Restriction map for pF1KB6524
 (Linear) (Six-base) MAPSORT of: KIBB6524.seq  Check: 7873  from: 1  to: 1161



KIBB6524.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:47  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     465    1161 
   Size:        465     696
 
AccI GT'mk_AC

Cuts at:      0     587    1161 
   Size:        587     574
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     493     649     979    1161 
   Size:        493     156     330     182

  Fragments arranged by size: 

                 493     330     182     156
 
AleI CACnn'nnGTG

Cuts at:      0    1100    1161 
   Size:       1100      61
 
AlwNI CAG_nnn'CTG

Cuts at:      0     357    1161 
   Size:        357     804
 
ApaLI G'TGCA_C

Cuts at:      0     109    1093    1161 
   Size:        109     984      68

  Fragments arranged by size: 

                 984     109      68
 
BamHI G'GATC_C

Cuts at:      0     800    1161 
   Size:        800     361
 
BanI G'GyrC_C

Cuts at:      0     708     780     885    1056    1137    1161 
   Size:        708      72     105     171      81      24

  Fragments arranged by size: 

                 708     171     105      81      72      24
 
BanII G_rGCy'C

Cuts at:      0     299     500    1161 
   Size:        299     201     661

  Fragments arranged by size: 

                 661     299     201
 
BglI GCCn_nnn'nGGC

Cuts at:      0     222    1161 
   Size:        222     939
 
Bme1580I G_kGCm'C

Cuts at:      0     113     669     785     890    1061    1097    1142    1161 
   Size:        113     556     116     105     171      36      45      19

  Fragments arranged by size: 

                 556     171     116     113     105      45      36      19
            
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     181     283     385     826    1161 
   Size:        181     102     102     441     335

  Fragments arranged by size: 

                 441     335     181     102     102
 
Bpu10I CC'TnA_GC

Cuts at:      0     146    1161 
   Size:        146    1015
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     959    1161 
   Size:        959     202
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      14      19     451    1039    1161 
   Size:         14       5     432     588     122

  Fragments arranged by size: 

                 588     432     122      14       5
 
BseYI C'CCAG_C

Cuts at:      0      51      68     236    1161 
   Size:         51      17     168     925

  Fragments arranged by size: 

                 925     168      51      17
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     987    1161 
   Size:        987     174
 
BsiHKAI G_wGCw'C

Cuts at:      0     113    1097    1161 
   Size:        113     984      64

  Fragments arranged by size: 

                 984     113      64
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     121    1161 
   Size:        121    1040
 
Bsp1286I G_dGCh'C

Cuts at:      0     113     299     500     669     785     890    1061    1097 
   Size:        113     186     201     169     116     105     171      36

Cuts at:   1097    1142    1161 
   Size:         45      19

  Fragments arranged by size: 

                 201     186     171     169     116     113     105      45
                  36      19
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     221     465    1161 
   Size:        221     244     696

  Fragments arranged by size: 

                 696     244     221
 
BsrBI CCG'CTC

Cuts at:      0    1107    1161 
   Size:       1107      54
 
BsrDI GCAATG_nn'

Cuts at:      0     741    1161 
   Size:        741     420
 
BsrFI r'CCGG_y

Cuts at:      0     941    1161 
   Size:        941     220
 
BssSI C'ACGA_G

Cuts at:      0      31    1161 
   Size:         31    1130
 
BstEII G'GTnAC_C

Cuts at:      0     873    1161 
   Size:        873     288
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     256    1161 
   Size:        256     905
 
BstYI r'GATC_y

Cuts at:      0     800    1161 
   Size:        800     361
 
BstZ17I GTA'TAC

Cuts at:      0     588    1161 
   Size:        588     573
 
BtgI C'CryG_G

Cuts at:      0     540    1011    1161 
   Size:        540     471     150

  Fragments arranged by size: 

                 540     471     150
 
BtsI GCAGTG_nn'

Cuts at:      0     999    1161 
   Size:        999     162
 
EaeI y'GGCC_r

Cuts at:      0    1043    1063    1161 
   Size:       1043      20      98

  Fragments arranged by size: 

                1043      98      20
 
EarI CTCTTCn'nnn_

Cuts at:      0      19     955    1161 
   Size:         19     936     206

  Fragments arranged by size: 

                 936     206      19
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     181     283     385     493     649     826     979    1161 
   Size:        181     102     102     108     156     177     153     182

  Fragments arranged by size: 

                 182     181     177     156     153     108     102     102
            
 
EcoO109I rG'GnC_Cy

Cuts at:      0     375     385     840    1161 
   Size:        375      10     455     321

  Fragments arranged by size: 

                 455     375     321      10
 
FspI TGC'GCA

Cuts at:      0     421    1161 
   Size:        421     740
 
HaeII r_GCGC'y

Cuts at:      0    1133    1161 
   Size:       1133      28
 
HincII GTy'rAC

Cuts at:      0     726    1161 
   Size:        726     435
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1004    1161 
   Size:       1004     157
 
MslI CAynn'nnrTG

Cuts at:      0     595     988    1100    1161 
   Size:        595     393     112      61

  Fragments arranged by size: 

                 595     393     112      61
 
MspA1I CmG'CkG

Cuts at:      0     169     513     662    1161 
   Size:        169     344     149     499

  Fragments arranged by size: 

                 499     344     169     149
 
NaeI GCC'GGC

Cuts at:      0     943    1161 
   Size:        943     218
 
NgoMIV G'CCGG_C

Cuts at:      0     941    1161 
   Size:        941     220
 
NspI r_CATG'y

Cuts at:      0     154    1161 
   Size:        154    1007
 
PasI CC'CwG_GG

Cuts at:      0     669    1161 
   Size:        669     492
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     796    1161 
   Size:        796     365
 
PfoI T'CCnGG_A

Cuts at:      0     803    1161 
   Size:        803     358
 
PpuMI rG'GwC_Cy

Cuts at:      0     375    1161 
   Size:        375     786
 
PstI C_TGCA'G

Cuts at:      0     774    1161 
   Size:        774     387
 
PvuII CAG'CTG

Cuts at:      0     513    1161 
   Size:        513     648
 
SanDI GG'GwC_CC

Cuts at:      0     375    1161 
   Size:        375     786
 
SfcI C'TryA_G

Cuts at:      0     770    1161 
   Size:        770     391
 
StyI C'CwwG_G

Cuts at:      0     116     479     533     704    1161 
   Size:        116     363      54     171     457

  Fragments arranged by size: 

                 457     363     171     116      54
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     101     221     250     846    1161 
   Size:        101     120      29     596     315

  Fragments arranged by size: 

                 596     315     120     101      29
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     155     187     701     733    1161 
   Size:        155      32     514      32     428

  Fragments arranged by size: 

                 514     428     155      32      32
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AleI    AlwNI    ApaLI    BamHI     BanI
    BanII     BglI Bme1580I     BpmI   Bpu10I     BsaI    BseRI    BseYI
     BsgI  BsiHKAI    BsmBI Bsp1286I    BspMI    BsrBI    BsrDI    BsrFI
    BssSI   BstEII    BstXI    BstYI  BstZ17I     BtgI     BtsI     EaeI
     EarI  Eco57MI EcoO109I     FspI    HaeII   HincII     MmeI     MslI
   MspA1I     NaeI   NgoMIV     NspI     PasI    PflMI     PfoI    PpuMI
     PstI    PvuII    SanDI     SfcI     StyI    TaqII     TstI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AlfI     AloI     ApaI     ApoI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI
    BfrBI    BglII     BlpI    BmgBI     BmrI     BmtI     BplI    BpuEI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BsiEI    BsiWI     BsmI
    BspEI    BspHI    BsrGI   BssHII   BstAPI    BstBI   Bsu36I    BtgZI
     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EciI  EcoICRI
    EcoNI    EcoRI    EcoRV     FalI     FseI    FspAI    Hin4I  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MscI     NarI     NcoI
     NdeI     NheI     NotI     NruI     NsiI     PacI     PciI     PmeI
     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI
    RsrII     SacI    SacII     SalI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI     TatI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI
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