Restriction map for pF1KB6326
##################################
# NO CUT ENZYMES (  173 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwNI       Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp718I     
AspEI       AspHI       AspI        AvaI        AvaII       
AviII       AvrII       BalI        BamHI       BanI        
BbeI        BbrPI       BbvCI       BcgI        BciVI       
BclI        BcnI        BfrI        BlnI        BlpI        
BpmI        Bpu1102I    BsaAI       BsaBI       BsaHI       
BsaWI       BseAI       BsiEI       BsiHKAI     BsiWI       
BsmBI       BsmFI       BsmI        BsoBI       Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrFI       BsrGI       BssHII      Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstUI       
BstZ17I     CelII       CfoI        Cfr10I      ClaI        
CpoI        DraI        DraII       DrdI        EaeI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
EcoNI       EcoO109I    EcoO65I     EcoRI       EcoRV       
EcoT22I     FbaI        FseI        FspI        HaeII       
HapII       HgaI        HhaI        Hin1I       HinP1I      
HpaI        HpaII       KasI        KpnI        KspI        
MaeII       MamI        MfeI        MluI        MluNI       
MroI        MscI        MseI        MspA1I      MspI        
MunI        MvnI        NaeI        NarI        NciI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PvuI        PvuII       RcaI        RsrII       SacI        
SacII       SalI        SbfI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        TaqI        Tru9I       
Tsp45I      Tth111I     TthHB8I     Van91I      XcmI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

AcsI          405  GAATTT               [GA]^AATT[CT]
AlwI          847  GGATC                GGATC(4/5)
ApoI          405  GAATTT               [GA]^AATT[CT]
Asp700I       277  GAAGATTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanII         484  GAGCCC               G[GA]GC[CT]^C
BbsI          565  GTCTTC               GAAGAC(2/6)
BglI           71  GCCCCAGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII          99  AGATCT               A^GATCT
BpuAI         565  GTCTTC               GAAGAC(2/6)
BsaI          705  GAGACC               GGTCTC(1/5)
BseRI           7  CTCCTC               GAGGAG(10/8)
BsgI          245  GTGCAG               GTGCAG(16/14)
BsrDI         545  GCAATG               GCAATG(2/0)
BsrI          390  CCAGT                ACTGG(1/-1)
BssSI          37  CTCGTG               CACGAG(-5/-1)
BstXI         182  CCACAGATTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I          17  GCTTGC               GC[ACGT]^[ACGT]GC
Cfr13I         70  GGCCC                G^G[ACGT]CC
DraIII        239  CACCGAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI          488  CCATGG               C^C[GA][CT]GG
EcoT14I       488  CCATGG               C^C[AT][AT]GG
FauI           14  CCCGC                CCCGC(4/6)
HincII        751  GTTGAC               GT[CT]^[GA]AC
HindII        751  GTTGAC               GT[CT]^[GA]AC
MaeIII        580  GTTAC                ^GT[ACGT]AC
MslI          429  CACTGTAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MwoI           71  GCCCCAGAGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI          488  CCATGG               C^CATGG
NlaIV          70  GGCCCC               GG[ACGT]^[ACGT]CC
PleI            5  GACTC                GAGTC(4/5)
PstI          479  CTGCAG               CTGCA^G
SapI          415  GCTCTTC              GCTCTTC(1/4)
Sau96I         70  GGCCC                G^G[ACGT]CC
ScaI          464  AGTACT               AGT^ACT
StyI          488  CCATGG               C^C[AT][AT]GG
XbaI          691  TCTAGA               T^CTAGA
XmnI          277  GAAGATTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AocI          379  CCTCAGG              CC^T[ACGT]AGG
              769  CCTCAGG             
BbvI           21  GCTGC                GCAGC(8/12)
               24  GCTGC               
BfaI          685  CTAG                 C^TAG
              692  CTAG                
BmyI          484  GAGCCC               G[AGT]GC[ACT]^C
              827  GGGCAC              
Bsp1286I      484  GAGCCC               G[AGT]GC[ACT]^C
              827  GGGCAC              
BssKI         309  CCTGG                ^CC[ACGT]GG
              818  CCAGG               
BstNI         309  CCTGG                CC^[AT]GG
              818  CCAGG               
BstYI          99  AGATCT               [GA]^GATC[CT]
              847  GGATCT              
Bsu36I        379  CCTCAGG              CC^T[ACGT]AGG
              769  CCTCAGG             
EarI          416  CTCTTC               CTCTTC(1/4)
              834  GAAGAG              
Eco81I        379  CCTCAGG              CC^T[ACGT]AGG
              769  CCTCAGG             
EcoRII        309  CCTGG                ^CC[AT]GG
              818  CCAGG               
HaeIII         70  GGCC                 GG^CC
              670  GGCC                
HindIII       233  AAGCTT               A^AGCTT
              797  AAGCTT              
Ksp632I       416  CTCTTC               CTCTTC(1/4)
              834  GAAGAG              
MaeI          685  CTAG                 C^TAG
              692  CTAG                
MflI           99  AGATCT               [GA]^GATC[CT]
              847  GGATCT              
MvaI          309  CCTGG                CC^[AT]GG
              818  CCAGG               
NlaIII        489  CATG                 CATG^
              756  CATG                
ScrFI         309  CCTGG                CC^[ACGT]GG
              818  CCAGG               
SfaNI         361  GATGC                GCATC(5/9)
              559  GATGC               
SfcI          431  CTGTAG               C^T[GA][CT]AG
              479  CTGCAG              
TseI           21  GCTGC                G^C[AT]GC
               24  GCTGC               
Tsp509I       138  AATT                 ^AATT
              406  AATT                
XhoII          99  AGATCT               [GA]^GATC[CT]
              847  GGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   23 enzymes) #
#########################################

AciI        AfaI        AluI        BsaJI       BsiYI       
BslI        BsmAI       BstF5I      DdeI        DpnII       
Fnu4HI      FokI        HinfI       HphI        ItaI        
MboI        MboII       MnlI        NdeII       RsaI        
Sau3AI      TfiI        TspRI       
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