Restriction map for pF1KE0038
##################################
# NO CUT ENZYMES (  142 enzymes) #
##################################

AatII       AccII       AccIII      AciI        AclI        
AcyI        AflII       AgeI        AhdI        Alw44I      
AlwNI       Aor51HI     ApaI        ApaLI       AscI        
AspEI       AspI        AvaI        AviII       AvrII       
BalI        BamHI       BbeI        BbrPI       BbvCI       
BcgI        BciVI       BclI        BcnI        BfrI        
BglI        BlnI        BlpI        Bpu1102I    BsaHI       
BsaWI       BseAI       BseRI       BsgI        BsiEI       
BsiWI       BsmBI       BsmFI       BsoBI       BspDI       
BspEI       BspHI       BspMI       BsrBI       BssHII      
BssKI       BssSI       Bst1107I    BstBI       BstEII      
BstNI       BstPI       BstUI       BstXI       BstZ17I     
CelII       CfoI        ClaI        CpoI        DraII       
DrdI        DsaI        EaeI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      EcoNI       EcoO109I    
EcoO65I     EcoRII      EcoT22I     FauI        FbaI        
FseI        FspI        HaeII       HhaI        Hin1I       
HinP1I      HpaI        KasI        KspI        MluI        
MluNI       MroI        MscI        MvaI        MvnI        
NarI        NciI        NcoI        NdeI        NheI        
NotI        NruI        NsiI        NspV        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       Psp1406I    PstI        PvuI        
RcaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        ScrFI       SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspI        
StuI        SwaI        Tth111I     XbaI        XhoI        
XmaCI       XmaI        


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# ONE CUT ENZYMES (   46 enzymes) #
###################################

Acc65I       1654  GGTACC               G^GTACC
AccI         1870  GTCTAC               GT^[AC][GT]AC
AocI          745  CCTAAGG              CC^T[ACGT]AGG
Asp718I      1654  GGTACC               G^GTACC
BanI         1654  GGTACC               G^G[CT][GA]CC
BanII        1775  GGGCTC               G[GA]GC[CT]^C
BbsI          520  GTCTTC               GAAGAC(2/6)
BglII         720  AGATCT               A^GATCT
BpmI         1534  CTCCAG               CTGGAG(16/14)
BpuAI         520  GTCTTC               GAAGAC(2/6)
BsaAI         700  TACGTG               [CT]AC^GT[GA]
BsaI         1531  GGTCTC               GGTCTC(1/5)
Bsp1407I     1588  TGTACA               T^GTACA
BsrFI        1306  GCCGGC               [GA]^CCGG[CT]
BsrGI        1588  TGTACA               T^GTACA
BstAPI       1111  GCATCTCATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I        745  CCTAAGG              CC^T[ACGT]AGG
Cac8I        1306  GCCGGC               GC[ACGT]^[ACGT]GC
Cfr10I       1306  GCCGGC               [GA]^CCGG[CT]
DraI         1078  TTTAAA               TTT^AAA
DraIII        125  CACTAGGTG            CAC[ACGT][ACGT][ACGT]^GTG
EarI          186  CTCTTC               CTCTTC(1/4)
Eco81I        745  CCTAAGG              CC^T[ACGT]AGG
EcoRV        1248  GATATC               GAT^ATC
HaeIII       1309  GGCC                 GG^CC
HapII        1307  CCGG                 C^CGG
HgaI           65  GACGC                GACGC(5/10)
HincII       1671  GTTGAC               GT[CT]^[GA]AC
HindII       1671  GTTGAC               GT[CT]^[GA]AC
HindIII       213  AAGCTT               A^AGCTT
HpaII        1307  CCGG                 C^CGG
KpnI         1654  GGTACC               GGTAC^C
Ksp632I       186  CTCTTC               CTCTTC(1/4)
MfeI         1389  CAATTG               C^AATTG
MspA1I       1815  CAGCTG               C[AC]G^C[GT]G
MspI         1307  CCGG                 C^CGG
MunI         1389  CAATTG               C^AATTG
NaeI         1306  GCCGGC               GCC^GGC
NgoMIV       1306  GCCGGC               G^CCGGC
PacI          427  TTAATTAA             TTAAT^TAA
PvuII        1815  CAGCTG               CAG^CTG
SmlI         1473  CTCAAG               C^T[CT][GA]AG
SspBI        1588  TGTACA               T^GTACA
TaqI         1243  TCGA                 T^CGA
TthHB8I      1243  TCGA                 T^CGA
XcmI          560  CCATAGTACCATTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AseI          385  ATTAAT               AT^TAAT
              430  ATTAAT              
AsnI          385  ATTAAT               AT^TAAT
              430  ATTAAT              
AspHI         726  GAGCAC               G[AT]GC[AT]^C
             1823  GAGCAC              
BsaBI         910  GATAGAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1463  GATGCAAATC          
BsaJI         939  CCAAGG               C^C[ACGT][ACGT]GG
             1967  CCAAGG              
BsiHKAI       726  GAGCAC               G[AT]GC[AT]^C
             1823  GAGCAC              
BsmAI         302  GTCTC                GTCTC(1/5)
             1532  GTCTC               
BsmI          636  GAATGC               GAATGC(1/-1)
             1567  GAATGC              
EcoRI        1751  GAATTC               G^AATTC
             2014  GAATTC              
EcoT14I       939  CCAAGG               C^C[AT][AT]GG
             1967  CCAAGG              
MaeII         701  ACGT                 A^CGT
             1073  ACGT                
MamI          910  GATAGAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1463  GATGCAAATC          
NlaIV        1410  GGAACC               GG[ACGT]^[ACGT]CC
             1654  GGTACC              
PleI          751  GACTC                GAGTC(4/5)
               34  GAGTC               
PshBI         385  ATTAAT               AT^TAAT
              430  ATTAAT              
SfcI         1509  CTGTAG               C^T[GA][CT]AG
             1886  CTATAG              
StyI          939  CCAAGG               C^C[AT][AT]GG
             1967  CCAAGG              


#########################################
# 3 OR MORE CUT ENZYMES (   53 enzymes) #
#########################################

AcsI        AfaI        AflIII      AluI        AlwI        
ApoI        Asp700I     AvaII       BbvI        BfaI        
BmyI        BsiYI       BslI        Bsp1286I    BspLU11I    
BsrDI       BsrI        BstF5I      BstYI       Cfr13I      
DdeI        DpnII       Fnu4HI      FokI        HinfI       
HphI        ItaI        MaeI        MaeIII      MboI        
MboII       MflI        MnlI        MseI        MslI        
MwoI        NdeII       NlaIII      NspI        PflMI       
RsaI        Sau3AI      Sau96I      SfaNI       TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
Van91I      XhoII       XmnI        
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