Restriction map for pF1KB6712
##################################
# NO CUT ENZYMES (  197 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AfaI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwNI       
AocI        Aor51HI     ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AvaI        AvaII       
AviII       AvrII       BanII       BbeI        BbrPI       
BbsI        BbvCI       BcgI        BciVI       BclI        
BcnI        BfaI        BfrI        BglI        BglII       
BlnI        BlpI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaHI       BsaI        BsaWI       BseAI       
BseRI       BsgI        BsiEI       BsiHKAI     BsiWI       
BsmAI       BsmBI       BsmFI       BsmI        BsoBI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrBI       BsrDI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstBI       BstEII      
BstPI       BstUI       BstXI       BstZ17I     Bsu36I      
CelII       CfoI        Cfr10I      Cfr13I      ClaI        
CpoI        DraI        DraII       DraIII      DrdI        
DsaI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO109I    EcoO65I     
EcoRI       EcoRV       EcoT22I     FauI        FbaI        
FseI        FspI        HaeII       HapII       HgaI        
HhaI        Hin1I       HinP1I      HindIII     HpaI        
HpaII       HphI        KasI        KpnI        KspI        
MaeI        MaeII       MamI        MfeI        MluI        
MroI        MspI        MunI        MvnI        NaeI        
NarI        NciI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RcaI        RsaI        RsrII       SacI        SacII       
SalI        SapI        Sau96I      SbfI        ScaI        
SexAI       SfcI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        TaqI        TfiI        Tth111I     TthHB8I     
Van91I      XbaI        XcmI        XhoI        XmaCI       
XmaI        XmnI        


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# ONE CUT ENZYMES (   32 enzymes) #
###################################

AciI           11  GCGG                 CCGC(-3/-1)
BamHI         219  GGATCC               G^GATCC
BanI           54  GGCACC               G^G[CT][GA]CC
BbvI           33  GCTGC                GCAGC(8/12)
BmyI          264  GTGCCC               G[AGT]GC[ACT]^C
BpmI           95  CTGGAG               CTGGAG(16/14)
BsiYI          21  CCCCTTCCTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI           21  CCCCTTCCTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1286I      264  GTGCCC               G[AGT]GC[ACT]^C
BstAPI        206  GCATCAAATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I        321  GGATG                GGATG(2/0)
BstYI         219  GGATCC               [GA]^GATC[CT]
DpnII         220  GATC                 ^GATC
EcoT14I        58  CCTTGG               C^C[AT][AT]GG
FokI          321  GGATG                GGATG(9/13)
HincII        226  GTTGAC               GT[CT]^[GA]AC
HindII        226  GTTGAC               GT[CT]^[GA]AC
HinfI          98  GAGTC                G^A[ACGT]TC
MboI          220  GATC                 ^GATC
MflI          219  GGATCC               [GA]^GATC[CT]
MseI          296  TTAA                 T^TAA
MslI          354  CATGGGCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I          9  CAGCGG               C[AC]G^C[GT]G
NdeII         220  GATC                 ^GATC
NspI          359  GCATGT               [GA]CATG^[CT]
PleI           98  GAGTC                GAGTC(4/5)
Sau3AI        220  GATC                 ^GATC
StyI           58  CCTTGG               C^C[AT][AT]GG
Tru9I         296  TTAA                 T^TAA
TseI           33  GCTGC                G^C[AT]GC
Tsp509I       307  AATT                 ^AATT
XhoII         219  GGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

AluI           93  AGCT                 AG^CT
              393  AGCT                
AlwI          220  GATCC                GGATC(4/5)
              219  GGATC               
BalI          279  TGGCCA               TGG^CCA
              324  TGGCCA              
Cac8I          36  GCTTGC               GC[ACGT]^[ACGT]GC
              332  GCAAGC              
EaeI          279  TGGCCA               [CT]^GGCC[GA]
              324  TGGCCA              
EarI           16  CTCTTC               CTCTTC(1/4)
              180  GAAGAG              
Fnu4HI         11  GCGGC                GC^[ACGT]GC
               33  GCTGC               
ItaI           11  GCGGC                GC^[ACGT]GC
               33  GCTGC               
Ksp632I        16  CTCTTC               CTCTTC(1/4)
              180  GAAGAG              
MluNI         279  TGGCCA               TGG^CCA
              324  TGGCCA              
MscI          279  TGGCCA               TGG^CCA
              324  TGGCCA              
MwoI           55  GCACCTTGGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              206  GCATCAAATGC         
NlaIII        354  CATG                 CATG^
              360  CATG                
NlaIV          54  GGCACC               GG[ACGT]^[ACGT]CC
              219  GGATCC              
SfaNI         291  GATGC                GCATC(5/9)
              206  GCATC               


#########################################
# 3 OR MORE CUT ENZYMES (   14 enzymes) #
#########################################

BsaJI       BsrI        BssKI       BstNI       DdeI        
EcoRII      HaeIII      MaeIII      MboII       MnlI        
MvaI        ScrFI       Tsp45I      TspRI       
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