Restriction map for pF1KE0364
##################################
# NO CUT ENZYMES (  106 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AflII       AgeI        AhdI        Alw44I      AocI        
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspI        AvrII       
BbeI        BcgI        BciVI       BclI        BfrI        
BlnI        BsaBI       BseAI       BsiWI       BsmBI       
Bsp1407I    BspDI       BspEI       BspHI       BsrGI       
BssHII      Bst1107I    BstBI       BstZ17I     Bsu36I      
ClaI        CpoI        DraI        DrdI        EagI        
Eam1105I    EclXI       Eco52I      Eco81I      EcoRI       
EcoRV       EcoT22I     FbaI        FseI        HindIII     
HinfI       KasI        KpnI        MamI        MfeI        
MluI        MroI        MslI        MunI        NarI        
NdeI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PinAI       PleI        
PmeI        PpuMI       PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsrII       SalI        SapI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
TfiI        Tsp509I     Tth111I     XbaI        XhoI        
XmnI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AccI         1291  GTCTAC               GT^[AC][GT]AC
AcyI         1897  GACGCC               G[GA]^CG[CT]C
Aor51HI      1262  AGCGCT               AGC^GCT
AviII        1559  TGCGCA               TGC^GCA
BanI          177  GGTGCC               G^G[CT][GA]CC
BbrPI        1012  CACGTG               CAC^GTG
BbsI         1932  GAAGAC               GAAGAC(2/6)
BbvCI         753  CCTCAGC              CCTCAGC(-5/-2)
BglII        1050  AGATCT               A^GATCT
BpuAI        1932  GAAGAC               GAAGAC(2/6)
BsaAI        1012  CACGTG               [CT]AC^GT[GA]
BsaHI        1897  GACGCC               G[GA]^CG[CT]C
BsaI         1249  GAGACC               GGTCTC(1/5)
BsaWI        1193  ACCGGA               [AT]^CCGG[AT]
BsiEI        1644  CGACCG               CG[GA][CT]^CG
BsmAI        1249  GAGAC                GTCTC(1/5)
BsmI         1376  GAATGC               GAATGC(1/-1)
BspLU11I      121  ACATGT               A^CATGT
BstAPI        642  GCACTCTGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI         598  CCAACAGCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EarI          871  CTCTTC               CTCTTC(1/4)
Eco47III     1262  AGCGCT               AGC^GCT
EcoNI         769  CCTACACCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI         1559  TGCGCA               TGC^GCA
HgaI         1897  GACGC                GACGC(5/10)
Hin1I        1897  GACGCC               G[GA]^CG[CT]C
HpaI         1738  GTTAAC               GTT^AAC
Ksp632I       871  CTCTTC               CTCTTC(1/4)
KspI         1524  CCGCGG               CCGC^GG
MseI         1739  TTAA                 T^TAA
NheI         1800  GCTAGC               G^CTAGC
NspI          121  ACATGT               [GA]CATG^[CT]
PflMI        1011  CCACGTGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI        1012  CACGTG               CAC^GTG
PmlI         1012  CACGTG               CAC^GTG
SacII        1524  CCGCGG               CCGC^GG
SmlI          321  CTTGAG               C^T[CT][GA]AG
Tru9I        1739  TTAA                 T^TAA
Van91I       1011  CCACGTGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AlwNI          77  CAGTCGCTG            CAG[ACGT][ACGT][ACGT]^CTG
              824  CAGTGCCTG           
BamHI         114  GGATCC               G^GATCC
             1034  GGATCC              
BfaI         1465  CTAG                 C^TAG
             1801  CTAG                
BpmI          703  CTGGAG               CTGGAG(16/14)
             2061  CTGGAG              
BseRI        1369  CTCCTC               GAGGAG(10/8)
              706  GAGGAG              
BsgI         1399  GTGCAG               GTGCAG(16/14)
             1795  GTGCAG              
BspMI         836  ACCTGC               ACCTGC(4/8)
             1716  GCAGGT              
BsrBI         361  GAGCGG               CCGCTC(-3/-3)
              928  GAGCGG              
BsrDI        1216  GCAATG               GCAATG(2/0)
             1223  GCAATG              
BssSI         371  CACGAG               CACGAG(-5/-1)
             1693  CACGAG              
BstEII        490  GGTGACC              G^GT[ACGT]ACC
             1908  GGTCACC             
BstPI         490  GGTGACC              G^GT[ACGT]ACC
             1908  GGTCACC             
EcoO65I       490  GGTGACC              G^GT[ACGT]ACC
             1908  GGTCACC             
MaeI         1465  CTAG                 C^TAG
             1801  CTAG                
PstI          724  CTGCAG               CTGCA^G
              937  CTGCAG              
PvuII         200  CAGCTG               CAG^CTG
              974  CAGCTG              
SacI          654  GAGCTC               GAGCT^C
             1150  GAGCTC              
SmaI          449  CCCGGG               CCC^GGG
              665  CCCGGG              
XmaCI         449  CCCGGG               C^CCGGG
              665  CCCGGG              
XmaI          449  CCCGGG               C^CCGGG
              665  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   93 enzymes) #
#########################################

AccII       AciI        AfaI        AflIII      AluI        
AlwI        ApaI        AspHI       AvaI        AvaII       
BalI        BanII       BbvI        BcnI        BglI        
BlpI        BmyI        Bpu1102I    BsaJI       BsiHKAI     
BsiYI       BslI        BsmFI       BsoBI       Bsp1286I    
BsrFI       BsrI        BssKI       BstF5I      BstNI       
BstUI       BstYI       Cac8I       CelII       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraII       
DraIII      DsaI        EaeI        EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HhaI        HinP1I      HincII      
HindII      HpaII       HphI        ItaI        MaeII       
MaeIII      MboI        MboII       MflI        MluNI       
MnlI        MscI        MspA1I      MspI        MvaI        
MvnI        MwoI        NaeI        NciI        NcoI        
NdeII       NgoMIV      NlaIII      NlaIV       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
StyI        TaqI        TseI        Tsp45I      TspRI       
TthHB8I     XcmI        XhoII       
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