Restriction map for pF1KE0364
 (Linear) (Six-base) MAPSORT of: KIEE0364.seq  Check: 6887  from: 1  to: 2070



KIEE0364.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:13  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1707    2070 
   Size:       1707     363
 
AccI GT'mk_AC

Cuts at:      0    1292    2070 
   Size:       1292     778
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     325     496    2070 
   Size:        325     171    1574

  Fragments arranged by size: 

                1574     325     171
 
AfeI AGC'GCT

Cuts at:      0    1264    2070 
   Size:       1264     806
 
AflIII A'CryG_T

Cuts at:      0     121    1682    1742    2070 
   Size:        121    1561      60     328

  Fragments arranged by size: 

                1561     328     121      60
 
AlwNI CAG_nnn'CTG

Cuts at:      0      82     829    2070 
   Size:         82     747    1241

  Fragments arranged by size: 

                1241     747      82
 
ApaI G_GGCC'C

Cuts at:      0     474     666    1350    2007    2070 
   Size:        474     192     684     657      63

  Fragments arranged by size: 

                 684     657     474     192      63
 
AvaI C'yCGr_G

Cuts at:      0     449     665     885    1532    1651    2070 
   Size:        449     216     220     647     119     419

  Fragments arranged by size: 

                 647     449     419     220     216     119
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1152    1185    2070 
   Size:       1152      33     885

  Fragments arranged by size: 

                1152     885      33
 
BamHI G'GATC_C

Cuts at:      0     114    1034    2070 
   Size:        114     920    1036

  Fragments arranged by size: 

                1036     920     114
 
BanI G'GyrC_C

Cuts at:      0     177    2070 
   Size:        177    1893
 
BanII G_rGCy'C

Cuts at:      0     474     658     666     789    1154    1350    2007    2070 
   Size:        474     184       8     123     365     196     657      63

  Fragments arranged by size: 

                 657     474     365     196     184     123      63       8
            
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1939    2070 
   Size:       1939     131
 
BbvCI CC'TCA_GC

Cuts at:      0     754    2070 
   Size:        754    1316
 
BglI GCCn_nnn'nGGC

Cuts at:      0      72     186     234     460     478    2070 
   Size:         72     114      48     226      18    1592

  Fragments arranged by size: 

                1592     226     114      72      48      18
 
BglII A'GATC_T

Cuts at:      0    1050    2070 
   Size:       1050    1020
 
BlpI GC'TnA_GC

Cuts at:      0     190     482     652    2070 
   Size:        190     292     170    1418

  Fragments arranged by size: 

                1418     292     190     170
 
Bme1580I G_kGCm'C

Cuts at:      0     182     393     474     666    1350    2007    2070 
   Size:        182     211      81     192     684     657      63

  Fragments arranged by size: 

                 684     657     211     192     182      81      63
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     817    2001    2070 
   Size:        817    1184      69

  Fragments arranged by size: 

                1184     817      69
 
BmtI G_CTAG'C

Cuts at:      0    1804    2070 
   Size:       1804     266
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     724    2070 
   Size:        724    1346
 
Bpu10I CC'TnA_GC

Cuts at:      0     754    2070 
   Size:        754    1316
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     342    2070 
   Size:        342    1728
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1243    2070 
   Size:       1243     827
 
BsaAI yAC'GTr

Cuts at:      0    1014    2070 
   Size:       1014    1056
 
BsaHI Gr'CG_yC

Cuts at:      0    1898    2070 
   Size:       1898     172
 
BsaWI w'CCGG_w

Cuts at:      0    1193    2070 
   Size:       1193     877
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     206     236    1693    1723    1819    1849    2070 
   Size:        206      30    1457      30      96      30     221

  Fragments arranged by size: 

                1457     221     206      96      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     721    1360    2070 
   Size:        721     639     710

  Fragments arranged by size: 

                 721     710     639
 
BseYI C'CCAG_C

Cuts at:      0     486     573     588     844    1178    2070 
   Size:        486      87      15     256     334     892

  Fragments arranged by size: 

                 892     486     334     256      87      15
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1420    1816    2070 
   Size:       1420     396     254

  Fragments arranged by size: 

                1420     396     254
 
BsiEI CG_ry'CG

Cuts at:      0    1647    2070 
   Size:       1647     423
 
BsiHKAI G_wGCw'C

Cuts at:      0     372     658    1154    2070 
   Size:        372     286     496     916

  Fragments arranged by size: 

                 916     496     372     286
 
BsmI GAATG_Cn'

Cuts at:      0    1382    2070 
   Size:       1382     688
 
Bsp1286I G_dGCh'C

Cuts at:      0     182     372     393     474     658     666     789    1154 
   Size:        182     190      21      81     184       8     123     365

Cuts at:   1154    1350    2007    2070 
   Size:        196     657      63

  Fragments arranged by size: 

                 657     365     196     190     184     182     123      81
                  63      21       8
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     845    1707    2070 
   Size:        845     862     363

  Fragments arranged by size: 

                 862     845     363
 
BsrBI CCG'CTC

Cuts at:      0     363     930    2070 
   Size:        363     567    1140

  Fragments arranged by size: 

                1140     567     363
 
BsrDI GCAATG_nn'

Cuts at:      0    1223    1230    2070 
   Size:       1223       7     840

  Fragments arranged by size: 

                1223     840       7
 
BsrFI r'CCGG_y

Cuts at:      0     261     326     418    2070 
   Size:        261      65      92    1652

  Fragments arranged by size: 

                1652     261      92      65
 
BssSI C'ACGA_G

Cuts at:      0     371    1693    2070 
   Size:        371    1322     377

  Fragments arranged by size: 

                1322     377     371
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     648    2070 
   Size:        648    1422
 
BstEII G'GTnAC_C

Cuts at:      0     490    1908    2070 
   Size:        490    1418     162

  Fragments arranged by size: 

                1418     490     162
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     605    2070 
   Size:        605    1465
 
BstYI r'GATC_y

Cuts at:      0     114     287     710    1034    1050    1280    2070 
   Size:        114     173     423     324      16     230     790

  Fragments arranged by size: 

                 790     423     324     230     173     114      16
 
BtgI C'CryG_G

Cuts at:      0     183    1524    1613    1901    2070 
   Size:        183    1341      89     288     169

  Fragments arranged by size: 

                1341     288     183     169      89
 
DraIII CAC_nnn'GTG

Cuts at:      0     648     825    1698    2070 
   Size:        648     177     873     372

  Fragments arranged by size: 

                 873     648     372     177
 
EaeI y'GGCC_r

Cuts at:      0      73     143     281     581     621    1610    1809    2070 
   Size:         73      70     138     300      40     989     199     261

  Fragments arranged by size: 

                 989     300     261     199     138      73      70      40
            
 
EarI CTCTTCn'nnn_

Cuts at:      0     877    2070 
   Size:        877    1193
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1198    1435    2070 
   Size:       1198     237     635

  Fragments arranged by size: 

                1198     635     237
 
EcoICRI GAG'CTC

Cuts at:      0     656    1152    2070 
   Size:        656     496     918

  Fragments arranged by size: 

                 918     656     496
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     325     496     724    2070 
   Size:        325     171     228    1346

  Fragments arranged by size: 

                1346     325     228     171
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     773    2070 
   Size:        773    1297
 
EcoO109I rG'GnC_Cy

Cuts at:      0      54     409     471     662    1346    1347    2070 
   Size:         54     355      62     191     684       1     723

  Fragments arranged by size: 

                 723     684     355     191      62      54       1
 
FspI TGC'GCA

Cuts at:      0    1561    2070 
   Size:       1561     509
 
HaeII r_GCGC'y

Cuts at:      0     814    1266    1341    2070 
   Size:        814     452      75     729

  Fragments arranged by size: 

                 814     729     452      75
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1422    1454    1669    1701    2070 
   Size:       1422      32     215      32     369

  Fragments arranged by size: 

                1422     369     215      32      32
 
HincII GTy'rAC

Cuts at:      0    1326    1740    1977    2070 
   Size:       1326     414     237      93

  Fragments arranged by size: 

                1326     414     237      93
 
HpaI GTT'AAC

Cuts at:      0    1740    2070 
   Size:       1740     330
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1523    2070 
   Size:       1523     547
 
MscI TGG'CCA

Cuts at:      0      75     145     283     583    1612    2070 
   Size:         75      70     138     300    1029     458

  Fragments arranged by size: 

                1029     458     300     138      75      70
 
MspA1I CmG'CkG

Cuts at:      0     202     743     976    1526    2070 
   Size:        202     541     233     550     544

  Fragments arranged by size: 

                 550     544     541     233     202
 
NaeI GCC'GGC

Cuts at:      0     263     328     420    2070 
   Size:        263      65      92    1650

  Fragments arranged by size: 

                1650     263      92      65
 
NcoI C'CATG_G

Cuts at:      0     183    1613    1901    2070 
   Size:        183    1430     288     169

  Fragments arranged by size: 

                1430     288     183     169
 
NgoMIV G'CCGG_C

Cuts at:      0     261     326     418    2070 
   Size:        261      65      92    1652

  Fragments arranged by size: 

                1652     261      92      65
 
NheI G'CTAG_C

Cuts at:      0    1800    2070 
   Size:       1800     270
 
NspI r_CATG'y

Cuts at:      0     125    2070 
   Size:        125    1945
 
PasI CC'CwG_GG

Cuts at:      0     173    1350    2070 
   Size:        173    1177     720

  Fragments arranged by size: 

                1177     720     173
 
PciI A'CATG_T

Cuts at:      0     121    2070 
   Size:        121    1949
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1017    2070 
   Size:       1017    1053
 
PfoI T'CCnGG_A

Cuts at:      0     692    1892    2070 
   Size:        692    1200     178

  Fragments arranged by size: 

                1200     692     178
 
PmlI CAC'GTG

Cuts at:      0    1014    2070 
   Size:       1014    1056
 
PspOMI G'GGCC_C

Cuts at:      0     470     662    1346    2003    2070 
   Size:        470     192     684     657      67

  Fragments arranged by size: 

                 684     657     470     192      67
 
PstI C_TGCA'G

Cuts at:      0     728     941    2070 
   Size:        728     213    1129

  Fragments arranged by size: 

                1129     728     213
 
PvuII CAG'CTG

Cuts at:      0     202     976    2070 
   Size:        202     774    1094

  Fragments arranged by size: 

                1094     774     202
 
SacI G_AGCT'C

Cuts at:      0     658    1154    2070 
   Size:        658     496     916

  Fragments arranged by size: 

                 916     658     496
 
SacII CC_GC'GG

Cuts at:      0    1527    2070 
   Size:       1527     543
 
SfcI C'TryA_G

Cuts at:      0     724     937    1457    2070 
   Size:        724     213     520     613

  Fragments arranged by size: 

                 724     613     520     213
 
SmaI CCC'GGG

Cuts at:      0     451     667    2070 
   Size:        451     216    1403

  Fragments arranged by size: 

                1403     451     216
 
SmlI C'TyrA_G

Cuts at:      0     321    2070 
   Size:        321    1749
 
SrfI GCCC'GGGC

Cuts at:      0     451    2070 
   Size:        451    1619
 
StyI C'CwwG_G

Cuts at:      0     183    1490    1613    1901    2070 
   Size:        183    1307     123     288     169

  Fragments arranged by size: 

                1307     288     183     169     123
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     165     478     836    1258    2070 
   Size:        165     313     358     422     812

  Fragments arranged by size: 

                 812     422     358     313     165
 
TatI w'GTAC_w

Cuts at:      0    1989    2070 
   Size:       1989      81
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     206     238    1190    1222    2070 
   Size:        206      32     952      32     848

  Fragments arranged by size: 

                 952     848     206      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     251    1068    1695    2070 
   Size:        251     817     627     375

  Fragments arranged by size: 

                 817     627     375     251
 
XmaI C'CCGG_G

Cuts at:      0     449     665    2070 
   Size:        449     216    1405

  Fragments arranged by size: 

                1405     449     216
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AfeI   AflIII    AlwNI     ApaI     AvaI
     BaeI    BamHI     BanI    BanII     BbsI    BbvCI     BglI    BglII
     BlpI Bme1580I     BmrI     BmtI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI     BsmI Bsp1286I    BspMI    BsrBI    BsrDI    BsrFI    BssSI
   BstAPI   BstEII    BstXI    BstYI     BtgI   DraIII     EaeI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I     FspI    HaeII    Hin4I
   HincII     HpaI     MmeI     MscI   MspA1I     NaeI     NcoI   NgoMIV
     NheI     NspI     PasI     PciI    PflMI     PfoI     PmlI   PspOMI
     PstI    PvuII     SacI    SacII     SfcI     SmaI     SmlI     SrfI
     StyI    TaqII     TatI     TstI     XcmI     XmaI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI    AflII     AgeI     AhdI     AleI     AlfI
     AloI    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII     BbeI
     BcgI    BciVI     BclI    BfrBI    BmgBI     BplI    BsaBI    BsiWI
    BsmBI    BspEI    BspHI    BsrGI   BssHII    BstBI  BstZ17I   Bsu36I
    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI     EagI    EcoRI
    EcoRV     FalI     FseI    FspAI  HindIII     KasI     KpnI     MfeI
     MluI     MslI     NarI     NdeI     NotI     NruI     NsiI     PacI
     PmeI     PpiI    PpuMI    PshAI     PsiI    PspXI     PsrI     PvuI
    RsrII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI    SnaBI     SpeI     SphI     SspI     StuI
     SwaI  Tth111I     XbaI     XhoI     XmnI     ZraI
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