Restriction map for pF1KE0041
##################################
# NO CUT ENZYMES (  147 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AgeI        AhdI        
Alw44I      AocI        Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AvaII       AviII       AvrII       
BalI        BamHI       BbeI        BbrPI       BbvCI       
BcgI        BciVI       BclI        BfrI        BglI        
BglII       BlnI        BlpI        Bpu1102I    BsaHI       
BsaI        BsaWI       BseAI       BsgI        BsiEI       
BsiHKAI     BsiWI       BsmAI       BsmBI       BsmI        
Bsp1407I    BspDI       BspEI       BsrDI       BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstEII      BstPI       BstUI       BstXI       BstYI       
BstZ17I     Bsu36I      CelII       Cfr10I      ClaI        
CpoI        DraII       DraIII      DrdI        DsaI        
EaeI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoO109I    EcoO65I     EcoRV       
FauI        FbaI        FseI        FspI        HaeII       
HgaI        Hin1I       HincII      HindII      HindIII     
HpaI        HphI        KasI        KpnI        KspI        
MfeI        MflI        MluI        MluNI       MroI        
MscI        MunI        MvnI        NaeI        NarI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshBI       Psp1406I    PvuI        RsrII       
SacI        SacII       SalI        SbfI        ScaI        
SfiI        SfoI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       StuI        
SwaI        Tsp45I      XbaI        XhoII       XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AciI           98  CCGC                 CCGC(-3/-1)
AlwNI         130  CAGCAACTG            CAG[ACGT][ACGT][ACGT]^CTG
AspI          429  GACGAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          263  CTCGAG               C^[CT]CG[GA]G
BanI          152  GGCACC               G^G[CT][GA]CC
BanII         668  GAGCCC               G[GA]GC[CT]^C
BcnI          495  CCCGG                CC^[GC]GG
BsaAI         883  TACGTG               [CT]AC^GT[GA]
BsaBI         513  GATAAGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI        1222  CTCCTC               GAGGAG(10/8)
BsoBI         263  CTCGAG               C^[CT]CG[GA]G
BspHI        1265  TCATGA               T^CATGA
BspMI          21  GCAGGT               ACCTGC(4/8)
BsrBI          98  CCGCTC               CCGCTC(-3/-3)
BstBI         695  TTCGAA               TT^CGAA
Cac8I         615  GCAGGC               GC[ACGT]^[ACGT]GC
CfoI         1402  GCGC                 GCG^C
DraI          685  TTTAAA               TTT^AAA
EcoRI        1033  GAATTC               G^AATTC
EcoT14I      1394  CCTTGG               C^C[AT][AT]GG
EcoT22I       471  ATGCAT               ATGCA^T
HapII         496  CCGG                 C^CGG
HhaI         1402  GCGC                 GCG^C
HinP1I       1402  GCGC                 G^CGC
HpaII         496  CCGG                 C^CGG
MaeIII       1387  GTTAC                ^GT[ACGT]AC
MamI          513  GATAAGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I        332  CAGCTG               C[AC]G^C[GT]G
MspI          496  CCGG                 C^CGG
NciI          495  CCCGG                CC^[GC]GG
NsiI          471  ATGCAT               ATGCA^T
NspV          695  TTCGAA               TT^CGAA
PacI          175  TTAATTAA             TTAAT^TAA
PaeR7I        263  CTCGAG               C^TCGAG
PflFI         429  GACGAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         930  CCAAACTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          283  GAGTC                GAGTC(4/5)
PshAI         206  GACTGAAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII         332  CAGCTG               CAG^CTG
RcaI         1265  TCATGA               T^CATGA
SapI          100  GCTCTTC              GCTCTTC(1/4)
SexAI           7  ACCTGGT              A^CC[AT]GGT
SfaNI         315  GATGC                GCATC(5/9)
SfuI          695  TTCGAA               TT^CGAA
SspI         1141  AATATT               AAT^ATT
StyI         1394  CCTTGG               C^C[AT][AT]GG
Tth111I       429  GACGAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        930  CCAAACTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI         1289  CCACACCTGTGTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI          263  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

AfaI          570  GTAC                 GT^AC
              588  GTAC                
AflIII       1184  ACATGT               A^C[GA][CT]GT
             1213  ACATGT              
BsaJI         239  CCCTGG               C^C[ACGT][ACGT]GG
             1394  CCTTGG              
BsmFI         213  GTCCC                GGGAC(10/14)
             1452  GTCCC               
BspLU11I     1184  ACATGT               A^CATGT
             1213  ACATGT              
Cfr13I         95  GGGCC                G^G[ACGT]CC
              618  GGCCC               
DdeI          548  CTAAG                C^T[ACGT]AG
              845  CTGAG               
EcoNI         609  CCTGCTGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              630  CCTGGATGAGG         
MaeII         596  ACGT                 A^CGT
              884  ACGT                
PstI           27  CTGCAG               CTGCA^G
              613  CTGCAG              
RsaI          570  GTAC                 GT^AC
              588  GTAC                
Sau96I         95  GGGCC                G^G[ACGT]CC
              618  GGCCC               
SfcI           27  CTGCAG               C^T[GA][CT]AG
              613  CTGCAG              
SmlI          200  CTCAAG               C^T[CT][GA]AG
              263  CTCGAG              
TfiI          841  GATTC                G^A[AT]TC
             1087  GAATC               


#########################################
# 3 OR MORE CUT ENZYMES (   46 enzymes) #
#########################################

AcsI        AluI        AlwI        ApoI        BbsI        
BbvI        BfaI        BmyI        BpmI        BpuAI       
BsiYI       BslI        Bsp1286I    BsrI        BssKI       
BstF5I      BstNI       DpnII       EarI        EcoRII      
Fnu4HI      FokI        HaeIII      HinfI       ItaI        
Ksp632I     MaeI        MboI        MboII       MnlI        
MseI        MslI        MvaI        MwoI        NdeII       
NlaIII      NlaIV       NspI        Sau3AI      ScrFI       
TaqI        Tru9I       TseI        Tsp509I     TspRI       
TthHB8I     
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