Restriction map for pF1KB8446
##################################
# NO CUT ENZYMES (  146 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AfaI        AflIII      AgeI        AhdI        
Alw44I      AocI        Aor51HI     ApaLI       AscI        
AseI        AsnI        Asp718I     AspEI       AspI        
AvaI        AvrII       BamHI       BanI        BbeI        
BbrPI       BbsI        BcgI        BciVI       BclI        
BglI        BlnI        BlpI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BsaHI       BseAI       BseRI       
BsgI        BsiEI       BsiWI       BsmBI       BsoBI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrDI       BsrGI       BssHII      BssSI       Bst1107I    
BstBI       BstEII      BstF5I      BstPI       BstZ17I     
Bsu36I      CelII       ClaI        CpoI        DrdI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoO65I     EcoRI       EcoT22I     
FbaI        FokI        FseI        HgaI        Hin1I       
HincII      HindII      HindIII     HpaI        KasI        
KpnI        Ksp632I     KspI        MaeII       MaeIII      
MamI        MfeI        MluI        MroI        MslI        
MunI        NaeI        NarI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsaI        
RsrII       SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
Tsp45I      Tth111I     XbaI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AflII         148  CTTAAG               C^TTAAG
AlwI          645  GATCC                GGATC(4/5)
AlwNI         391  CAGAAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI          586  GGGCCC               GGGCC^C
AspHI         803  GAGCTC               G[AT]GC[AT]^C
BalI          830  TGGCCA               TGG^CCA
BcnI          167  CCCGG                CC^[GC]GG
BfrI          148  CTTAAG               C^TTAAG
BglII         156  AGATCT               A^GATCT
BsaI          710  GAGACC               GGTCTC(1/5)
BsaWI          38  ACCGGA               [AT]^CCGG[AT]
BsiHKAI       803  GAGCTC               G[AT]GC[AT]^C
BsmI          206  GAATGC               GAATGC(1/-1)
BsrBI         766  GAGCGG               CCGCTC(-3/-3)
BsrFI         725  ACCGGC               [GA]^CCGG[CT]
BsrI          719  ACTGG                ACTGG(1/-1)
BstXI         575  CCAGCTCCTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I        725  ACCGGC               [GA]^CCGG[CT]
DraI          231  TTTAAA               TTT^AAA
DraIII        592  CACCGCGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI          887  CCACGG               C^C[GA][CT]GG
EaeI          830  TGGCCA               [CT]^GGCC[GA]
EcoNI         828  CCTGGCCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV         198  GATATC               GAT^ATC
FauI          561  CCCGC                CCCGC(4/6)
HaeII         701  AGCGCC               [GA]GCGC^[CT]
HphI          524  TCACC                GGTGA(8/7)
MluNI         830  TGGCCA               TGG^CCA
MscI          830  TGGCCA               TGG^CCA
NciI          167  CCCGG                CC^[GC]GG
PleI          463  GAGTC                GAGTC(4/5)
PpuMI         542  GGGACCT              [GA]G^G[AT]CC[CT]
PstI          361  CTGCAG               CTGCA^G
PvuII         634  CAGCTG               CAG^CTG
SacI          803  GAGCTC               GAGCT^C
SfaNI         427  GCATC                GCATC(5/9)
SmlI          148  CTTAAG               C^T[CT][GA]AG
TfiI          735  GATTC                G^A[AT]TC
XcmI          458  CCATCGAGTCAGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

Asp700I       249  GAAGAAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              435  GAAAGACTTC          
AviII         331  TGCGCA               TGC^GCA
              797  TGCGCA              
BbvCI         822  CCTCAGC              CCTCAGC(-5/-2)
              681  GCTGAGG             
BfaI          142  CTAG                 C^TAG
              652  CTAG                
BspMI         545  ACCTGC               ACCTGC(4/8)
              794  ACCTGC              
BstAPI        418  GCAGTTTGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              840  GCAGCATTTGC         
BstYI         156  AGATCT               [GA]^GATC[CT]
              644  AGATCC              
EcoT14I       581  CCTTGG               C^C[AT][AT]GG
              833  CCAAGG              
FspI          331  TGCGCA               TGC^GCA
              797  TGCGCA              
HinfI         463  GAGTC                G^A[ACGT]TC
              735  GATTC               
MaeI          142  CTAG                 C^TAG
              652  CTAG                
MflI          156  AGATCT               [GA]^GATC[CT]
              644  AGATCC              
MspA1I        609  CCGCTG               C[AC]G^C[GT]G
              634  CAGCTG              
PflMI         390  CCAGAAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              591  CCACCGCGTGG         
SfcI           66  CTGTAG               C^T[GA][CT]AG
              361  CTGCAG              
StyI          581  CCTTGG               C^C[AT][AT]GG
              833  CCAAGG              
TaqI          461  TCGA                 T^CGA
              924  TCGA                
TspRI         467  CAGTG                CA[GC]TG[ACGT][ACGT]^
              775  CAGTG               
TthHB8I       461  TCGA                 T^CGA
              924  TCGA                
Van91I        390  CCAGAAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              591  CCACCGCGTGG         
XhoII         156  AGATCT               [GA]^GATC[CT]
              644  AGATCC              
XmnI          249  GAAGAAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              435  GAAAGACTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   51 enzymes) #
#########################################

AccII       AciI        AcsI        AluI        ApoI        
AvaII       BanII       BbvI        BmyI        BpmI        
BsaJI       BsiYI       BslI        BsmAI       BsmFI       
Bsp1286I    BssKI       BstNI       BstUI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraII       
EcoO109I    EcoRII      Fnu4HI      HaeIII      HapII       
HhaI        HinP1I      HpaII       ItaI        MboI        
MboII       MnlI        MseI        MspI        MvaI        
MvnI        MwoI        NdeII       NlaIII      NlaIV       
Sau3AI      Sau96I      ScrFI       Tru9I       TseI        
Tsp509I     
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com