Restriction map for pF1KB6753
 (Circular) (Six-base) MAPSORT of: pF1KB6753.seq  Check: 4905  from: 1  to: 3428



pF1KB6753  3428 bp

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:48  ..

AccI GT'mk_AC

Cuts at:    466     466 
   Size:       3428
 
Acc65I G'GTAC_C

Cuts at:    444     444 
   Size:       3428
 
AclI AA'CG_TT

Cuts at:   3268    3268 
   Size:       3428
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1253    1685    2432    2700    1253 
   Size:        432     747     268    1981

  Fragments arranged by size: 

                1981     747     432     268
 
AflIII A'CryG_T

Cuts at:    780    1884    2861     780 
   Size:       1104     977    1347

  Fragments arranged by size: 

                1347    1104     977
 
AgeI A'CCGG_T

Cuts at:   1816    1816 
   Size:       3428
 
AlwNI CAG_nnn'CTG

Cuts at:    136     733    2300     136 
   Size:        597    1567    1264

  Fragments arranged by size: 

                1567    1264     597
 
ApaLI G'TGCA_C

Cuts at:   2198    2198 
   Size:       3428
 
ApoI r'AATT_y

Cuts at:    343     432    2781    3037     343 
   Size:         89    2349     256     734

  Fragments arranged by size: 

                2349     734     256      89
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3428
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3428
 
AvaI C'yCGr_G

Cuts at:    448     448 
   Size:       3428
 
BamHI G'GATC_C

Cuts at:    453     453 
   Size:       3428
 
BanI G'GyrC_C

Cuts at:    444     858    1106    1141     444 
   Size:        414     248      35    2731

  Fragments arranged by size: 

                2731     414     248      35
 
BanII G_rGCy'C

Cuts at:    442    1472     442 
   Size:       1030    2398
 
BbeI G_GCGC'C

Cuts at:    862    1110     862 
   Size:        248    3180
 
BbsI GAAGACnn'nnnn_

Cuts at:    159     159 
   Size:       3428
 
BciVI GTATCCnnnnn_n'

Cuts at:   1326    2087    3428    1326 
   Size:        761    1341    1326

  Fragments arranged by size: 

                1341    1326     761
 
BclI T'GATC_A

Cuts at:    948     948 
   Size:       3428
 
BglII A'GATC_T

Cuts at:    943    2980     943 
   Size:       2037    1391
 
BlpI GC'TnA_GC

Cuts at:    540     540 
   Size:       3428
 
Bme1580I G_kGCm'C

Cuts at:   1053    1146    2202    1053 
   Size:         93    1056    2279

  Fragments arranged by size: 

                2279    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    640     721    1056    3200     640 
   Size:         81     335    2144     868

  Fragments arranged by size: 

                2144     868     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1795    1795 
   Size:       3428
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    105     105 
   Size:       3428
 
Bpu10I CC'TnA_GC

Cuts at:    226     226 
   Size:       3428
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    379     604    1483    1975    2273     379 
   Size:        225     879     492     298    1534

  Fragments arranged by size: 

                1534     879     492     298     225
 
BsaI GGTCTCn'nnnn_

Cuts at:   3132    3132 
   Size:       3428
 
BsaAI yAC'GTr

Cuts at:    781     781 
   Size:       3428
 
BsaBI GATnn'nnATC

Cuts at:      6     942    2979       6 
   Size:        936    2037     455

  Fragments arranged by size: 

                2037     936     455
 
BsaHI Gr'CG_yC

Cuts at:    859    1107    1809    3305     859 
   Size:        248     702    1496     982

  Fragments arranged by size: 

                1496     982     702     248
 
BsaWI w'CCGG_w

Cuts at:    617    1138    1816    2090    2237    2648     617 
   Size:        521     678     274     147     411    1397

  Fragments arranged by size: 

                1397     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    193     223     193 
   Size:         30    3398
 
BseYI C'CCAG_C

Cuts at:    853    1611    2188    2684     853 
   Size:        758     577     496    1597

  Fragments arranged by size: 

                1597     758     577     496
 
BsiEI CG_ry'CG

Cuts at:    104     970    1016    2224     104 
   Size:        866      46    1208    1308

  Fragments arranged by size: 

                1308    1208     866      46
 
BsiHKAI G_wGCw'C

Cuts at:    442    1220    1410    2202     442 
   Size:        778     190     792    1668

  Fragments arranged by size: 

                1668     792     778     190
 
Bsp1286I G_dGCh'C

Cuts at:    442    1053    1146    1220    1410    1472    2202     442 
   Size:        611      93      74     190      62     730    1668

  Fragments arranged by size: 

                1668     730     611     190      93      74      62
 
BspEI T'CCGG_A

Cuts at:   2648    2648 
   Size:       3428
 
BspHI T'CATG_A

Cuts at:    233    2604     233 
   Size:       2371    1057
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    478     692     994    1375     478 
   Size:        214     302     381    2531

  Fragments arranged by size: 

                2531     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50     141    1720    1774    3258    3423      50 
   Size:         91    1579      54    1484     165      55

  Fragments arranged by size: 

                1579    1484     165      91      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1340    1340 
   Size:       3428
 
BsrFI r'CCGG_y

Cuts at:   1426    1607    1816    1426 
   Size:        181     209    3038

  Fragments arranged by size: 

                3038     209     181
 
BssSI C'ACGA_G

Cuts at:   1699    2057    1699 
   Size:        358    3070
 
BstAPI GCAn_nnn'nTGC

Cuts at:    805     805 
   Size:       3428
 
BstBI TT'CG_AA

Cuts at:   1789    1789 
   Size:       3428
 
BstYI r'GATC_y

Cuts at:      1     453     920     943    1278    1524    2536    2622    2634 
   Size:        452     467      23     335     246    1012      86      12

Cuts at:   2634    2980       1 
   Size:        346     449

  Fragments arranged by size: 

                1012     467     452     449     346     335     246      86
                  23      12
 
BtgI C'CryG_G

Cuts at:    107     107 
   Size:       3428
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1378    1559    1722    1378 
   Size:        181     163    3084

  Fragments arranged by size: 

                3084     181     163
 
BtsI GCAGTG_nn'

Cuts at:    671     671 
   Size:       3428
 
DraI TTT'AAA

Cuts at:    427     427 
   Size:       3428
 
DrdI GACnn_nn'nnGTC

Cuts at:    280    1134    1992     280 
   Size:        854     858    1716

  Fragments arranged by size: 

                1716     858     854
 
EaeI y'GGCC_r

Cuts at:   1013    1187    1578    1605    2950    1013 
   Size:        174     391      27    1345    1491

  Fragments arranged by size: 

                1491    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   1013    1013 
   Size:       3428
 
EarI CTCTTCn'nnn_

Cuts at:   1451    1661    1451 
   Size:        210    3218
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1636    1946    2092    3238    1636 
   Size:        310     146    1146    1826

  Fragments arranged by size: 

                1826    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    440     440 
   Size:       3428
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    105    1253    1685    2432    2700     105 
   Size:       1148     432     747     268     833

  Fragments arranged by size: 

                1148     833     747     432     268
 
EcoO109I rG'GnC_Cy

Cuts at:    273     567     273 
   Size:        294    3134
 
EcoRI G'AATT_C

Cuts at:    432     432 
   Size:       3428
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1447    1447 
   Size:       3428
 
FspI TGC'GCA

Cuts at:   1209    1209 
   Size:       3428
 
HaeII r_GCGC'y

Cuts at:    862    1110    2132    3111     862 
   Size:        248    1022     979    1179

  Fragments arranged by size: 

                1179    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1270    1300    1302    1332    1270 
   Size:         30       2      30    3366

  Fragments arranged by size: 

                3366      30      30       2
 
HincII GTy'rAC

Cuts at:    467     467 
   Size:       3428
 
HindIII A'AGCT_T

Cuts at:    228     228 
   Size:       3428
 
KasI G'GCGC_C

Cuts at:    858    1106     858 
   Size:        248    3180
 
KpnI G_GTAC'C

Cuts at:    448     448 
   Size:       3428
 
MluI A'CGCG_T

Cuts at:   2861    2861 
   Size:       3428
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2099    2283    2099 
   Size:        184    3244
 
MscI TGG'CCA

Cuts at:   1189    1189 
   Size:       3428
 
MslI CAynn'nnrTG

Cuts at:   1417    1544    1417 
   Size:        127    3301
 
MspA1I CmG'CkG

Cuts at:    539     853    1213    2226    2471     539 
   Size:        314     360    1013     245    1496

  Fragments arranged by size: 

                1496    1013     360     314     245
 
NaeI GCC'GGC

Cuts at:   1609    1609 
   Size:       3428
 
NarI GG'CG_CC

Cuts at:    859    1107     859 
   Size:        248    3180
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       3428
 
NgoMIV G'CCGG_C

Cuts at:   1607    1607 
   Size:       3428
 
NspI r_CATG'y

Cuts at:    481     981    1888     481 
   Size:        500     907    2021

  Fragments arranged by size: 

                2021     907     500
 
PciI A'CATG_T

Cuts at:   1884    1884 
   Size:       3428
 
PmeI GTTT'AAAC

Cuts at:    427     427 
   Size:       3428
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2567    2599    2567 
   Size:         32    3396
 
PpuMI rG'GwC_Cy

Cuts at:    273     273 
   Size:       3428
 
PsiI TTA'TAA

Cuts at:    197     197 
   Size:       3428
 
PstI C_TGCA'G

Cuts at:    475    1160     475 
   Size:        685    2743
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3428
 
PvuII CAG'CTG

Cuts at:    853    1213     853 
   Size:        360    3068
 
RsrII CG'GwC_CG

Cuts at:    419    1623     419 
   Size:       1204    2224
 
SacI G_AGCT'C

Cuts at:    442     442 
   Size:       3428
 
SalI G'TCGA_C

Cuts at:    465     465 
   Size:       3428
 
SapI GCTCTTCn'nnn_

Cuts at:   1451    1661    1451 
   Size:        210    3218
 
SbfI CC_TGCA'GG

Cuts at:    475     475 
   Size:       3428
 
ScaI AGT'ACT

Cuts at:   2945    2945 
   Size:       3428
 
SfcI C'TryA_G

Cuts at:     33     128     471    1156    2149    2340      33 
   Size:         95     343     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     343     191      95
 
SfoI GGC'GCC

Cuts at:    860    1108     860 
   Size:        248    3180
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3428
 
SmaI CCC'GGG

Cuts at:    450     450 
   Size:       3428
 
SmlI C'TyrA_G

Cuts at:    394     583    1498    1990    2252     394 
   Size:        189     915     492     262    1570

  Fragments arranged by size: 

                1570     915     492     262     189
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3428
 
SphI G_CATG'C

Cuts at:    481     981     481 
   Size:        500    2928
 
StyI C'CwwG_G

Cuts at:    107     562     916     107 
   Size:        455     354    2619

  Fragments arranged by size: 

                2619     455     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1011    1144    1603    1812    1011 
   Size:        133     459     209    2627

  Fragments arranged by size: 

                2627     459     209     133
 
TatI w'GTAC_w

Cuts at:   2943    2943 
   Size:       3428
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2945    2977    2945 
   Size:         32    3396
 
Tth111I GACn'n_nGTC

Cuts at:   1225    1225 
   Size:       3428
 
XbaI T'CTAG_A

Cuts at:    459     459 
   Size:       3428
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    178     274     923     178 
   Size:         96     649    2683

  Fragments arranged by size: 

                2683     649      96
 
XmaI C'CCGG_G

Cuts at:    448     448 
   Size:       3428
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI    ApaLI
     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbeI
     BbsI    BciVI     BclI    BglII     BlpI Bme1580I     BmrI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseYI    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI    BstYI     BtgI
    BtgZI     BtsI     DraI     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I
   HincII  HindIII     KasI     KpnI     MluI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI     PciI     PmeI
     PpiI    PpuMI     PsiI     PstI     PvuI    PvuII    RsrII     SacI
     SalI     SapI     SbfI     ScaI     SfcI     SfoI     SgfI     SmaI
     SmlI     SpeI     SphI     StyI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI
     ApaI     AscI    AvrII     BaeI    BbvCI     BcgI    BfrBI     BglI
    BmgBI     BmtI    BseRI     BsgI    BsiWI     BsmI    BsmBI    BsrGI
   BssHII   BstEII    BstXI  BstZ17I   Bsu36I     ClaI    CspCI   DraIII
    EcoNI    EcoRV     FseI    FspAI     HpaI     MfeI     NdeI     NheI
     NotI     NruI     NsiI     PacI     PasI    PflMI     PfoI     PmlI
    PshAI   PspOMI    PspXI     PsrI    SacII    SanDI    SexAI     SfiI
    SgrAI    SnaBI     SrfI     SspI     StuI     SwaI     XhoI     XmnI
     ZraI
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