Restriction map for pF1KE0205
 (Circular) (Six-base) MAPSORT of: pF1KE0205.seq  Check: 3834  from: 1  to: 3755



pF1KE0205  3755 bp

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:11  ..

AccI GT'mk_AC

Cuts at:    793     793 
   Size:       3755
 
Acc65I G'GTAC_C

Cuts at:    771     771 
   Size:       3755
 
AclI AA'CG_TT

Cuts at:   3595    3595 
   Size:       3755
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    314     517    1580    2012    2759    3027     314 
   Size:        203    1063     432     747     268    1042

  Fragments arranged by size: 

                1063    1042     747     432     268     203
 
AflIII A'CryG_T

Cuts at:   1107    2211    3188    1107 
   Size:       1104     977    1674

  Fragments arranged by size: 

                1674    1104     977
 
AgeI A'CCGG_T

Cuts at:    134    2143     134 
   Size:       2009    1746
 
AlwNI CAG_nnn'CTG

Cuts at:   1060    2627    1060 
   Size:       1567    2188
 
ApaLI G'TGCA_C

Cuts at:   2525    2525 
   Size:       3755
 
ApoI r'AATT_y

Cuts at:    646     759    3108    3364     646 
   Size:        113    2349     256    1037

  Fragments arranged by size: 

                2349    1037     256     113
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3755
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3755
 
AvaI C'yCGr_G

Cuts at:    488     775     488 
   Size:        287    3468
 
BamHI G'GATC_C

Cuts at:    780     780 
   Size:       3755
 
BanI G'GyrC_C

Cuts at:    113     457     771    1185    1433    1468     113 
   Size:        344     314     414     248      35    2400

  Fragments arranged by size: 

                2400     414     344     314     248      35
 
BanII G_rGCy'C

Cuts at:    769    1799     769 
   Size:       1030    2725
 
BbeI G_GCGC'C

Cuts at:    117    1189    1437     117 
   Size:       1072     248    2435

  Fragments arranged by size: 

                2435    1072     248
 
BbsI GAAGACnn'nnnn_

Cuts at:    281     659     281 
   Size:        378    3377
 
BciVI GTATCCnnnnn_n'

Cuts at:   1653    2414    3755    1653 
   Size:        761    1341    1653

  Fragments arranged by size: 

                1653    1341     761
 
BclI T'GATC_A

Cuts at:   1275    1275 
   Size:       3755
 
BglI GCCn_nnn'nGGC

Cuts at:    304     304 
   Size:       3755
 
BglII A'GATC_T

Cuts at:   1270    3307    1270 
   Size:       2037    1718
 
BlpI GC'TnA_GC

Cuts at:    867     867 
   Size:       3755
 
Bme1580I G_kGCm'C

Cuts at:   1380    1473    2529    1380 
   Size:         93    1056    2606

  Fragments arranged by size: 

                2606    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    704     967    1048    1383    3527     704 
   Size:        263      81     335    2144     932

  Fragments arranged by size: 

                2144     932     335     263      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    394    2122     394 
   Size:       1728    2027
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    322     424     322 
   Size:        102    3653
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    931    1810    2302    2600     931 
   Size:        879     492     298    2086

  Fragments arranged by size: 

                2086     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3459    3459 
   Size:       3755
 
BsaAI yAC'GTr

Cuts at:   1108    1108 
   Size:       3755
 
BsaBI GATnn'nnATC

Cuts at:      6     202     604    1269    3306       6 
   Size:        196     402     665    2037     455

  Fragments arranged by size: 

                2037     665     455     402     196
 
BsaHI Gr'CG_yC

Cuts at:    114     591    1186    1434    2136    3632     114 
   Size:        477     595     248     702    1496     237

  Fragments arranged by size: 

                1496     702     595     477     248     237
 
BsaWI w'CCGG_w

Cuts at:    134     944    1465    2143    2417    2564    2975     134 
   Size:        810     521     678     274     147     411     914

  Fragments arranged by size: 

                 914     810     678     521     411     274     147
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    556     556 
   Size:       3755
 
BseYI C'CCAG_C

Cuts at:    424    1180    1938    2515    3011     424 
   Size:        756     758     577     496    1168

  Fragments arranged by size: 

                1168     758     756     577     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    727     727 
   Size:       3755
 
BsiEI CG_ry'CG

Cuts at:    104    1297    1343    2551     104 
   Size:       1193      46    1208    1308

  Fragments arranged by size: 

                1308    1208    1193      46
 
BsiHKAI G_wGCw'C

Cuts at:    769    1547    1737    2529     769 
   Size:        778     190     792    1995

  Fragments arranged by size: 

                1995     792     778     190
 
Bsp1286I G_dGCh'C

Cuts at:    769    1380    1473    1547    1737    1799    2529     769 
   Size:        611      93      74     190      62     730    1995

  Fragments arranged by size: 

                1995     730     611     190      93      74      62
 
BspEI T'CCGG_A

Cuts at:   2975    2975 
   Size:       3755
 
BspHI T'CATG_A

Cuts at:   2931    2931 
   Size:       3755
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    805    1019    1321    1702     805 
   Size:        214     302     381    2858

  Fragments arranged by size: 

                2858     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2047    2101    3585    3750      50 
   Size:       1997      54    1484     165      55

  Fragments arranged by size: 

                1997    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1667    1667 
   Size:       3755
 
BsrFI r'CCGG_y

Cuts at:    134    1753    1934    2143     134 
   Size:       1619     181     209    1746

  Fragments arranged by size: 

                1746    1619     209     181
 
BssSI C'ACGA_G

Cuts at:   2026    2384    2026 
   Size:        358    3397
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1132    1132 
   Size:       3755
 
BstBI TT'CG_AA

Cuts at:   2116    2116 
   Size:       3755
 
BstYI r'GATC_y

Cuts at:      1     197     780    1247    1270    1605    1851    2863    2949 
   Size:        196     583     467      23     335     246    1012      86

Cuts at:   2949    2961    3307       1 
   Size:         12     346     449

  Fragments arranged by size: 

                1012     583     467     449     346     335     246     196
                  86      23      12
 
BtgI C'CryG_G

Cuts at:    107     107 
   Size:       3755
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1705    1886    2049    1705 
   Size:        181     163    3411

  Fragments arranged by size: 

                3411     181     163
 
BtsI GCAGTG_nn'

Cuts at:    998     998 
   Size:       3755
 
DraI TTT'AAA

Cuts at:    754     754 
   Size:       3755
 
DraIII CAC_nnn'GTG

Cuts at:    253     264     253 
   Size:         11    3744
 
DrdI GACnn_nn'nnGTC

Cuts at:   1461    2319    1461 
   Size:        858    2897
 
EaeI y'GGCC_r

Cuts at:    255     662    1340    1514    1905    1932    3277     255 
   Size:        407     678     174     391      27    1345     733

  Fragments arranged by size: 

                1345     733     678     407     391     174      27
 
EagI C'GGCC_G

Cuts at:   1340    1340 
   Size:       3755
 
EarI CTCTTCn'nnn_

Cuts at:   1778    1988    1778 
   Size:        210    3545
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1963    2273    2419    3565    1963 
   Size:        310     146    1146    2153

  Fragments arranged by size: 

                2153    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    767     767 
   Size:       3755
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    314     322     424     517    1580    2012    2759    3027     314 
   Size:          8     102      93    1063     432     747     268    1042

  Fragments arranged by size: 

                1063    1042     747     432     268     102      93       8
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:    564     564 
   Size:       3755
 
EcoO109I rG'GnC_Cy

Cuts at:    894     894 
   Size:       3755
 
EcoRI G'AATT_C

Cuts at:    759     759 
   Size:       3755
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    639    1774     639 
   Size:       1135    2620
 
FspI TGC'GCA

Cuts at:    565    1536     565 
   Size:        971    2784
 
HaeII r_GCGC'y

Cuts at:    117    1189    1437    2459    3438     117 
   Size:       1072     248    1022     979     434

  Fragments arranged by size: 

                1072    1022     979     434     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    271     303    1597    1627    1629    1659     271 
   Size:         32    1294      30       2      30    2367

  Fragments arranged by size: 

                2367    1294      32      30      30       2
 
HincII GTy'rAC

Cuts at:    794     794 
   Size:       3755
 
HindIII A'AGCT_T

Cuts at:    529     529 
   Size:       3755
 
KasI G'GCGC_C

Cuts at:    113    1185    1433     113 
   Size:       1072     248    2435

  Fragments arranged by size: 

                2435    1072     248
 
KpnI G_GTAC'C

Cuts at:    775     775 
   Size:       3755
 
MluI A'CGCG_T

Cuts at:   3188    3188 
   Size:       3755
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2426    2610    2426 
   Size:        184    3571
 
MscI TGG'CCA

Cuts at:    257     664    1516     257 
   Size:        407     852    2496

  Fragments arranged by size: 

                2496     852     407
 
MslI CAynn'nnrTG

Cuts at:    370    1744    1871     370 
   Size:       1374     127    2254

  Fragments arranged by size: 

                2254    1374     127
 
MspA1I CmG'CkG

Cuts at:    285     866    1180    1540    2553    2798     285 
   Size:        581     314     360    1013     245    1242

  Fragments arranged by size: 

                1242    1013     581     360     314     245
 
NaeI GCC'GGC

Cuts at:   1936    1936 
   Size:       3755
 
NarI GG'CG_CC

Cuts at:    114    1186    1434     114 
   Size:       1072     248    2435

  Fragments arranged by size: 

                2435    1072     248
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       3755
 
NgoMIV G'CCGG_C

Cuts at:   1934    1934 
   Size:       3755
 
NspI r_CATG'y

Cuts at:    369     550     808    1308    2215     369 
   Size:        181     258     500     907    1909

  Fragments arranged by size: 

                1909     907     500     258     181
 
PasI CC'CwG_GG

Cuts at:    474     474 
   Size:       3755
 
PciI A'CATG_T

Cuts at:   2211    2211 
   Size:       3755
 
PflMI CCAn_nnn'nTGG

Cuts at:    253     617     253 
   Size:        364    3391
 
PmeI GTTT'AAAC

Cuts at:    754     754 
   Size:       3755
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2894    2926    2894 
   Size:         32    3723
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:    139     171     139 
   Size:         32    3723
 
PstI C_TGCA'G

Cuts at:    802    1487     802 
   Size:        685    3070
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3755
 
PvuII CAG'CTG

Cuts at:    285    1180    1540     285 
   Size:        895     360    2500

  Fragments arranged by size: 

                2500     895     360
 
RsrII CG'GwC_CG

Cuts at:   1950    1950 
   Size:       3755
 
SacI G_AGCT'C

Cuts at:    769     769 
   Size:       3755
 
SalI G'TCGA_C

Cuts at:    792     792 
   Size:       3755
 
SapI GCTCTTCn'nnn_

Cuts at:   1778    1988    1778 
   Size:        210    3545
 
SbfI CC_TGCA'GG

Cuts at:    802     802 
   Size:       3755
 
ScaI AGT'ACT

Cuts at:   3272    3272 
   Size:       3755
 
SfcI C'TryA_G

Cuts at:     33     482     798    1483    2476    2667      33 
   Size:        449     316     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     449     316     191
 
SfoI GGC'GCC

Cuts at:    115    1187    1435     115 
   Size:       1072     248    2435

  Fragments arranged by size: 

                2435    1072     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3755
 
SmaI CCC'GGG

Cuts at:    490     777     490 
   Size:        287    3468
 
SmlI C'TyrA_G

Cuts at:    910    1825    2317    2579     910 
   Size:        915     492     262    2086

  Fragments arranged by size: 

                2086     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3755
 
SphI G_CATG'C

Cuts at:    808    1308     808 
   Size:        500    3255
 
StyI C'CwwG_G

Cuts at:    107     722     889    1243     107 
   Size:        615     167     354    2619

  Fragments arranged by size: 

                2619     615     354     167
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1338    1471    1930    2139    1338 
   Size:        133     459     209    2954

  Fragments arranged by size: 

                2954     459     209     133
 
TatI w'GTAC_w

Cuts at:   3270    3270 
   Size:       3755
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3272    3304    3272 
   Size:         32    3723
 
Tth111I GACn'n_nGTC

Cuts at:   1552    1552 
   Size:       3755
 
XbaI T'CTAG_A

Cuts at:    786     786 
   Size:       3755
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    330    1250     330 
   Size:        920    2835
 
XmaI C'CCGG_G

Cuts at:    488     775     488 
   Size:        287    3468
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI    ApaLI
     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbeI
     BbsI    BciVI     BclI     BglI    BglII     BlpI Bme1580I     BmrI
     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI    BstYI
     BtgI    BtgZI     BtsI     DraI   DraIII     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI
     FspI    HaeII    Hin4I   HincII  HindIII     KasI     KpnI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV
     NspI     PasI     PciI    PflMI     PmeI     PpiI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI     SalI     SapI     SbfI     ScaI
     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI
     ApaI     AscI    AvrII     BaeI    BbvCI     BcgI    BfrBI    BmgBI
     BmtI   Bpu10I    BsaXI    BsiWI     BsmI    BsmBI    BsrGI   BssHII
   BstEII    BstXI  BstZ17I   Bsu36I     ClaI    CspCI    EcoRV     FseI
    FspAI     HpaI     MfeI     NdeI     NheI     NotI     NruI     NsiI
     PacI     PfoI     PmlI    PpuMI    PshAI     PsiI   PspOMI    PspXI
    SacII    SanDI    SexAI     SfiI    SgrAI    SnaBI     SrfI     SspI
     StuI     SwaI     XhoI     XmnI     ZraI
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