACT]^C
BpmI          203  CTGGAG               CTGGAG(16/14)
BsmFI         537  GTCCC                GGGAC(10/14)
BsoBI         323  CTCGAG               C^[CT]CG[GA]G
Bsp1286I      309  GGGCTC               G[AGT]GC[ACT]^C
Bsp1407I      344  TGTACA               T^GTACA
BsrDI         230  CATTGC               GCAATG(2/0)
BsrGI         344  TGTACA               T^GTACA
BstUI         433  CGCG                 CG^CG
BstXI         444  CCATCCTTGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI          354  GCGC                 GCG^C
Eco47III      353  AGCGCT               AGC^GCT
EcoT14I       129  CCATGG               C^C[AT][AT]GG
HaeII         353  AGCGCT               [GA]GCGC^[CT]
HhaI          354  GCGC                 GCG^C
HinP1I        354  GCGC                 G^CGC
KspI          432  CCGCGG               CCGC^GG
MaeII         633  ACGT                 A^CGT
MfeI           14  CAATTG               C^AATTG
MseI           80  TTAA                 T^TAA
MspA1I        432  CCGCGG               C[AC]G^C[GT]G
MunI           14  CAATTG               C^AATTG
MvnI          433  CGCG                 CG^CG
NcoI          129  CCATGG               C^CATGG
NdeI          276  CATATG               CA^TATG
NlaIV         310  GGCTCC               GG[ACGT]^[ACGT]CC
PaeR7I        323  CTCGAG               C^TCGAG
SacII         432  CCGCGG               CCGC^GG
SmlI          323  CTCGAG               C^T[CT][GA]AG
SpeI          498  ACTAGT               A^CTAGT
SspBI         344  TGTACA               T^GTACA
StyI          129  CCATGG               C^C[AT][AT]GG
Tru9I          80  TTAA                 T^TAA
XbaI          118  TCTAGA               T^CTAGA
XhoI          323  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

AciI          432  CCGC                 CCGC(-3/-1)
              434  GCGG                
AfaI          250  GTAC                 GT^AC
              345  GTAC                
BfaI          119  CTAG                 C^TAG
              499  CTAG                
BsmAI         206  GAGAC                GTCTC(1/5)
              326  GAGAC               
BsrI          202  ACTGG                ACTGG(1/-1)
              107  CCAGT               
BstF5I        445  CATCC                GGATG(2/0)
              585  CATCC               
DdeI          339  CTTAG                C^T[ACGT]AG
              573  CTTAG               
FokI          445  CATCC                GGATG(9/13)
              585  CATCC               
HinfI         166  GAATC                G^A[ACGT]TC
              262  GATTC               
HphI          169  TCACC                GGTGA(8/7)
              466  TCACC               
MaeI          119  CTAG                 C^TAG
              499  CTAG                
MboII         475  GAAGA                GAAGA(8/7)
              418  TCTTC               
MslI          272  CATTCATATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              445  CATCCTTGTG          
RsaI          250  GTAC                 GT^AC
              345  GTAC                
TfiI          166  GAATC                G^A[AT]TC
              262  GATTC               


#########################################
# 3 OR MORE CUT ENZYMES (    7 enzymes) #
#########################################

BsaJI       DsaI        MnlI        NlaIII      TaqI        
Tsp509I     TthHB8I     
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