Restriction map for pF1KB6408
##################################
# NO CUT ENZYMES (  149 enzymes) #
##################################

AatII       AccI        AccII       AccIII      AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      AocI        ApaI        ApaLI       AscI        
AseI        AsnI        Asp700I     AspEI       AspHI       
AspI        AvaI        AviII       AvrII       BalI        
BbeI        BbrPI       BbsI        BbvCI       BcgI        
BclI        BfrI        BglI        BglII       BlnI        
BlpI        Bpu1102I    BpuAI       BsaAI       BsaHI       
BsaWI       BseAI       BsiEI       BsiHKAI     BsiWI       
BsmBI       BsmI        BsoBI       Bsp1407I    BspDI       
BspEI       BspLU11I    BsrBI       BsrDI       BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstBI       
BstEII      BstPI       BstUI       BstXI       BstZ17I     
Bsu36I      CelII       Cfr10I      ClaI        CpoI        
DraIII      DrdI        EaeI        EagI        Eam1105I    
EclXI       Eco52I      Eco81I      EcoNI       EcoO65I     
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
FspI        HgaI        Hin1I       HincII      HindII      
HindIII     HpaI        KasI        KspI        MfeI        
MluI        MluNI       MroI        MscI        MunI        
MvnI        NaeI        NarI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        Sse8387I    SspBI       SspI        StuI        
SwaI        Tth111I     Van91I      XbaI        XcmI        
XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

Acc65I        350  GGTACC               G^GTACC
AciI          538  GCGG                 CCGC(-3/-1)
AcsI          963  AAATTC               [GA]^AATT[CT]
AfaI          351  GTAC                 GT^AC
Aor51HI       386  AGCGCT               AGC^GCT
ApoI          963  AAATTC               [GA]^AATT[CT]
Asp718I       350  GGTACC               G^GTACC
BamHI           3  GGATCC               G^GATCC
BanII          13  GGGCTC               G[GA]GC[CT]^C
BciVI         317  GGATAC               GTATCC(6/5)
BcnI          676  CCCGG                CC^[GC]GG
BsaBI         750  GATTCTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI          420  GGTCTC               GGTCTC(1/5)
BseRI          16  CTCCTC               GAGGAG(10/8)
BspHI         746  TCATGA               T^CATGA
BstAPI        715  GCAGTGTATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI           3  GGATCC               [GA]^GATC[CT]
DraI          444  TTTAAA               TTT^AAA
Eco47III      386  AGCGCT               AGC^GCT
FauI          538  GCGGG                CCCGC(4/6)
HaeII         386  AGCGCT               [GA]GCGC^[CT]
KpnI          350  GGTACC               GGTAC^C
MaeII         267  ACGT                 A^CGT
MamI          750  GATTCTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI            3  GGATCC               [GA]^GATC[CT]
MslI          756  CATCATGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        522  CAGCTG               C[AC]G^C[GT]G
NciI          676  CCCGG                CC^[GC]GG
NcoI          709  CCATGG               C^CATGG
NdeI          143  CATATG               CA^TATG
NspI          603  GCATGC               [GA]CATG^[CT]
PstI          397  CTGCAG               CTGCA^G
PvuII         522  CAGCTG               CAG^CTG
RcaI          746  TCATGA               T^CATGA
RsaI          351  GTAC                 GT^AC
SexAI         858  ACCAGGT              A^CC[AT]GGT
SphI          603  GCATGC               GCATG^C
TaqI          221  TCGA                 T^CGA
TthHB8I       221  TCGA                 T^CGA
XhoII           3  GGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AlwNI         932  CAGAGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
              944  CAGAGTCTG           
BanI          350  GGTACC               G^G[CT][GA]CC
              541  GGCACC              
BfaI          274  CTAG                 C^TAG
              983  CTAG                
BmyI           13  GGGCTC               G[AGT]GC[ACT]^C
              540  GGGCAC              
BsmAI         237  GTCTC                GTCTC(1/5)
              421  GTCTC               
Bsp1286I       13  GGGCTC               G[AGT]GC[ACT]^C
              540  GGGCAC              
BspMI         469  ACCTGC               ACCTGC(4/8)
              417  GCAGGT              
BsrI          207  ACTGG                ACTGG(1/-1)
              483  ACTGG               
CfoI           81  GCGC                 GCG^C
              387  GCGC                
DdeI          696  CTCAG                C^T[ACGT]AG
              896  CTCAG               
DsaI          670  CCGTGG               C^C[GA][CT]GG
              709  CCATGG              
EarI          866  CTCTTC               CTCTTC(1/4)
              785  GAAGAG              
EcoT14I         8  CCAAGG               C^C[AT][AT]GG
              709  CCATGG              
HapII         108  CCGG                 C^CGG
              677  CCGG                
HhaI           81  GCGC                 GCG^C
              387  GCGC                
HinP1I         81  GCGC                 G^CGC
              387  GCGC                
HpaII         108  CCGG                 C^CGG
              677  CCGG                
HphI          308  TCACC                GGTGA(8/7)
              587  TCACC               
Ksp632I       866  CTCTTC               CTCTTC(1/4)
              785  GAAGAG              
MaeI          274  CTAG                 C^TAG
              983  CTAG                
MaeIII        190  GTCAC                ^GT[ACGT]AC
              988  GTGAC               
MseI          437  TTAA                 T^TAA
              445  TTAA                
MspI          108  CCGG                 C^CGG
              677  CCGG                
PleI          883  GACTC                GAGTC(4/5)
              946  GAGTC               
SfaNI         680  GATGC                GCATC(5/9)
              176  GCATC               
SfcI          397  CTGCAG               C^T[GA][CT]AG
              772  CTACAG              
StyI            8  CCAAGG               C^C[AT][AT]GG
              709  CCATGG              
Tru9I         437  TTAA                 T^TAA
              445  TTAA                
Tsp45I        190  GTCAC                ^GT[GC]AC
              988  GTGAC               


#########################################
# 3 OR MORE CUT ENZYMES (   40 enzymes) #
#########################################

AluI        AlwI        AvaII       BbvI        BpmI        
BsaJI       BsgI        BsiYI       BslI        BsmFI       
BssKI       BstF5I      BstNI       Cac8I       Cfr13I      
DpnII       DraII       EcoO109I    EcoRII      Fnu4HI      
FokI        HaeIII      HinfI       ItaI        MboI        
MboII       MnlI        MvaI        MwoI        NdeII       
NlaIII      NlaIV       PpuMI       Sau3AI      Sau96I      
ScrFI       TfiI        TseI        Tsp509I     TspRI       
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