Restriction map for pF1KE0301
 (Linear) (Six-base) MAPSORT of: KIEE0301.seq  Check: 4704  from: 1  to: 1527



KIEE0301.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:12  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0       3    1527 
   Size:          3    1524
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     799    1527 
   Size:        799     728
 
AflIII A'CryG_T

Cuts at:      0    1160    1290    1527 
   Size:       1160     130     237

  Fragments arranged by size: 

                1160     237     130
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     961    1527 
   Size:        961     566
 
AlwNI CAG_nnn'CTG

Cuts at:      0      18     232     502     628    1396    1527 
   Size:         18     214     270     126     768     131

  Fragments arranged by size: 

                 768     270     214     131     126      18
 
AvaI C'yCGr_G

Cuts at:      0     847    1444    1527 
   Size:        847     597      83

  Fragments arranged by size: 

                 847     597      83
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     374     407    1527 
   Size:        374      33    1120

  Fragments arranged by size: 

                1120     374      33
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1098    1527 
   Size:       1098     429
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     375     535    1527 
   Size:        375     160     992

  Fragments arranged by size: 

                 992     375     160
 
Bme1580I G_kGCm'C

Cuts at:      0     444    1527 
   Size:        444    1083
 
BmgBI CAC'GTC

Cuts at:      0     156    1527 
   Size:        156    1371
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      48    1008    1527 
   Size:         48     960     519

  Fragments arranged by size: 

                 960     519      48
 
BmtI G_CTAG'C

Cuts at:      0     352    1527 
   Size:        352    1175
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     437     578    1527 
   Size:        437     141     949

  Fragments arranged by size: 

                 949     437     141
 
Bpu10I CC'TnA_GC

Cuts at:      0     353    1527 
   Size:        353    1174
 
BsaAI yAC'GTr

Cuts at:      0     798    1527 
   Size:        798     729
 
BsaWI w'CCGG_w

Cuts at:      0    1041    1527 
   Size:       1041     486
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1262    1527 
   Size:       1262     265
 
BseYI C'CCAG_C

Cuts at:      0     344    1527 
   Size:        344    1183
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      82    1527 
   Size:         82    1445
 
BsiHKAI G_wGCw'C

Cuts at:      0     378    1527 
   Size:        378    1149
 
BsmI GAATG_Cn'

Cuts at:      0     588    1527 
   Size:        588     939
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      97    1527 
   Size:         97    1430
 
Bsp1286I G_dGCh'C

Cuts at:      0     378     444    1527 
   Size:        378      66    1083

  Fragments arranged by size: 

                1083     378      66
 
BspHI T'CATG_A

Cuts at:      0     941    1527 
   Size:        941     586
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0       3    1527 
   Size:          3    1524
 
BsrDI GCAATG_nn'

Cuts at:      0     989    1527 
   Size:        989     538
 
BsrGI T'GTAC_A

Cuts at:      0    1157    1527 
   Size:       1157     370
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      18    1527 
   Size:         18    1509
 
BstYI r'GATC_y

Cuts at:      0     683     723    1182    1527 
   Size:        683      40     459     345

  Fragments arranged by size: 

                 683     459     345      40
 
Bsu36I CC'TnA_GG

Cuts at:      0    1077    1527 
   Size:       1077     450
 
BtgI C'CryG_G

Cuts at:      0     719    1527 
   Size:        719     808
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     418    1527 
   Size:        418    1109
 
EaeI y'GGCC_r

Cuts at:      0     203    1527 
   Size:        203    1324
 
EarI CTCTTCn'nnn_

Cuts at:      0     149    1527 
   Size:        149    1378
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      88    1527 
   Size:         88    1439
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     437     578     799    1527 
   Size:        437     141     221     728

  Fragments arranged by size: 

                 728     437     221     141
 
EcoO109I rG'GnC_Cy

Cuts at:      0      50     908    1527 
   Size:         50     858     619

  Fragments arranged by size: 

                 858     619      50
 
HincII GTy'rAC

Cuts at:      0     870    1166    1527 
   Size:        870     296     361

  Fragments arranged by size: 

                 870     361     296
 
HpaI GTT'AAC

Cuts at:      0     870    1527 
   Size:        870     657
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1278    1527 
   Size:       1278     249
 
MscI TGG'CCA

Cuts at:      0     205    1527 
   Size:        205    1322
 
MslI CAynn'nnrTG

Cuts at:      0     943    1527 
   Size:        943     584
 
MspA1I CmG'CkG

Cuts at:      0     307     861    1066    1527 
   Size:        307     554     205     461

  Fragments arranged by size: 

                 554     461     307     205
 
NdeI CA'TA_TG

Cuts at:      0     394    1527 
   Size:        394    1133
 
NheI G'CTAG_C

Cuts at:      0     348    1527 
   Size:        348    1179
 
NspI r_CATG'y

Cuts at:      0    1164    1294    1527 
   Size:       1164     130     233

  Fragments arranged by size: 

                1164     233     130
 
PciI A'CATG_T

Cuts at:      0    1160    1290    1527 
   Size:       1160     130     237

  Fragments arranged by size: 

                1160     237     130
 
PfoI T'CCnGG_A

Cuts at:      0     527    1527 
   Size:        527    1000
 
PpuMI rG'GwC_Cy

Cuts at:      0      50     908    1527 
   Size:         50     858     619

  Fragments arranged by size: 

                 858     619      50
 
PsiI TTA'TAA

Cuts at:      0     212    1527 
   Size:        212    1315
 
PstI C_TGCA'G

Cuts at:      0     113     435    1065    1496    1527 
   Size:        113     322     630     431      31

  Fragments arranged by size: 

                 630     431     322     113      31
 
PvuII CAG'CTG

Cuts at:      0     307     861    1066    1527 
   Size:        307     554     205     461

  Fragments arranged by size: 

                 554     461     307     205
 
ScaI AGT'ACT

Cuts at:      0     518    1527 
   Size:        518    1009
 
SexAI A'CCwGG_T

Cuts at:      0    1395    1527 
   Size:       1395     132
 
SfcI C'TryA_G

Cuts at:      0     109     431    1061    1492    1527 
   Size:        109     322     630     431      35

  Fragments arranged by size: 

                 630     431     322     109      35
 
SspI AAT'ATT

Cuts at:      0    1281    1527 
   Size:       1281     246
 
TatI w'GTAC_w

Cuts at:      0     516     765    1157    1527 
   Size:        516     249     392     370

  Fragments arranged by size: 

                 516     392     370     249
 
Tth111I GACn'n_nGTC

Cuts at:      0     157    1527 
   Size:        157    1370
 
XmnI GAAnn'nnTTC

Cuts at:      0     289    1256    1527 
   Size:        289     967     271

  Fragments arranged by size: 

                 967     289     271
 

 Enzymes that do cut:

     AarI     AcuI   AflIII     AlfI    AlwNI     AvaI     BaeI     BbsI
    BciVI Bme1580I    BmgBI     BmrI     BmtI     BpmI   Bpu10I    BsaAI
    BsaWI    BseRI    BseYI     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I
    BspHI    BspMI    BsrDI    BsrGI   BstAPI    BstYI   Bsu36I     BtgI
     DrdI     EaeI     EarI     EciI  Eco57MI EcoO109I   HincII     HpaI
     MmeI     MscI     MslI   MspA1I     NdeI     NheI     NspI     PciI
     PfoI    PpuMI     PsiI     PstI    PvuII     ScaI    SexAI     SfcI
     SspI     TatI  Tth111I     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AloI     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI
    AvrII    BamHI     BanI    BanII     BbeI    BbvCI     BcgI     BclI
    BfrBI     BglI    BglII     BlpI     BplI    BpuEI     BsaI    BsaBI
    BsaHI    BsaXI    BsiEI    BsiWI    BspEI    BsrBI    BsrFI   BssHII
    BssSI    BstBI   BstEII    BstXI  BstZ17I    BtgZI     BtsI     ClaI
    CspCI     DraI   DraIII     EagI  EcoICRI    EcoNI    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII    Hin4I  HindIII     KasI
     KpnI     MfeI     MluI     NaeI     NarI     NcoI   NgoMIV     NotI
     NruI     NsiI     PacI     PasI    PflMI     PmeI     PmlI     PpiI
    PshAI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     StuI     StyI
     SwaI    TaqII     TstI     XbaI     XcmI     XhoI     XmaI     ZraI
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