Restriction map for pF1KE0205
 (Linear) (Six-base) MAPSORT of: KIEE0205.seq  Check: 9191  from: 1  to: 642



KIEE0205.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:11  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     206     409     642 
   Size:        206     203     233

  Fragments arranged by size: 

                 233     206     203
 
AgeI A'CCGG_T

Cuts at:      0      26     642 
   Size:         26     616
 
ApoI r'AATT_y

Cuts at:      0     538     642 
   Size:        538     104
 
AvaI C'yCGr_G

Cuts at:      0     380     642 
   Size:        380     262
 
BanI G'GyrC_C

Cuts at:      0       5     349     642 
   Size:          5     344     293

  Fragments arranged by size: 

                 344     293       5
 
BbeI G_GCGC'C

Cuts at:      0       9     642 
   Size:          9     633
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     173     551     642 
   Size:        173     378      91

  Fragments arranged by size: 

                 378     173      91
 
BglI GCCn_nnn'nGGC

Cuts at:      0     196     642 
   Size:        196     446
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     596     642 
   Size:        596      46
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     286     642 
   Size:        286     356
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     214     316     642 
   Size:        214     102     326

  Fragments arranged by size: 

                 326     214     102
 
BsaBI GATnn'nnATC

Cuts at:      0      94     496     642 
   Size:         94     402     146

  Fragments arranged by size: 

                 402     146      94
 
BsaHI Gr'CG_yC

Cuts at:      0       6     483     642 
   Size:          6     477     159

  Fragments arranged by size: 

                 477     159       6
 
BsaWI w'CCGG_w

Cuts at:      0      26     642 
   Size:         26     616
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     448     642 
   Size:        448     194
 
BseYI C'CCAG_C

Cuts at:      0     316     642 
   Size:        316     326
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     619     642 
   Size:        619      23
 
BsrFI r'CCGG_y

Cuts at:      0      26     642 
   Size:         26     616
 
BstYI r'GATC_y

Cuts at:      0      89     642 
   Size:         89     553
 
DraIII CAC_nnn'GTG

Cuts at:      0     145     156     642 
   Size:        145      11     486

  Fragments arranged by size: 

                 486     145      11
 
EaeI y'GGCC_r

Cuts at:      0     147     554     642 
   Size:        147     407      88

  Fragments arranged by size: 

                 407     147      88
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     206     214     316     409     642 
   Size:        206       8     102      93     233

  Fragments arranged by size: 

                 233     206     102      93       8
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     456     642 
   Size:        456     186
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     531     642 
   Size:        531     111
 
FspI TGC'GCA

Cuts at:      0     457     642 
   Size:        457     185
 
HaeII r_GCGC'y

Cuts at:      0       9     642 
   Size:          9     633
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     163     195     642 
   Size:        163      32     447

  Fragments arranged by size: 

                 447     163      32
 
HindIII A'AGCT_T

Cuts at:      0     421     642 
   Size:        421     221
 
KasI G'GCGC_C

Cuts at:      0       5     642 
   Size:          5     637
 
MscI TGG'CCA

Cuts at:      0     149     556     642 
   Size:        149     407      86

  Fragments arranged by size: 

                 407     149      86
 
MslI CAynn'nnrTG

Cuts at:      0     262     642 
   Size:        262     380
 
MspA1I CmG'CkG

Cuts at:      0     177     642 
   Size:        177     465
 
NarI GG'CG_CC

Cuts at:      0       6     642 
   Size:          6     636
 
NspI r_CATG'y

Cuts at:      0     261     442     642 
   Size:        261     181     200

  Fragments arranged by size: 

                 261     200     181
 
PasI CC'CwG_GG

Cuts at:      0     366     642 
   Size:        366     276
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     145     509     642 
   Size:        145     364     133

  Fragments arranged by size: 

                 364     145     133
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0      31      63     642 
   Size:         31      32     579

  Fragments arranged by size: 

                 579      32      31
 
PvuII CAG'CTG

Cuts at:      0     177     642 
   Size:        177     465
 
SfcI C'TryA_G

Cuts at:      0     374     642 
   Size:        374     268
 
SfoI GGC'GCC

Cuts at:      0       7     642 
   Size:          7     635
 
SmaI CCC'GGG

Cuts at:      0     382     642 
   Size:        382     260
 
StyI C'CwwG_G

Cuts at:      0     614     642 
   Size:        614      28
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     222     642 
   Size:        222     420
 
XmaI C'CCGG_G

Cuts at:      0     380     642 
   Size:        380     262
 

 Enzymes that do cut:

     AcuI     AgeI     ApoI     AvaI     BanI     BbeI     BbsI     BglI
     BmrI     BplI     BpmI    BsaBI    BsaHI    BsaWI    BseRI    BseYI
     BsgI    BsrFI    BstYI   DraIII     EaeI  Eco57MI    EcoNI     FalI
     FspI    HaeII    Hin4I  HindIII     KasI     MscI     MslI   MspA1I
     NarI     NspI     PasI    PflMI     PsrI    PvuII     SfcI     SfoI
     SmaI     StyI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI    BanII    BbvCI     BcgI
    BciVI     BclI    BfrBI    BglII     BlpI Bme1580I    BmgBI     BmtI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaXI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I
   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI
     EagI     EarI     EciI  EcoICRI EcoO109I    EcoRI    EcoRV     FseI
    FspAI   HincII     HpaI     KpnI     MfeI     MluI     MmeI     NaeI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PstI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SgfI    SgrAI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
    TaqII     TatI     TstI  Tth111I     XbaI     XhoI     XmnI     ZraI
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