Restriction map for pF1KE0539
##################################
# NO CUT ENZYMES (  103 enzymes) #
##################################

AclI        AcsI        AflIII      AgeI        AhdI        
Alw44I      AlwNI       Aor51HI     ApaLI       ApoI        
AscI        AseI        AsnI        AspEI       AvrII       
BbeI        BbrPI       BbsI        BbvCI       BfaI        
BglI        BglII       BlnI        BpuAI       BsaI        
BsiEI       BsiWI       BsmBI       Bsp1407I    BspDI       
BspHI       BspLU11I    BsrGI       BssHII      Bst1107I    
BstAPI      BstBI       BstZ17I     ClaI        CpoI        
DraI        DrdI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoRI       EcoRV       EcoT22I     
FseI        HaeII       HgaI        HindIII     HpaI        
KasI        KspI        MaeI        MfeI        MluI        
MunI        NaeI        NarI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PinAI       PmaCI       PmeI        
PmlI        PshBI       Psp1406I    PstI        PvuI        
RcaI        RsrII       SacI        SacII       SbfI        
SexAI       SfcI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        SwaI        TfiI        
XbaI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   69 enzymes) #
###################################

AatII         652  GACGTC               GACGT^C
Acc65I        612  GGTACC               G^GTACC
AccI          178  GTCGAC               GT^[AC][GT]AC
AccIII        296  TCCGGA               T^CCGGA
AcyI          652  GACGTC               G[GA]^CG[CT]C
AflII        1093  CTTAAG               C^TTAAG
AocI           97  CCTAAGG              CC^T[ACGT]AGG
ApaI          587  GGGCCC               GGGCC^C
Asp718I       612  GGTACC               G^GTACC
AspI          985  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          287  CTCGAG               C^[CT]CG[GA]G
AviII         743  TGCGCA               TGC^GCA
BamHI         190  GGATCC               G^GATCC
BanI          612  GGTACC               G^G[CT][GA]CC
BcgI          771  GCATCCTGCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI         142  GGATAC               GTATCC(6/5)
BclI          893  TGATCA               T^GATCA
BcnI          187  CCGGG                CC^[GC]GG
BfrI         1093  CTTAAG               C^TTAAG
BlpI          319  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      319  GCTGAGC              GC^T[ACGT]AGC
BsaAI         274  TACGTG               [CT]AC^GT[GA]
BsaBI         540  GATGGAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI         652  GACGTC               G[GA]^CG[CT]C
BsaWI         296  TCCGGA               [AT]^CCGG[AT]
BseAI         296  TCCGGA               T^CCGGA
BseRI        1147  GAGGAG               GAGGAG(10/8)
BsgI         1057  GTGCAG               GTGCAG(16/14)
BsoBI         287  CTCGAG               C^[CT]CG[GA]G
BspEI         296  TCCGGA               T^CCGGA
BspMI         609  GCAGGT               ACCTGC(4/8)
BsrBI          20  CCGCTC               CCGCTC(-3/-3)
BsrDI         486  CATTGC               GCAATG(2/0)
BsrFI         117  GCCGGT               [GA]^CCGG[CT]
BssSI         779  CTCGTG               CACGAG(-5/-1)
BstXI         624  CCAAGTGTTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I         97  CCTAAGG              CC^T[ACGT]AGG
CelII         319  GCTGAGC              GC^T[ACGT]AGC
Cfr10I        117  GCCGGT               [GA]^CCGG[CT]
DraII          34  GGGTCCC              [GA]G^G[ACGT]CC[CT]
DraIII        163  CACATCGTG            CAC[ACGT][ACGT][ACGT]^GTG
EarI           87  GAAGAG               CTCTTC(1/4)
Eco81I         97  CCTAAGG              CC^T[ACGT]AGG
EcoO109I       34  GGGTCCC              [GA]G^G[ACGT]CC[CT]
FbaI          893  TGATCA               T^GATCA
FspI          743  TGCGCA               TGC^GCA
Hin1I         652  GACGTC               G[GA]^CG[CT]C
HincII        178  GTCGAC               GT[CT]^[GA]AC
HindII        178  GTCGAC               GT[CT]^[GA]AC
HinfI         159  GACTC                G^A[ACGT]TC
KpnI          612  GGTACC               GGTAC^C
Ksp632I        87  GAAGAG               CTCTTC(1/4)
MamI          540  GATGGAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI          296  TCCGGA               T^CCGGA
MseI         1094  TTAA                 T^TAA
MspA1I        433  CAGCTG               C[AC]G^C[GT]G
NciI          187  CCGGG                CC^[GC]GG
PaeR7I        287  CTCGAG               C^TCGAG
PflFI         985  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PleI          159  GACTC                GAGTC(4/5)
PpuMI          34  GGGTCCC              [GA]G^G[AT]CC[CT]
PshAI          29  GACATGGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII         433  CAGCTG               CAG^CTG
SalI          178  GTCGAC               G^TCGAC
SapI           87  GAAGAGC              GCTCTTC(1/4)
Tru9I        1094  TTAA                 T^TAA
Tth111I       985  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI          248  CCATGGTGGTTGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI          287  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AccII         592  CGCG                 CG^CG
              802  CGCG                
AspHI         322  GAGCAC               G[AT]GC[AT]^C
             1030  GTGCTC              
BalI          861  TGGCCA               TGG^CCA
             1122  TGGCCA              
BanII         587  GGGCCC               G[GA]GC[CT]^C
             1073  GGGCTC              
BpmI          436  CTGGAG               CTGGAG(16/14)
             1078  CTGGAG              
BsiHKAI       322  GAGCAC               G[AT]GC[AT]^C
             1030  GTGCTC              
BsmAI         238  GAGAC                GTCTC(1/5)
                3  GTCTC               
BsmFI        1167  GGGAC                GGGAC(10/14)
               36  GTCCC               
BsmI          337  GAATGC               GAATGC(1/-1)
             1159  GCATTC              
BsrI          584  ACTGG                ACTGG(1/-1)
              695  CCAGT               
BstEII        663  GGTGACC              G^GT[ACGT]ACC
              690  GGTCACC             
BstPI         663  GGTGACC              G^GT[ACGT]ACC
              690  GGTCACC             
BstUI         592  CGCG                 CG^CG
              802  CGCG                
BstYI         190  GGATCC               [GA]^GATC[CT]
              545  AGATCC              
EcoNI         741  CCTGCGCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              873  CCTTATCAAGG         
EcoO65I       663  GGTGACC              G^GT[ACGT]ACC
              690  GGTCACC             
MaeII         275  ACGT                 A^CGT
              653  ACGT                
MflI          190  GGATCC               [GA]^GATC[CT]
              545  AGATCC              
MluNI         861  TGGCCA               TGG^CCA
             1122  TGGCCA              
MscI          861  TGGCCA               TGG^CCA
             1122  TGGCCA              
MvnI          592  CGCG                 CG^CG
              802  CGCG                
ScaI          395  AGTACT               AGT^ACT
              464  AGTACT              
Tsp509I       361  AATT                 ^AATT
             1188  AATT                
XhoII         190  GGATCC               [GA]^GATC[CT]
              545  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   62 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        Asp700I     
AvaII       BbvI        BmyI        BsaJI       BsiYI       
BslI        Bsp1286I    BssKI       BstF5I      BstNI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DsaI        EaeI        EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeIII      HapII       HhaI        
HinP1I      HpaII       HphI        ItaI        MaeIII      
MboI        MboII       MnlI        MslI        MspI        
MvaI        MwoI        NcoI        NdeII       NlaIII      
NlaIV       PflMI       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SmlI        StuI        StyI        
TaqI        TseI        Tsp45I      TspRI       TthHB8I     
Van91I      XmnI        
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com