Restriction map for pF1KE0360
##################################
# NO CUT ENZYMES (  101 enzymes) #
##################################

AatII       AccII       AccIII      AclI        AflII       
AgeI        AocI        ApaI        AscI        AseI        
AsnI        Asp700I     AspI        AviII       AvrII       
BamHI       BbvCI       BciVI       BfrI        BglII       
BlnI        BlpI        Bpu1102I    BsaWI       BseAI       
BsiEI       BsiWI       BsmBI       BspEI       BspMI       
BsrBI       BsrFI       BssHII      Bst1107I    BstAPI      
BstBI       BstUI       BstXI       BstZ17I     Bsu36I      
CelII       Cfr10I      CpoI        DraI        EagI        
EclXI       Eco52I      Eco81I      EcoRI       EcoRV       
EcoT22I     FauI        FseI        FspI        HincII      
HindII      HpaI        KspI        MluI        MroI        
MvnI        NaeI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmeI        PshAI       
PshBI       Psp1406I    PvuI        RsrII       SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspI        SwaI        
Tth111I     XbaI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   63 enzymes) #
###################################

Acc65I       1038  GGTACC               G^GTACC
AccI          427  GTCTAC               GT^[AC][GT]AC
AcsI         1628  GAATTT               [GA]^AATT[CT]
AcyI          211  GGCGCC               G[GA]^CG[CT]C
AhdI          419  GACTCCAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI          341  GGATC                GGATC(4/5)
AlwNI        1542  CAGTAACTG            CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI       896  AGCGCT               AGC^GCT
ApoI         1628  GAATTT               [GA]^AATT[CT]
Asp718I      1038  GGTACC               G^GTACC
AspEI         419  GACTCCAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI          773  CTCGGG               C^[CT]CG[GA]G
BbeI          211  GGCGCC               GGCGC^C
BbsI          144  GAAGAC               GAAGAC(2/6)
BcgI         1713  CGATATAGATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI          998  TGATCA               T^GATCA
BcnI          991  CCGGG                CC^[GC]GG
BfaI         1379  CTAG                 C^TAG
BglI          902  GCCTGTGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI         144  GAAGAC               GAAGAC(2/6)
BsaBI        1657  GATGTACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI         211  GGCGCC               G[GA]^CG[CT]C
BsaI           34  GAGACC               GGTCTC(1/5)
BsoBI         773  CTCGGG               C^[CT]CG[GA]G
BspDI        1711  ATCGAT               AT^CGAT
BspHI        1169  TCATGA               T^CATGA
BspLU11I      917  ACATGT               A^CATGT
BsrDI        1480  GCAATG               GCAATG(2/0)
BssSI        1902  CTCGTG               CACGAG(-5/-1)
BstEII       1617  GGTCACC              G^GT[ACGT]ACC
BstPI        1617  GGTCACC              G^GT[ACGT]ACC
BstYI         341  GGATCT               [GA]^GATC[CT]
ClaI         1711  ATCGAT               AT^CGAT
DraIII        247  CACTATGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI           82  GACATCTCAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI          823  CCATGG               C^C[GA][CT]GG
Eam1105I      419  GACTCCAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III      896  AGCGCT               AGC^GCT
EcoNI        1901  CCTCGTGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I      1617  GGTCACC              G^GT[ACGT]ACC
FbaI          998  TGATCA               T^GATCA
HgaI         1249  GCGTC                GACGC(5/10)
Hin1I         211  GGCGCC               G[GA]^CG[CT]C
HindIII      1802  AAGCTT               A^AGCTT
KasI          211  GGCGCC               G^GCGCC
KpnI         1038  GGTACC               GGTAC^C
MaeI         1379  CTAG                 C^TAG
MamI         1657  GATGTACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         1397  CAATTG               C^AATTG
MflI          341  GGATCT               [GA]^GATC[CT]
MspA1I       1403  CAGCTG               C[AC]G^C[GT]G
MunI         1397  CAATTG               C^AATTG
NarI          211  GGCGCC               GG^CGCC
NciI          991  CCGGG                CC^[GC]GG
NcoI          823  CCATGG               C^CATGG
PflMI         246  CCACTATGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI          476  CTGCAG               CTGCA^G
PvuII        1403  CAGCTG               CAG^CTG
RcaI         1169  TCATGA               T^CATGA
SfoI          211  GGCGCC               GGC^GCC
StuI         1137  AGGCCT               AGG^CCT
Van91I        246  CCACTATGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII         341  GGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   31 enzymes) #
###################################

AflIII        917  ACATGT               A^C[GA][CT]GT
             1886  ACACGT              
Alw44I        873  GTGCAC               G^TGCAC
             1570  GTGCAC              
ApaLI         873  GTGCAC               G^TGCAC
             1570  GTGCAC              
BalI            2  TGGCCA               TGG^CCA
              826  TGGCCA              
BanI          211  GGCGCC               G^G[CT][GA]CC
             1038  GGTACC              
BanII        1173  GAGCTC               G[GA]GC[CT]^C
             1261  GAGCTC              
BbrPI         925  CACGTG               CAC^GTG
             1784  CACGTG              
BsgI         1080  CTGCAC               GTGCAG(16/14)
              892  GTGCAG              
BsmAI          34  GAGAC                GTCTC(1/5)
              921  GTCTC               
Bsp1407I     1013  TGTACA               T^GTACA
             1659  TGTACA              
BsrGI        1013  TGTACA               T^GTACA
             1659  TGTACA              
DpnII         342  GATC                 ^GATC
              999  GATC                
HapII         385  CCGG                 C^CGG
              991  CCGG                
HpaII         385  CCGG                 C^CGG
              991  CCGG                
MboI          342  GATC                 ^GATC
              999  GATC                
MluNI           2  TGGCCA               TGG^CCA
              826  TGGCCA              
MscI            2  TGGCCA               TGG^CCA
              826  TGGCCA              
MspI          385  CCGG                 C^CGG
              991  CCGG                
MwoI          556  GCTATGACTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              902  GCCTGTGTGGC         
NdeII         342  GATC                 ^GATC
              999  GATC                
NspI          917  ACATGT               [GA]CATG^[CT]
             1030  GCATGT              
PmaCI         925  CACGTG               CAC^GTG
             1784  CACGTG              
PmlI          925  CACGTG               CAC^GTG
             1784  CACGTG              
PpuMI        1319  GGGTCCC              [GA]G^G[AT]CC[CT]
             1833  GGGACCC             
SacI         1173  GAGCTC               GAGCT^C
             1261  GAGCTC              
Sau3AI        342  GATC                 ^GATC
              999  GATC                
SfcI          161  CTATAG               C^T[GA][CT]AG
              476  CTGCAG              
SspBI        1013  TGTACA               T^GTACA
             1659  TGTACA              
TaqI          731  TCGA                 T^CGA
             1712  TCGA                
Tsp45I        808  GTGAC                ^GT[GC]AC
             1618  GTCAC               
TthHB8I       731  TCGA                 T^CGA
             1712  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   63 enzymes) #
#########################################

AciI        AfaI        AluI        AspHI       AvaII       
BbvI        BmyI        BpmI        BsaAI       BsaJI       
BseRI       BsiHKAI     BsiYI       BslI        BsmFI       
BsmI        Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       Cac8I       CfoI        Cfr13I      DdeI        
DraII       EaeI        EarI        EcoO109I    EcoRII      
EcoT14I     Fnu4HI      FokI        HaeII       HaeIII      
HhaI        HinP1I      HinfI       HphI        ItaI        
Ksp632I     MaeII       MaeIII      MboII       MnlI        
MseI        MslI        MvaI        NlaIII      NlaIV       
PleI        RsaI        Sau96I      ScrFI       SfaNI       
SmlI        StyI        TfiI        Tru9I       TseI        
Tsp509I     TspRI       XcmI        
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com