Restriction map for pF1KE0041
 (Linear) (Six-base) MAPSORT of: KIEE0041.seq  Check: 8299  from: 1  to: 1560



KIEE0041.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:09  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     229     997    1560 
   Size:        229     768     563

  Fragments arranged by size: 

                 768     563     229
 
AflIII A'CryG_T

Cuts at:      0    1184    1213    1560 
   Size:       1184      29     347

  Fragments arranged by size: 

                1184     347      29
 
AleI CACnn'nnGTG

Cuts at:      0    1294    1372    1560 
   Size:       1294      78     188

  Fragments arranged by size: 

                1294     188      78
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      47      79    1560 
   Size:         47      32    1481

  Fragments arranged by size: 

                1481      47      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     135    1560 
   Size:        135    1425
 
ApoI r'AATT_y

Cuts at:      0      48     398     742    1033    1336    1560 
   Size:         48     350     344     291     303     224

  Fragments arranged by size: 

                 350     344     303     291     224      48
 
AvaI C'yCGr_G

Cuts at:      0     263    1560 
   Size:        263    1297
 
BanI G'GyrC_C

Cuts at:      0     152    1560 
   Size:        152    1408
 
BanII G_rGCy'C

Cuts at:      0     672    1560 
   Size:        672     888
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     705    1187    1452    1560 
   Size:        705     482     265     108

  Fragments arranged by size: 

                 705     482     265     108
 
BfrBI ATG'CAT

Cuts at:      0     473    1560 
   Size:        473    1087
 
Bme1580I G_kGCm'C

Cuts at:      0     155     496    1015    1560 
   Size:        155     341     519     545

  Fragments arranged by size: 

                 545     519     341     155
 
BmgBI CAC'GTC

Cuts at:      0     597    1560 
   Size:        597     963
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     273     517     924    1560 
   Size:        273     244     407     636

  Fragments arranged by size: 

                 636     407     273     244
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     185    1560 
   Size:        185    1375
 
BsaAI yAC'GTr

Cuts at:      0     885    1560 
   Size:        885     675
 
BsaBI GATnn'nnATC

Cuts at:      0     517    1560 
   Size:        517    1043
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      47      77    1560 
   Size:         47      30    1483

  Fragments arranged by size: 

                1483      47      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1213    1560 
   Size:       1213     347
 
BseYI C'CCAG_C

Cuts at:      0     574     581    1249    1560 
   Size:        574       7     668     311

  Fragments arranged by size: 

                 668     574     311       7
 
Bsp1286I G_dGCh'C

Cuts at:      0     155     496     672    1015    1560 
   Size:        155     341     176     343     545

  Fragments arranged by size: 

                 545     343     341     176     155
 
BspHI T'CATG_A

Cuts at:      0    1265    1560 
   Size:       1265     295
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      12    1560 
   Size:         12    1548
 
BsrBI CCG'CTC

Cuts at:      0     100    1560 
   Size:        100    1460
 
BstBI TT'CG_AA

Cuts at:      0     696    1560 
   Size:        696     864
 
DraI TTT'AAA

Cuts at:      0     687    1560 
   Size:        687     873
 
EarI CTCTTCn'nnn_

Cuts at:      0     107     275     750    1560 
   Size:        107     168     475     810

  Fragments arranged by size: 

                 810     475     168     107
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     229     273     517     924     997    1560 
   Size:        229      44     244     407      73     563

  Fragments arranged by size: 

                 563     407     244     229      73      44
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     613     634    1560 
   Size:        613      21     926

  Fragments arranged by size: 

                 926     613      21
 
EcoRI G'AATT_C

Cuts at:      0    1033    1560 
   Size:       1033     527
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     268     300     537     569    1422    1454    1560 
   Size:        268      32     237      32     853      32     106

  Fragments arranged by size: 

                 853     268     237     106      32      32      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1062    1280    1560 
   Size:       1062     218     280

  Fragments arranged by size: 

                1062     280     218
 
MslI CAynn'nnrTG

Cuts at:      0     423    1201    1294    1372    1560 
   Size:        423     778      93      78     188

  Fragments arranged by size: 

                 778     423     188      93      78
 
MspA1I CmG'CkG

Cuts at:      0     334    1560 
   Size:        334    1226
 
NsiI A_TGCA'T

Cuts at:      0     475    1560 
   Size:        475    1085
 
NspI r_CATG'y

Cuts at:      0     477    1188    1217    1560 
   Size:        477     711      29     343

  Fragments arranged by size: 

                 711     477     343      29
 
PacI TTA_AT'TAA

Cuts at:      0     179    1560 
   Size:        179    1381
 
PciI A'CATG_T

Cuts at:      0    1184    1213    1560 
   Size:       1184      29     347

  Fragments arranged by size: 

                1184     347      29
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     936    1560 
   Size:        936     624
 
PfoI T'CCnGG_A

Cuts at:      0     629    1560 
   Size:        629     931
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      47      79    1560 
   Size:         47      32    1481

  Fragments arranged by size: 

                1481      47      32
 
PshAI GACnn'nnGTC

Cuts at:      0     210    1560 
   Size:        210    1350
 
PsiI TTA'TAA

Cuts at:      0    1064    1560 
   Size:       1064     496
 
PstI C_TGCA'G

Cuts at:      0      31     617    1560 
   Size:         31     586     943

  Fragments arranged by size: 

                 943     586      31
 
PvuII CAG'CTG

Cuts at:      0     334    1560 
   Size:        334    1226
 
SapI GCTCTTCn'nnn_

Cuts at:      0     107    1560 
   Size:        107    1453
 
SexAI A'CCwGG_T

Cuts at:      0       7    1560 
   Size:          7    1553
 
SfcI C'TryA_G

Cuts at:      0      27     613    1560 
   Size:         27     586     947

  Fragments arranged by size: 

                 947     586      27
 
SmlI C'TyrA_G

Cuts at:      0     200     263    1560 
   Size:        200      63    1297

  Fragments arranged by size: 

                1297     200      63
 
SspI AAT'ATT

Cuts at:      0    1143    1560 
   Size:       1143     417
 
StyI C'CwwG_G

Cuts at:      0    1394    1560 
   Size:       1394     166
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     595    1560 
   Size:        595     965
 
TatI w'GTAC_w

Cuts at:      0     587    1560 
   Size:        587     973
 
Tth111I GACn'n_nGTC

Cuts at:      0     432    1560 
   Size:        432    1128
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1296    1560 
   Size:       1296     264
 
XhoI C'TCGA_G

Cuts at:      0     263    1560 
   Size:        263    1297
 

 Enzymes that do cut:

     AcuI   AflIII     AleI     AloI    AlwNI     ApoI     AvaI     BanI
    BanII     BbsI    BfrBI Bme1580I    BmgBI     BpmI    BpuEI    BsaAI
    BsaBI    BsaXI    BseRI    BseYI Bsp1286I    BspHI    BspMI    BsrBI
    BstBI     DraI     EarI  Eco57MI    EcoNI    EcoRI    Hin4I     MmeI
     MslI   MspA1I     NsiI     NspI     PacI     PciI    PflMI     PfoI
     PpiI    PshAI     PsiI     PstI    PvuII     SapI    SexAI     SfcI
     SmlI     SspI     StyI    TaqII     TatI  Tth111I     XcmI     XhoI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AlfI     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI     BbeI    BbvCI     BcgI    BciVI     BclI     BglI
    BglII     BlpI     BmrI     BmtI     BplI   Bpu10I     BsaI    BsaHI
    BsaWI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI   BstEII    BstXI
    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI
   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI EcoO109I    EcoRV
     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MscI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     PasI     PmeI     PmlI
    PpuMI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     StuI     SwaI     TstI
     XbaI     XmaI     XmnI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com