Restriction map for pF1KE0360
 (Linear) (Six-base) MAPSORT of: KIEE0360.seq  Check: 3726  from: 1  to: 1977



KIEE0360.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:13  ..

AccI GT'mk_AC

Cuts at:      0     428    1977 
   Size:        428    1549
 
Acc65I G'GTAC_C

Cuts at:      0    1038    1977 
   Size:       1038     939
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     163     263     979    1977 
   Size:        163     100     716     998

  Fragments arranged by size: 

                 998     716     163     100
 
AfeI AGC'GCT

Cuts at:      0     898    1977 
   Size:        898    1079
 
AflIII A'CryG_T

Cuts at:      0     917    1886    1977 
   Size:        917     969      91

  Fragments arranged by size: 

                 969     917      91
 
AhdI GACnn_n'nnGTC

Cuts at:      0     424    1977 
   Size:        424    1553
 
AleI CACnn'nnGTG

Cuts at:      0    1105    1977 
   Size:       1105     872
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1547    1977 
   Size:       1547     430
 
ApaLI G'TGCA_C

Cuts at:      0     873    1570    1977 
   Size:        873     697     407

  Fragments arranged by size: 

                 873     697     407
 
ApoI r'AATT_y

Cuts at:      0    1628    1977 
   Size:       1628     349
 
AvaI C'yCGr_G

Cuts at:      0     773    1977 
   Size:        773    1204
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1651    1684    1977 
   Size:       1651      33     293

  Fragments arranged by size: 

                1651     293      33
 
BanI G'GyrC_C

Cuts at:      0     211    1038    1977 
   Size:        211     827     939

  Fragments arranged by size: 

                 939     827     211
 
BanII G_rGCy'C

Cuts at:      0    1177    1265    1977 
   Size:       1177      88     712

  Fragments arranged by size: 

                1177     712      88
 
BbeI G_GCGC'C

Cuts at:      0     215    1977 
   Size:        215    1762
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     151    1977 
   Size:        151    1826
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1702    1736    1977 
   Size:       1702      34     241

  Fragments arranged by size: 

                1702     241      34
 
BclI T'GATC_A

Cuts at:      0     998    1977 
   Size:        998     979
 
BglI GCCn_nnn'nGGC

Cuts at:      0     908    1977 
   Size:        908    1069
 
Bme1580I G_kGCm'C

Cuts at:      0     877    1574    1977 
   Size:        877     697     403

  Fragments arranged by size: 

                 877     697     403
 
BmgBI CAC'GTC

Cuts at:      0    1889    1977 
   Size:       1889      88
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     590     821    1535    1977 
   Size:        590     231     714     442

  Fragments arranged by size: 

                 714     590     442     231
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1917    1977 
   Size:       1917      60
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     406     512     601    1977 
   Size:        406     106      89    1376

  Fragments arranged by size: 

                1376     406     106      89
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     379    1780    1887    1977 
   Size:        379    1401     107      90

  Fragments arranged by size: 

                1401     379     107      90
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      28    1977 
   Size:         28    1949
 
BsaAI yAC'GTr

Cuts at:      0     708     927    1786    1977 
   Size:        708     219     859     191

  Fragments arranged by size: 

                 859     708     219     191
 
BsaBI GATnn'nnATC

Cuts at:      0    1661    1977 
   Size:       1661     316
 
BsaHI Gr'CG_yC

Cuts at:      0     212    1977 
   Size:        212    1765
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1717    1867    1870    1927    1977 
   Size:       1717     150       3      57      50

  Fragments arranged by size: 

                1717     150      57      50       3
 
BseYI C'CCAG_C

Cuts at:      0      19     262    1679    1977 
   Size:         19     243    1417     298

  Fragments arranged by size: 

                1417     298     243      19
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     913    1065    1977 
   Size:        913     152     912

  Fragments arranged by size: 

                 913     912     152
 
BsiHKAI G_wGCw'C

Cuts at:      0     877    1177    1265    1574    1977 
   Size:        877     300      88     309     403

  Fragments arranged by size: 

                 877     403     309     300      88
 
BsmI GAATG_Cn'

Cuts at:      0     717     851    1054    1453    1977 
   Size:        717     134     203     399     524

  Fragments arranged by size: 

                 717     524     399     203     134
 
Bsp1286I G_dGCh'C

Cuts at:      0     877    1177    1265    1574    1977 
   Size:        877     300      88     309     403

  Fragments arranged by size: 

                 877     403     309     300      88
 
BspHI T'CATG_A

Cuts at:      0    1169    1977 
   Size:       1169     808
 
BsrDI GCAATG_nn'

Cuts at:      0    1487    1977 
   Size:       1487     490
 
BsrGI T'GTAC_A

Cuts at:      0    1013    1659    1977 
   Size:       1013     646     318

  Fragments arranged by size: 

                1013     646     318
 
BssSI C'ACGA_G

Cuts at:      0    1902    1977 
   Size:       1902      75
 
BstEII G'GTnAC_C

Cuts at:      0    1617    1977 
   Size:       1617     360
 
BstYI r'GATC_y

Cuts at:      0     341    1977 
   Size:        341    1636
 
BtgI C'CryG_G

Cuts at:      0     823    1977 
   Size:        823    1154
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     151    1977 
   Size:        151    1826
 
ClaI AT'CG_AT

Cuts at:      0    1712    1977 
   Size:       1712     265
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0      35      70    1977 
   Size:         35      35    1907

  Fragments arranged by size: 

                1907      35      35
 
DraIII CAC_nnn'GTG

Cuts at:      0     252    1977 
   Size:        252    1725
 
DrdI GACnn_nn'nnGTC

Cuts at:      0      88    1977 
   Size:         88    1889
 
EaeI y'GGCC_r

Cuts at:      0       2     202     826    1977 
   Size:          2     200     624    1151

  Fragments arranged by size: 

                1151     624     200       2
 
EarI CTCTTCn'nnn_

Cuts at:      0     454    1216    1721    1977 
   Size:        454     762     505     256

  Fragments arranged by size: 

                 762     505     454     256
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     793    1977 
   Size:        793    1184
 
EcoICRI GAG'CTC

Cuts at:      0    1175    1263    1977 
   Size:       1175      88     714

  Fragments arranged by size: 

                1175     714      88
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     163     263     406     512     601     979    1977 
   Size:        163     100     143     106      89     378     998

  Fragments arranged by size: 

                 998     378     163     143     106     100      89
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1905    1977 
   Size:       1905      72
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1320    1834    1898    1977 
   Size:       1320     514      64      79

  Fragments arranged by size: 

                1320     514      79      64
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     531    1977 
   Size:        531    1446
 
HaeII r_GCGC'y

Cuts at:      0     215     900    1202    1977 
   Size:        215     685     302     775

  Fragments arranged by size: 

                 775     685     302     215
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     410     442    1340    1372    1977 
   Size:        410      32     898      32     605

  Fragments arranged by size: 

                 898     605     410      32      32
 
HindIII A'AGCT_T

Cuts at:      0    1802    1977 
   Size:       1802     175
 
KasI G'GCGC_C

Cuts at:      0     211    1977 
   Size:        211    1766
 
KpnI G_GTAC'C

Cuts at:      0    1042    1977 
   Size:       1042     935
 
MfeI C'AATT_G

Cuts at:      0    1397    1977 
   Size:       1397     580
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     657    1232    1452    1977 
   Size:        657     575     220     525

  Fragments arranged by size: 

                 657     575     525     220
 
MscI TGG'CCA

Cuts at:      0       4     828    1977 
   Size:          4     824    1149

  Fragments arranged by size: 

                1149     824       4
 
MslI CAynn'nnrTG

Cuts at:      0     303     457     628     985    1021    1105    1234    1495 
   Size:        303     154     171     357      36      84     129     261

Cuts at:   1495    1788    1977 
   Size:        293     189

  Fragments arranged by size: 

                 357     303     293     261     189     171     154     129
                  84      36
 
MspA1I CmG'CkG

Cuts at:      0    1405    1977 
   Size:       1405     572
 
NarI GG'CG_CC

Cuts at:      0     212    1977 
   Size:        212    1765
 
NcoI C'CATG_G

Cuts at:      0     823    1977 
   Size:        823    1154
 
NspI r_CATG'y

Cuts at:      0     921    1034    1977 
   Size:        921     113     943

  Fragments arranged by size: 

                 943     921     113
 
PasI CC'CwG_GG

Cuts at:      0    1623    1977 
   Size:       1623     354
 
PciI A'CATG_T

Cuts at:      0     917    1977 
   Size:        917    1060
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     252    1977 
   Size:        252    1725
 
PmlI CAC'GTG

Cuts at:      0     927    1786    1977 
   Size:        927     859     191

  Fragments arranged by size: 

                 927     859     191
 
PpuMI rG'GwC_Cy

Cuts at:      0    1320    1834    1977 
   Size:       1320     514     143

  Fragments arranged by size: 

                1320     514     143
 
PstI C_TGCA'G

Cuts at:      0     480    1977 
   Size:        480    1497
 
PvuII CAG'CTG

Cuts at:      0    1405    1977 
   Size:       1405     572
 
SacI G_AGCT'C

Cuts at:      0    1177    1265    1977 
   Size:       1177      88     712

  Fragments arranged by size: 

                1177     712      88
 
SanDI GG'GwC_CC

Cuts at:      0    1320    1834    1977 
   Size:       1320     514     143

  Fragments arranged by size: 

                1320     514     143
 
SfcI C'TryA_G

Cuts at:      0     161     476    1977 
   Size:        161     315    1501

  Fragments arranged by size: 

                1501     315     161
 
SfoI GGC'GCC

Cuts at:      0     213    1977 
   Size:        213    1764
 
SmlI C'TyrA_G

Cuts at:      0     394    1795    1866    1977 
   Size:        394    1401      71     111

  Fragments arranged by size: 

                1401     394     111      71
 
StuI AGG'CCT

Cuts at:      0    1139    1977 
   Size:       1139     838
 
StyI C'CwwG_G

Cuts at:      0     515     823    1125    1386    1847    1977 
   Size:        515     308     302     261     461     130

  Fragments arranged by size: 

                 515     461     308     302     261     130
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     326    1140    1977 
   Size:        326     814     837

  Fragments arranged by size: 

                 837     814     326
 
TatI w'GTAC_w

Cuts at:      0    1013    1580    1659    1696    1977 
   Size:       1013     567      79      37     281

  Fragments arranged by size: 

                1013     567     281      79      37
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     585     978    1107    1938    1977 
   Size:        585     393     129     831      39

  Fragments arranged by size: 

                 831     585     393     129      39
 

 Enzymes that do cut:

     AccI   Acc65I     AcuI     AfeI   AflIII     AhdI     AleI    AlwNI
    ApaLI     ApoI     AvaI     BaeI     BanI    BanII     BbeI     BbsI
     BcgI     BclI     BglI Bme1580I    BmgBI     BmrI     BplI     BpmI
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BseRI    BseYI     BsgI
  BsiHKAI     BsmI Bsp1286I    BspHI    BsrDI    BsrGI    BssSI   BstEII
    BstYI     BtgI    BtgZI     ClaI    CspCI   DraIII     DrdI     EaeI
     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I     FalI    HaeII
    Hin4I  HindIII     KasI     KpnI     MfeI     MmeI     MscI     MslI
   MspA1I     NarI     NcoI     NspI     PasI     PciI    PflMI     PmlI
    PpuMI     PstI    PvuII     SacI    SanDI     SfcI     SfoI     SmlI
     StuI     StyI    TaqII     TatI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AclI    AflII     AgeI     AlfI     AloI     ApaI
     AscI     AseI    AsiSI    AvrII    BamHI    BbvCI    BciVI    BfrBI
    BglII     BlpI     BmtI   Bpu10I    BsaWI    BsaXI    BsiEI    BsiWI
    BsmBI    BspEI    BspMI    BsrBI    BsrFI   BssHII   BstAPI    BstBI
    BstXI  BstZ17I   Bsu36I     BtsI     DraI     EagI    EcoRI    EcoRV
     FseI     FspI    FspAI   HincII     HpaI     MluI     NaeI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PfoI     PmeI
     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII
    SacII     SalI     SapI     SbfI     ScaI    SexAI     SfiI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI
     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com