Restriction map for pF1KB6408
 (Linear) (Six-base) MAPSORT of: KIBB6408.seq  Check: 1176  from: 1  to: 1005



KIBB6408.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:46  ..

Acc65I G'GTAC_C

Cuts at:      0     350    1005 
   Size:        350     655
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     648     853    1005 
   Size:        648     205     152

  Fragments arranged by size: 

                 648     205     152
 
AfeI AGC'GCT

Cuts at:      0     388    1005 
   Size:        388     617
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     145    1005 
   Size:        145     860
 
AlwNI CAG_nnn'CTG

Cuts at:      0     937     949    1005 
   Size:        937      12      56

  Fragments arranged by size: 

                 937      56      12
 
ApoI r'AATT_y

Cuts at:      0     963    1005 
   Size:        963      42
 
BamHI G'GATC_C

Cuts at:      0       3    1005 
   Size:          3    1002
 
BanI G'GyrC_C

Cuts at:      0     350     541    1005 
   Size:        350     191     464

  Fragments arranged by size: 

                 464     350     191
 
BanII G_rGCy'C

Cuts at:      0      17    1005 
   Size:         17     988
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     311    1005 
   Size:        311     694
 
Bme1580I G_kGCm'C

Cuts at:      0     544    1005 
   Size:        544     461
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     493    1005 
   Size:        493     512
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     438    1005 
   Size:        438     567
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     229     319     385     413     546    1005 
   Size:        229      90      66      28     133     459

  Fragments arranged by size: 

                 459     229     133      90      66      28
 
Bpu10I CC'TnA_GC

Cuts at:      0     896    1005 
   Size:        896     109
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     426    1005 
   Size:        426     579
 
BsaBI GATnn'nnATC

Cuts at:      0     754    1005 
   Size:        754     251
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0       7    1005 
   Size:          7     998
 
BseYI C'CCAG_C

Cuts at:      0     558     599     724     891    1005 
   Size:        558      41     125     167     114

  Fragments arranged by size: 

                 558     167     125     114      41
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     456     474     609    1005 
   Size:        456      18     135     396

  Fragments arranged by size: 

                 456     396     135      18
 
Bsp1286I G_dGCh'C

Cuts at:      0      17     544    1005 
   Size:         17     527     461

  Fragments arranged by size: 

                 527     461      17
 
BspHI T'CATG_A

Cuts at:      0     746    1005 
   Size:        746     259
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     408     478    1005 
   Size:        408      70     527

  Fragments arranged by size: 

                 527     408      70
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     721    1005 
   Size:        721     284
 
BstYI r'GATC_y

Cuts at:      0       3    1005 
   Size:          3    1002
 
BtgI C'CryG_G

Cuts at:      0     670     709    1005 
   Size:        670      39     296

  Fragments arranged by size: 

                 670     296      39
 
BtsI GCAGTG_nn'

Cuts at:      0     722    1005 
   Size:        722     283
 
DraI TTT'AAA

Cuts at:      0     446    1005 
   Size:        446     559
 
EarI CTCTTCn'nnn_

Cuts at:      0     780     872    1005 
   Size:        780      92     133

  Fragments arranged by size: 

                 780     133      92
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     229     319     385     413     546     648     853    1005 
   Size:        229      90      66      28     133     102     205     152

  Fragments arranged by size: 

                 229     205     152     133     102      90      66      28
            
 
EcoO109I rG'GnC_Cy

Cuts at:      0     259     467     862     900    1005 
   Size:        259     208     395      38     105

  Fragments arranged by size: 

                 395     259     208     105      38
 
HaeII r_GCGC'y

Cuts at:      0     390    1005 
   Size:        390     615
 
KpnI G_GTAC'C

Cuts at:      0     354    1005 
   Size:        354     651
 
MslI CAynn'nnrTG

Cuts at:      0     760    1005 
   Size:        760     245
 
MspA1I CmG'CkG

Cuts at:      0     524    1005 
   Size:        524     481
 
NcoI C'CATG_G

Cuts at:      0     709    1005 
   Size:        709     296
 
NdeI CA'TA_TG

Cuts at:      0     144    1005 
   Size:        144     861
 
NspI r_CATG'y

Cuts at:      0     607    1005 
   Size:        607     398
 
PasI CC'CwG_GG

Cuts at:      0     312    1005 
   Size:        312     693
 
PfoI T'CCnGG_A

Cuts at:      0     953    1005 
   Size:        953      52
 
PpuMI rG'GwC_Cy

Cuts at:      0     467     862     900    1005 
   Size:        467     395      38     105

  Fragments arranged by size: 

                 467     395     105      38
 
PstI C_TGCA'G

Cuts at:      0     401    1005 
   Size:        401     604
 
PvuII CAG'CTG

Cuts at:      0     524    1005 
   Size:        524     481
 
SexAI A'CCwGG_T

Cuts at:      0     858    1005 
   Size:        858     147
 
SfcI C'TryA_G

Cuts at:      0     397     772    1005 
   Size:        397     375     233

  Fragments arranged by size: 

                 397     375     233
 
SphI G_CATG'C

Cuts at:      0     607    1005 
   Size:        607     398
 
StyI C'CwwG_G

Cuts at:      0       8     709    1005 
   Size:          8     701     296

  Fragments arranged by size: 

                 701     296       8
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     559     563    1005 
   Size:        559       4     442

  Fragments arranged by size: 

                 559     442       4
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     110     142     565     597    1005 
   Size:        110      32     423      32     408

  Fragments arranged by size: 

                 423     408     110      32      32
 

 Enzymes that do cut:

   Acc65I     AcuI     AfeI     AlfI    AlwNI     ApoI    BamHI     BanI
    BanII    BciVI Bme1580I     BmrI     BplI     BpmI   Bpu10I     BsaI
    BsaBI    BseRI    BseYI     BsgI Bsp1286I    BspHI    BspMI   BstAPI
    BstYI     BtgI     BtsI     DraI     EarI  Eco57MI EcoO109I    HaeII
     KpnI     MslI   MspA1I     NcoI     NdeI     NspI     PasI     PfoI
    PpuMI     PstI    PvuII    SexAI     SfcI     SphI     StyI    TaqII
     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI    AflII   AflIII     AgeI     AhdI
     AleI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI     BbeI     BbsI    BbvCI     BcgI     BclI    BfrBI
     BglI    BglII     BlpI    BmgBI     BmtI    BpuEI    BsaAI    BsaHI
    BsaWI    BsaXI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI   DraIII     DrdI
     EaeI     EagI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    Hin4I   HincII  HindIII     HpaI     KasI
     MfeI     MluI     MmeI     MscI     NaeI     NarI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PciI    PflMI     PmeI     PmlI
     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SrfI
     SspI     StuI     SwaI     TatI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com