Restriction map for pF1KE0325
##################################
# NO CUT ENZYMES (  126 enzymes) #
##################################

Acc65I      AccI        AccIII      AclI        AcsI        
AflII       AflIII      AgeI        AhdI        Alw44I      
Aor51HI     ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspI        
AviII       AvrII       BamHI       BciVI       BclI        
BfaI        BfrI        BglI        BglII       BlnI        
BlpI        Bpu1102I    BsaBI       BsaI        BsaWI       
BseAI       BsiEI       BsiWI       BsmBI       BsmI        
BspDI       BspEI       BspHI       BspLU11I    BsrBI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstZ17I     CelII       ClaI        
CpoI        DraI        DraIII      DrdI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      EcoNI       
EcoO65I     EcoRI       EcoRV       EcoT22I     FauI        
FbaI        FseI        FspI        HgaI        HindIII     
HpaI        KpnI        KspI        MaeI        MamI        
MfeI        MluI        MroI        MseI        MunI        
NdeI        NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmeI        PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacII       SalI        SapI        
SbfI        ScaI        SfiI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspI        StuI        SwaI        Tru9I       Tth111I     
Van91I      XbaI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

AatII        1087  GACGTC               GACGT^C
AlwNI        1180  CAGATGCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI          108  GGGCCC               GGGCC^C
AspHI         925  GAGCTC               G[AT]GC[AT]^C
AvaI          901  CCCGAG               C^[CT]CG[GA]G
BbeI            6  GGCGCC               GGCGC^C
BbsI          192  GAAGAC               GAAGAC(2/6)
BbvCI         222  CCTCAGC              CCTCAGC(-5/-2)
BcgI          105  CGAGGGCCCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI         192  GAAGAC               GAAGAC(2/6)
BseRI         535  GAGGAG               GAGGAG(10/8)
BsiHKAI       925  GAGCTC               G[AT]GC[AT]^C
BsmAI         586  GTCTC                GTCTC(1/5)
BsoBI         901  CCCGAG               C^[CT]CG[GA]G
BsrDI         227  GCAATG               GCAATG(2/0)
BsrFI        1493  GCCGGC               [GA]^CCGG[CT]
BstYI        1390  AGATCC               [GA]^GATC[CT]
Cfr10I       1493  GCCGGC               [GA]^CCGG[CT]
EarI         1012  CTCTTC               CTCTTC(1/4)
HaeII           6  GGCGCC               [GA]GCGC^[CT]
HincII        306  GTTGAC               GT[CT]^[GA]AC
HindII        306  GTTGAC               GT[CT]^[GA]AC
KasI            6  GGCGCC               G^GCGCC
Ksp632I      1012  CTCTTC               CTCTTC(1/4)
MflI         1390  AGATCC               [GA]^GATC[CT]
MslI           78  CACCCCCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI         1493  GCCGGC               GCC^GGC
NarI            6  GGCGCC               GG^CGCC
NgoMIV       1493  GCCGGC               G^CCGGC
NspI         1218  GCATGT               [GA]CATG^[CT]
SacI          925  GAGCTC               GAGCT^C
SexAI         572  ACCTGGT              A^CC[AT]GGT
SfoI            6  GGCGCC               GGC^GCC
TfiI         1361  GAATC                G^A[AT]TC
Tsp509I       684  AATT                 ^AATT
XcmI          866  CCATCCCCATCATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII        1390  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   31 enzymes) #
###################################

AccII        1108  CGCG                 CG^CG
             1168  CGCG                
AcyI            6  GGCGCC               G[GA]^CG[CT]C
             1087  GACGTC              
AlwI         1095  GATCC                GGATC(4/5)
             1391  GATCC               
BalI          272  TGGCCA               TGG^CCA
              440  TGGCCA              
BanII         108  GGGCCC               G[GA]GC[CT]^C
              925  GAGCTC              
BbrPI         709  CACGTG               CAC^GTG
              763  CACGTG              
BcnI           69  CCCGG                CC^[GC]GG
             1158  CCCGG               
BsaAI         709  CACGTG               [CT]AC^GT[GA]
              763  CACGTG              
BsaHI           6  GGCGCC               G[GA]^CG[CT]C
             1087  GACGTC              
Bsp1407I      482  TGTACA               T^GTACA
              986  TGTACA              
BsrGI         482  TGTACA               T^GTACA
              986  TGTACA              
BsrI          551  ACTGG                ACTGG(1/-1)
             1475  ACTGG               
BstUI        1108  CGCG                 CG^CG
             1168  CGCG                
BstXI        1277  CCAACATCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1400  CCAAAATCCTGG        
EcoT14I        83  CCATGG               C^C[AT][AT]GG
              269  CCATGG              
Hin1I           6  GGCGCC               G[GA]^CG[CT]C
             1087  GACGTC              
MaeIII        592  GTCAC                ^GT[ACGT]AC
             1129  GTCAC               
MluNI         272  TGGCCA               TGG^CCA
              440  TGGCCA              
MscI          272  TGGCCA               TGG^CCA
              440  TGGCCA              
MvnI         1108  CGCG                 CG^CG
             1168  CGCG                
NciI           69  CCCGG                CC^[GC]GG
             1158  CCCGG               
NcoI           83  CCATGG               C^CATGG
              269  CCATGG              
PleI          257  GACTC                GAGTC(4/5)
             1045  GAGTC               
PmaCI         709  CACGTG               CAC^GTG
              763  CACGTG              
PmlI          709  CACGTG               CAC^GTG
              763  CACGTG              
PpuMI         412  GGGACCC              [GA]G^G[AT]CC[CT]
              932  AGGACCC             
PshAI         309  GACAGCTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             1122  GACAGCAGTC          
PstI           13  CTGCAG               CTGCA^G
              793  CTGCAG              
SspBI         482  TGTACA               T^GTACA
              986  TGTACA              
StyI           83  CCATGG               C^C[AT][AT]GG
              269  CCATGG              
Tsp45I        592  GTCAC                ^GT[GC]AC
             1129  GTCAC               


#########################################
# 3 OR MORE CUT ENZYMES (   64 enzymes) #
#########################################

AciI        AfaI        AluI        AocI        AvaII       
BanI        BbvI        BmyI        BpmI        BsaJI       
BsgI        BsiYI       BslI        BsmFI       Bsp1286I    
BspMI       BssKI       BstF5I      BstNI       Bsu36I      
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        Eco81I      EcoO109I    
EcoRII      Fnu4HI      FokI        HaeIII      HapII       
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        MaeII       MboI        MboII       MnlI        
MspA1I      MspI        MvaI        MwoI        NdeII       
NlaIII      NlaIV       PvuII       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
TaqI        TseI        TspRI       TthHB8I     
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