Restriction map for pF1KB8903
 (Linear) (Six-base) MAPSORT of: KIBB8903.seq  Check: 9406  from: 1  to: 705



KIBB8903.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:29  ..

AatII G_ACGT'C

Cuts at:      0      59     705 
   Size:         59     646
 
Acc65I G'GTAC_C

Cuts at:      0     449     705 
   Size:        449     256
 
AlwNI CAG_nnn'CTG

Cuts at:      0     459     705 
   Size:        459     246
 
ApoI r'AATT_y

Cuts at:      0       3     387     475     705 
   Size:          3     384      88     230

  Fragments arranged by size: 

                 384     230      88       3
 
AvaI C'yCGr_G

Cuts at:      0     618     705 
   Size:        618      87
 
BanI G'GyrC_C

Cuts at:      0     449     503     705 
   Size:        449      54     202

  Fragments arranged by size: 

                 449     202      54
 
BbeI G_GCGC'C

Cuts at:      0     507     705 
   Size:        507     198
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     501     535     705 
   Size:        501      34     170

  Fragments arranged by size: 

                 501     170      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     367     705 
   Size:        367     338
 
BglII A'GATC_T

Cuts at:      0     437     705 
   Size:        437     268
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     487     705 
   Size:        487     218
 
Bpu10I CC'TnA_GC

Cuts at:      0     302     705 
   Size:        302     403
 
BsaBI GATnn'nnATC

Cuts at:      0     556     705 
   Size:        556     149
 
BsaHI Gr'CG_yC

Cuts at:      0      56     504     705 
   Size:         56     448     201

  Fragments arranged by size: 

                 448     201      56
 
BsaWI w'CCGG_w

Cuts at:      0     140     419     705 
   Size:        140     279     286

  Fragments arranged by size: 

                 286     279     140
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     113     143     705 
   Size:        113      30     562

  Fragments arranged by size: 

                 562     113      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     703     705 
   Size:        703       2
 
BsiEI CG_ry'CG

Cuts at:      0     128     432     705 
   Size:        128     304     273

  Fragments arranged by size: 

                 304     273     128
 
BsrBI CCG'CTC

Cuts at:      0     124     415     705 
   Size:        124     291     290

  Fragments arranged by size: 

                 291     290     124
 
BsrFI r'CCGG_y

Cuts at:      0     506     705 
   Size:        506     199
 
BstYI r'GATC_y

Cuts at:      0     143     217     437     705 
   Size:        143      74     220     268

  Fragments arranged by size: 

                 268     220     143      74
 
Bsu36I CC'TnA_GG

Cuts at:      0     273     705 
   Size:        273     432
 
EaeI y'GGCC_r

Cuts at:      0     125     429     705 
   Size:        125     304     276

  Fragments arranged by size: 

                 304     276     125
 
EagI C'GGCC_G

Cuts at:      0     125     429     705 
   Size:        125     304     276

  Fragments arranged by size: 

                 304     276     125
 
EarI CTCTTCn'nnn_

Cuts at:      0     671     680     705 
   Size:        671       9      25

  Fragments arranged by size: 

                 671      25       9
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     644     671     705 
   Size:        644      27      34

  Fragments arranged by size: 

                 644      34      27
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     487     705 
   Size:        487     218
 
EcoRI G'AATT_C

Cuts at:      0       3     387     705 
   Size:          3     384     318

  Fragments arranged by size: 

                 384     318       3
 
HaeII r_GCGC'y

Cuts at:      0     507     705 
   Size:        507     198
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     202     234     235     267     705 
   Size:        202      32       1      32     438

  Fragments arranged by size: 

                 438     202      32      32       1
 
KasI G'GCGC_C

Cuts at:      0     503     705 
   Size:        503     202
 
KpnI G_GTAC'C

Cuts at:      0     453     705 
   Size:        453     252
 
NaeI GCC'GGC

Cuts at:      0     508     705 
   Size:        508     197
 
NarI GG'CG_CC

Cuts at:      0     504     705 
   Size:        504     201
 
NgoMIV G'CCGG_C

Cuts at:      0     506     705 
   Size:        506     199
 
NotI GC'GGCC_GC

Cuts at:      0     429     705 
   Size:        429     276
 
NspI r_CATG'y

Cuts at:      0     249     705 
   Size:        249     456
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     459     705 
   Size:        459     246
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     130     162     705 
   Size:        130      32     543

  Fragments arranged by size: 

                 543     130      32
 
PstI C_TGCA'G

Cuts at:      0     262     705 
   Size:        262     443
 
RsrII CG'GwC_CG

Cuts at:      0     417     705 
   Size:        417     288
 
SfcI C'TryA_G

Cuts at:      0     258     705 
   Size:        258     447
 
SfoI GGC'GCC

Cuts at:      0     505     705 
   Size:        505     200
 
SgrAI Cr'CCGG_yG

Cuts at:      0     506     705 
   Size:        506     199
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     556     705 
   Size:        556     149
 
XmnI GAAnn'nnTTC

Cuts at:      0     387     705 
   Size:        387     318
 
ZraI GAC'GTC

Cuts at:      0      57     705 
   Size:         57     648
 

 Enzymes that do cut:

    AatII   Acc65I    AlwNI     ApoI     AvaI     BanI     BbeI     BcgI
    BciVI    BglII     BpmI   Bpu10I    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BsiEI    BsrBI    BsrFI    BstYI   Bsu36I     EaeI     EagI
     EarI     EciI  Eco57MI    EcoRI    HaeII    Hin4I     KasI     KpnI
     NaeI     NarI   NgoMIV     NotI     NspI    PflMI     PsrI     PstI
    RsrII     SfcI     SfoI    SgrAI    TaqII     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AccI     AclI     AcuI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI    BanII     BbsI    BbvCI     BclI
    BfrBI     BglI     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI
    BpuEI     BsaI    BsaAI    BseYI     BsgI  BsiHKAI    BsiWI     BsmI
    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrDI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI  EcoICRI    EcoNI
 EcoO109I    EcoRV     FalI     FseI     FspI    FspAI   HincII  HindIII
     HpaI     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NcoI
     NdeI     NheI     NruI     NsiI     PacI     PasI     PciI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PvuI    PvuII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SgfI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     StyI     SwaI     TatI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI
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