Restriction map for pF1KB8903
##################################
# NO CUT ENZYMES (  146 enzymes) #
##################################

AccI        AccIII      AclI        AflII       AflIII      
AgeI        AhdI        AluI        Alw44I      Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
AspEI       AspHI       AspI        AviII       AvrII       
BalI        BamHI       BanII       BbrPI       BbsI        
BbvCI       BclI        BfaI        BfrI        BglI        
BlnI        BlpI        BmyI        Bpu1102I    BpuAI       
BsaAI       BsaI        BseAI       BsgI        BsiHKAI     
BsiWI       BsmBI       BsmFI       BsmI        Bsp1286I    
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrDI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstXI       BstZ17I     CelII       ClaI        DraI        
DraII       DraIII      DrdI        DsaI        Eam1105I    
Eco47III    EcoNI       EcoO109I    EcoO65I     EcoRV       
EcoT14I     EcoT22I     FbaI        FseI        FspI        
HgaI        HincII      HindII      HindIII     HpaI        
HphI        KspI        MaeI        MaeIII      MfeI        
MluI        MluNI       MroI        MscI        MseI        
MslI        MspA1I      MunI        NcoI        NdeI        
NheI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PvuI        PvuII       RcaI        SacI        SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfuI        SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        StyI        SwaI        Tru9I       Tsp45I      
Tth111I     XbaI        XcmI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AatII          55  GACGTC               GACGT^C
Acc65I        449  GGTACC               G^GTACC
AfaI          450  GTAC                 GT^AC
AlwNI         454  CAGACCCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          272  CCTTAGG              CC^T[ACGT]AGG
Asp700I       383  GAATGAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I       449  GGTACC               G^GTACC
AvaI          618  CTCGGG               C^[CT]CG[GA]G
BbeI          503  GGCGCC               GGCGC^C
BbvI          378  GCAGC                GCAGC(8/12)
BcgI          512  GCATCGAGATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI         356  GTATCC               GTATCC(6/5)
BglII         437  AGATCT               A^GATCT
BpmI          466  CTGGAG               CTGGAG(16/14)
BsaBI         552  GATCAAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI         688  GAGGAG               GAGGAG(10/8)
BsmAI         679  GAGAC                GTCTC(1/5)
BsoBI         618  CTCGGG               C^[CT]CG[GA]G
BsrFI         506  GCCGGC               [GA]^CCGG[CT]
Bsu36I        272  CCTTAGG              CC^T[ACGT]AGG
Cac8I         506  GCCGGC               GC[ACGT]^[ACGT]GC
Cfr10I        506  GCCGGC               [GA]^CCGG[CT]
CpoI          416  CGGACCG              CG^G[AT]CCG
Eco81I        272  CCTTAGG              CC^T[ACGT]AGG
FauI          672  GCGGG                CCCGC(4/6)
HaeII         503  GGCGCC               [GA]GCGC^[CT]
KasI          503  GGCGCC               G^GCGCC
KpnI          449  GGTACC               GGTAC^C
MamI          552  GATCAAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI          506  GCCGGC               GCC^GGC
NarI          503  GGCGCC               GG^CGCC
NgoMIV        506  GCCGGC               G^CCGGC
NlaIII        246  CATG                 CATG^
NotI          428  GCGGCCGC             GC^GGCCGC
NspI          245  ACATGC               [GA]CATG^[CT]
PflMI         453  CCAGACCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          153  GACTC                GAGTC(4/5)
PstI          258  CTGCAG               CTGCA^G
RsaI          450  GTAC                 GT^AC
RsrII         416  CGGACCG              CG^G[AT]CCG
SfcI          258  CTGCAG               C^T[GA][CT]AG
SfoI          503  GGCGCC               GGC^GCC
SgrAI         505  CGCCGGCG             C[GA]^CCGG[CT]G
TfiI          595  GAATC                G^A[AT]TC
TseI          378  GCAGC                G^C[AT]GC
Van91I        453  CCAGACCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI          383  GAATGAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AcyI           55  GACGTC               G[GA]^CG[CT]C
              503  GGCGCC              
AvaII         342  GGACC                G^G[AT]CC
              417  GGACC               
BanI          449  GGTACC               G^G[CT][GA]CC
              503  GGCGCC              
BcnI           44  CCGGG                CC^[GC]GG
              198  CCGGG               
BsaHI          55  GACGTC               G[GA]^CG[CT]C
              503  GGCGCC              
BsaWI         140  ACCGGA               [AT]^CCGG[AT]
              419  ACCGGA              
BsiEI         125  CGGCCG               CG[GA][CT]^CG
              429  CGGCCG              
BsrBI         122  GAGCGG               CCGCTC(-3/-3)
              413  GAGCGG              
EaeI          125  CGGCCG               [CT]^GGCC[GA]
              429  CGGCCG              
EagI          125  CGGCCG               C^GGCCG
              429  CGGCCG              
EarI          676  GAAGAG               CTCTTC(1/4)
              685  GAAGAG              
EclXI         125  CGGCCG               C^GGCCG
              429  CGGCCG              
Eco52I        125  CGGCCG               C^GGCCG
              429  CGGCCG              
EcoRI           3  GAATTC               G^AATTC
              387  GAATTC              
Hin1I          55  GACGTC               G[GA]^CG[CT]C
              503  GGCGCC              
HinfI         153  GACTC                G^A[ACGT]TC
              595  GAATC               
Ksp632I       676  GAAGAG               CTCTTC(1/4)
              685  GAAGAG              
MaeII          56  ACGT                 A^CGT
               68  ACGT                
MboII         676  GAAGA                GAAGA(8/7)
              685  GAAGA               
MwoI          124  GCGGCCGTTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              501  GCGGCGCCGGC         
NciI           44  CCGGG                CC^[GC]GG
              198  CCGGG               
SfaNI         375  GATGC                GCATC(5/9)
              512  GCATC               
TspRI          98  CAGTG                CA[GC]TG[ACGT][ACGT]^
              396  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   42 enzymes) #
#########################################

AccII       AciI        AcsI        AlwI        ApoI        
BsaJI       BsiYI       BslI        BsrI        BssKI       
BstF5I      BstNI       BstUI       BstYI       CfoI        
Cfr13I      DdeI        DpnII       EcoRII      Fnu4HI      
FokI        HaeIII      HapII       HhaI        HinP1I      
HpaII       ItaI        MboI        MflI        MnlI        
MspI        MvaI        MvnI        NdeII       NlaIV       
Sau3AI      Sau96I      ScrFI       TaqI        Tsp509I     
TthHB8I     XhoII       
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