Restriction map for pF1KE0301
##################################
# NO CUT ENZYMES (  135 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AflII       AgeI        AhdI        
Alw44I      Aor51HI     ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp718I     AspEI       
AviII       AvrII       BamHI       BanI        BanII       
BbeI        BbrPI       BbvCI       BcgI        BclI        
BcnI        BfrI        BglI        BglII       BlnI        
BlpI        Bpu1102I    BsaBI       BsaHI       BsaI        
BseAI       BsiEI       BsiWI       BspDI       BspEI       
BsrBI       BsrFI       BssHII      BssSI       Bst1107I    
BstBI       BstEII      BstPI       BstXI       BstZ17I     
CelII       CfoI        Cfr10I      ClaI        CpoI        
DraI        DraIII      EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoNI       EcoO65I     EcoRI       
EcoRV       EcoT14I     EcoT22I     FbaI        FseI        
FspI        HaeII       HhaI        Hin1I       HinP1I      
HindIII     HphI        KasI        KpnI        KspI        
MamI        MfeI        MluI        MroI        MunI        
NaeI        NarI        NciI        NcoI        NgoMIV      
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflMI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    StuI        StyI        SwaI        Van91I      
XbaI        XcmI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AccII         117  CGCG                 CG^CG
AocI         1076  CCTTAGG              CC^T[ACGT]AGG
AspHI         374  GAGCAC               G[AT]GC[AT]^C
AspI          154  GACGTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI          203  TGGCCA               TGG^CCA
BbsI         1091  GAAGAC               GAAGAC(2/6)
BpuAI        1091  GAAGAC               GAAGAC(2/6)
BsaAI         796  TACGTG               [CT]AC^GT[GA]
BsaWI        1041  ACCGGA               [AT]^CCGG[AT]
BseRI        1271  CTCCTC               GAGGAG(10/8)
BsgI           61  GTGCAG               GTGCAG(16/14)
BsiHKAI       374  GAGCAC               G[AT]GC[AT]^C
BsmBI         103  GAGACG               CGTCTC(1/5)
BsmFI         160  GTCCC                GGGAC(10/14)
BsmI          588  GCATTC               GAATGC(1/-1)
Bsp1407I     1157  TGTACA               T^GTACA
BspHI         941  TCATGA               T^CATGA
BspMI          12  GCAGGT               ACCTGC(4/8)
BsrDI         982  GCAATG               GCAATG(2/0)
BsrGI        1157  TGTACA               T^GTACA
BstAPI         12  GCAGGTGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI         117  CGCG                 CG^CG
Bsu36I       1076  CCTTAGG              CC^T[ACGT]AGG
DrdI          412  GACCCTGGAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI          719  CCGTGG               C^C[GA][CT]GG
EaeI          203  TGGCCA               [CT]^GGCC[GA]
EarI          143  CTCTTC               CTCTTC(1/4)
Eco81I       1076  CCTTAGG              CC^T[ACGT]AGG
FauI          118  GCGGG                CCCGC(4/6)
HapII        1042  CCGG                 C^CGG
HgaI          105  GACGC                GACGC(5/10)
HpaI          868  GTTAAC               GTT^AAC
HpaII        1042  CCGG                 C^CGG
Ksp632I       143  CTCTTC               CTCTTC(1/4)
MluNI         203  TGGCCA               TGG^CCA
MscI          203  TGGCCA               TGG^CCA
MslI          939  CATCATGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI         1042  CCGG                 C^CGG
MvnI          117  CGCG                 CG^CG
NdeI          393  CATATG               CA^TATG
NheI          348  GCTAGC               G^CTAGC
PflFI         154  GACGTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
RcaI          941  TCATGA               T^CATGA
ScaI          516  AGTACT               AGT^ACT
SexAI        1395  ACCTGGT              A^CC[AT]GGT
SspBI        1157  TGTACA               T^GTACA
SspI         1279  AATATT               AAT^ATT
TfiI          285  GAATC                G^A[AT]TC
Tth111I       154  GACGTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AflIII       1160  ACATGT               A^C[GA][CT]GT
             1290  ACATGT              
Asp700I       285  GAATCGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1252  GAACCATTTC          
AvaI          847  CCCGAG               C^[CT]CG[GA]G
             1444  CCCGAG              
BciVI         381  GGATAC               GTATCC(6/5)
              524  GTATCC              
BmyI          374  GAGCAC               G[AGT]GC[ACT]^C
              440  GGGCAC              
BpmI          593  CTCCAG               CTGGAG(16/14)
              416  CTGGAG              
BsiYI         911  CCTGCAAAGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              978  CCTAGCAATGG         
BslI          911  CCTGCAAAGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              978  CCTAGCAATGG         
BsmAI         103  GAGAC                GTCTC(1/5)
              198  GAGAC               
BsoBI         847  CCCGAG               C^[CT]CG[GA]G
             1444  CCCGAG              
Bsp1286I      374  GAGCAC               G[AGT]GC[ACT]^C
              440  GGGCAC              
BspLU11I     1160  ACATGT               A^CATGT
             1290  ACATGT              
Cac8I          78  GCTGGC               GC[ACGT]^[ACGT]GC
              348  GCTAGC              
DraII          49  AGGACCC              [GA]G^G[ACGT]CC[CT]
              907  GGGTCCT             
EcoO109I       49  AGGACCC              [GA]G^G[ACGT]CC[CT]
              907  GGGTCCT             
HincII        868  GTTAAC               GT[CT]^[GA]AC
             1164  GTCAAC              
HindII        868  GTTAAC               GT[CT]^[GA]AC
             1164  GTCAAC              
MaeII         155  ACGT                 A^CGT
              797  ACGT                
NspI         1160  ACATGT               [GA]CATG^[CT]
             1290  ACATGT              
PleI            3  GAGTC                GAGTC(4/5)
              419  GAGTC               
PpuMI          49  AGGACCC              [GA]G^G[AT]CC[CT]
              907  GGGTCCT             
Tsp45I        477  GTCAC                ^GT[GC]AC
             1126  GTCAC               
TspRI         377  CACTG                CA[GC]TG[ACGT][ACGT]^
              573  CAGTG               
XmnI          285  GAATCGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1252  GAACCATTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   50 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AlwNI       
AvaII       BbvI        BfaI        BsaJI       BsrI        
BssKI       BstF5I      BstNI       BstYI       Cfr13I      
DdeI        DpnII       EcoRII      Fnu4HI      FokI        
HaeIII      HinfI       ItaI        MaeI        MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MspA1I      MvaI        MwoI        NdeII       NlaIII      
NlaIV       PstI        PvuII       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        TaqI        
Tru9I       TseI        Tsp509I     TthHB8I     XhoII       
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