Restriction map for pF1KB8446
 (Linear) (Six-base) MAPSORT of: KIBB8446.seq  Check: 8002  from: 1  to: 927



KIBB8446.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:28  ..

AflII C'TTAA_G

Cuts at:      0     148     927 
   Size:        148     779
 
AlwNI CAG_nnn'CTG

Cuts at:      0     396     927 
   Size:        396     531
 
ApaI G_GGCC'C

Cuts at:      0     590     927 
   Size:        590     337
 
ApoI r'AATT_y

Cuts at:      0     121     214     253     927 
   Size:        121      93      39     674

  Fragments arranged by size: 

                 674     121      93      39
 
BanII G_rGCy'C

Cuts at:      0     590     747     807     927 
   Size:        590     157      60     120

  Fragments arranged by size: 

                 590     157     120      60
 
BbvCI CC'TCA_GC

Cuts at:      0     682     823     927 
   Size:        682     141     104

  Fragments arranged by size: 

                 682     141     104
 
BglII A'GATC_T

Cuts at:      0     156     927 
   Size:        156     771
 
Bme1580I G_kGCm'C

Cuts at:      0     590     927 
   Size:        590     337
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     729     927 
   Size:        729     198
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     373     658     673     927 
   Size:        373     285      15     254

  Fragments arranged by size: 

                 373     285     254      15
 
Bpu10I CC'TnA_GC

Cuts at:      0     682     823     927 
   Size:        682     141     104

  Fragments arranged by size: 

                 682     141     104
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     704     927 
   Size:        704     223
 
BsaWI w'CCGG_w

Cuts at:      0      38     927 
   Size:         38     889
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     535     565     927 
   Size:        535      30     362

  Fragments arranged by size: 

                 535     362      30
 
BsiHKAI G_wGCw'C

Cuts at:      0     807     927 
   Size:        807     120
 
BsmI GAATG_Cn'

Cuts at:      0     212     927 
   Size:        212     715
 
Bsp1286I G_dGCh'C

Cuts at:      0     590     747     807     927 
   Size:        590     157      60     120

  Fragments arranged by size: 

                 590     157     120      60
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     554     803     927 
   Size:        554     249     124

  Fragments arranged by size: 

                 554     249     124
 
BsrBI CCG'CTC

Cuts at:      0     768     927 
   Size:        768     159
 
BsrFI r'CCGG_y

Cuts at:      0     725     927 
   Size:        725     202
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     424     846     927 
   Size:        424     422      81

  Fragments arranged by size: 

                 424     422      81
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     582     927 
   Size:        582     345
 
BstYI r'GATC_y

Cuts at:      0     156     644     927 
   Size:        156     488     283

  Fragments arranged by size: 

                 488     283     156
 
BtgI C'CryG_G

Cuts at:      0     887     927 
   Size:        887      40
 
BtsI GCAGTG_nn'

Cuts at:      0     781     927 
   Size:        781     146
 
DraI TTT'AAA

Cuts at:      0     233     927 
   Size:        233     694
 
DraIII CAC_nnn'GTG

Cuts at:      0     597     927 
   Size:        597     330
 
EaeI y'GGCC_r

Cuts at:      0     830     927 
   Size:        830      97
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     637     744     927 
   Size:        637     107     183

  Fragments arranged by size: 

                 637     183     107
 
EcoICRI GAG'CTC

Cuts at:      0     805     927 
   Size:        805     122
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     373     658     673     927 
   Size:        373     285      15     254

  Fragments arranged by size: 

                 373     285     254      15
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     832     927 
   Size:        832      95
 
EcoO109I rG'GnC_Cy

Cuts at:      0     339     543     586     587     927 
   Size:        339     204      43       1     340

  Fragments arranged by size: 

                 340     339     204      43       1
 
EcoRV GAT'ATC

Cuts at:      0     200     927 
   Size:        200     727
 
FspI TGC'GCA

Cuts at:      0     333     799     927 
   Size:        333     466     128

  Fragments arranged by size: 

                 466     333     128
 
HaeII r_GCGC'y

Cuts at:      0     705     927 
   Size:        705     222
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     535     567     927 
   Size:        535      32     360

  Fragments arranged by size: 

                 535     360      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     111     158     927 
   Size:        111      47     769

  Fragments arranged by size: 

                 769     111      47
 
MscI TGG'CCA

Cuts at:      0     832     927 
   Size:        832      95
 
MspA1I CmG'CkG

Cuts at:      0     611     636     927 
   Size:        611      25     291

  Fragments arranged by size: 

                 611     291      25
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     396     597     927 
   Size:        396     201     330

  Fragments arranged by size: 

                 396     330     201
 
PfoI T'CCnGG_A

Cuts at:      0     671     689     927 
   Size:        671      18     238

  Fragments arranged by size: 

                 671     238      18
 
PpuMI rG'GwC_Cy

Cuts at:      0     543     927 
   Size:        543     384
 
PspOMI G'GGCC_C

Cuts at:      0     586     927 
   Size:        586     341
 
PstI C_TGCA'G

Cuts at:      0     365     927 
   Size:        365     562
 
PvuII CAG'CTG

Cuts at:      0     636     927 
   Size:        636     291
 
SacI G_AGCT'C

Cuts at:      0     807     927 
   Size:        807     120
 
SfcI C'TryA_G

Cuts at:      0      66     361     927 
   Size:         66     295     566

  Fragments arranged by size: 

                 566     295      66
 
SmlI C'TyrA_G

Cuts at:      0     148     927 
   Size:        148     779
 
StyI C'CwwG_G

Cuts at:      0     581     833     927 
   Size:        581     252      94

  Fragments arranged by size: 

                 581     252      94
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     465     927 
   Size:        465     462
 
XmnI GAAnn'nnTTC

Cuts at:      0     253     439     927 
   Size:        253     186     488

  Fragments arranged by size: 

                 488     253     186
 

 Enzymes that do cut:

    AflII    AlwNI     ApaI     ApoI    BanII    BbvCI    BglII Bme1580I
     BmrI     BpmI   Bpu10I     BsaI    BsaWI    BsaXI  BsiHKAI     BsmI
 Bsp1286I    BspMI    BsrBI    BsrFI   BstAPI    BstXI    BstYI     BtgI
     BtsI     DraI   DraIII     EaeI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRV     FspI    HaeII    Hin4I     MmeI     MscI   MspA1I
    PflMI     PfoI    PpuMI   PspOMI     PstI    PvuII     SacI     SfcI
     SmlI     StyI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI     BbsI
     BcgI    BciVI     BclI    BfrBI     BglI     BlpI    BmgBI     BmtI
     BplI    BpuEI    BsaAI    BsaBI    BsaHI    BseRI    BseYI     BsgI
    BsiEI    BsiWI    BsmBI    BspEI    BspHI    BsrDI    BsrGI   BssHII
    BssSI    BstBI   BstEII  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI
     DrdI     EagI     EarI    EcoRI     FalI     FseI    FspAI   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MslI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI     PmeI     PmlI     PpiI    PshAI
     PsiI    PspXI     PsrI     PvuI    RsrII    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
    TaqII     TatI     TstI  Tth111I     XbaI     XhoI     XmaI     ZraI
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