Restriction map for pF1KB7855
##################################
# NO CUT ENZYMES (   98 enzymes) #
##################################

AatII       Acc65I      AclI        AcyI        AflII       
AflIII      AgeI        AhdI        AlwI        Aor51HI     
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AvrII       BamHI       BanI        
BbeI        BbrPI       BbvCI       BciVI       BclI        
BfrI        BlnI        BsaAI       BsaBI       BsaHI       
BsiWI       BsmBI       Bsp1407I    BspDI       BspLU11I    
BsrBI       BsrGI       BssHII      Bst1107I    BstAPI      
BstBI       BstZ17I     ClaI        DraI        DraIII      
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoRV       EcoT22I     FbaI        FseI        
Hin1I       HindIII     HpaI        KasI        KpnI        
MamI        MfeI        MluI        MseI        MunI        
NaeI        NarI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
PshBI       Psp1406I    ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SnaBI       SpeI        
SspBI       SspI        SwaI        Tru9I       Tth111I     
XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   57 enzymes) #
###################################

AccI         1405  GTCGAC               GT^[AC][GT]AC
AccII         126  CGCG                 CG^CG
AccIII        755  TCCGGA               T^CCGGA
AcsI          853  GAATTC               [GA]^AATT[CT]
Alw44I        730  GTGCAC               G^TGCAC
ApaLI         730  GTGCAC               G^TGCAC
ApoI          853  GAATTC               [GA]^AATT[CT]
AviII         215  TGCGCA               TGC^GCA
BalI          743  TGGCCA               TGG^CCA
BglI          284  GCCGGGATGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI          919  GAGACC               GGTCTC(1/5)
BseAI         755  TCCGGA               T^CCGGA
BsgI          655  GTGCAG               GTGCAG(16/14)
BsmI          462  GAATGC               GAATGC(1/-1)
BspEI         755  TCCGGA               T^CCGGA
BspHI         935  TCATGA               T^CATGA
BsrFI         248  ACCGGC               [GA]^CCGG[CT]
BssSI         262  CACGAG               CACGAG(-5/-1)
BstEII        228  GGTCACC              G^GT[ACGT]ACC
BstPI         228  GGTCACC              G^GT[ACGT]ACC
BstUI         126  CGCG                 CG^CG
Cfr10I        248  ACCGGC               [GA]^CCGG[CT]
CpoI          317  CGGACCG              CG^G[AT]CCG
EaeI          743  TGGCCA               [CT]^GGCC[GA]
EcoO65I       228  GGTCACC              G^GT[ACGT]ACC
EcoRI         853  GAATTC               G^AATTC
FspI          215  TGCGCA               TGC^GCA
HaeII         119  GGCGCT               [GA]GCGC^[CT]
HgaI          986  GACGC                GACGC(5/10)
HincII       1405  GTCGAC               GT[CT]^[GA]AC
HindII       1405  GTCGAC               GT[CT]^[GA]AC
KspI          125  CCGCGG               CCGC^GG
MluNI         743  TGGCCA               TGG^CCA
MroI          755  TCCGGA               T^CCGGA
MscI          743  TGGCCA               TGG^CCA
MvnI          126  CGCG                 CG^CG
NdeI          914  CATATG               CA^TATG
NspI           92  GCATGC               [GA]CATG^[CT]
PflMI         339  CCAGAACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI         877  GACGATCGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI          879  CGATCG               CGAT^CG
RcaI          935  TCATGA               T^CATGA
RsrII         317  CGGACCG              CG^G[AT]CCG
SacII         125  CCGCGG               CCGC^GG
SalI         1405  GTCGAC               G^TCGAC
SapI          436  GAAGAGC              GCTCTTC(1/4)
SbfI          783  CCTGCAGG             CCTGCA^GG
SmaI         1560  CCCGGG               CCC^GGG
SmlI          580  CTCAAG               C^T[CT][GA]AG
SphI           92  GCATGC               GCATG^C
Sse8387I      783  CCTGCAGG             CCTGCA^GG
TfiI          350  GAATC                G^A[AT]TC
Tsp509I       854  AATT                 ^AATT
Van91I        339  CCAGAACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI         1607  CCATTGCGGACATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI        1560  CCCGGG               C^CCGGG
XmaI         1560  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   34 enzymes) #
###################################

AfaI          861  GTAC                 GT^AC
             1485  GTAC                
AocI         1252  CCTCAGG              CC^T[ACGT]AGG
             1489  CCTGAGG             
ApaI         1517  GGGCCC               GGGCC^C
             1556  GGGCCC              
AvaI          704  CCCGAG               C^[CT]CG[GA]G
             1560  CCCGGG              
BbsI         1342  GAAGAC               GAAGAC(2/6)
             1594  GAAGAC              
BcgI          456  CGACCTGAATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
              611  GCAGCTGCCTCG        
BfaI         1237  CTAG                 C^TAG
             1507  CTAG                
BglII         584  AGATCT               A^GATCT
              632  AGATCT              
BlpI          304  GCTGAGC              GC^T[ACGT]AGC
             1010  GCTCAGC             
Bpu1102I      304  GCTGAGC              GC^T[ACGT]AGC
             1010  GCTCAGC             
BpuAI        1342  GAAGAC               GAAGAC(2/6)
             1594  GAAGAC              
BsaWI         755  TCCGGA               [AT]^CCGG[AT]
              887  ACCGGA              
BsiEI         828  CGACCG               CG[GA][CT]^CG
              879  CGATCG              
BsmAI         919  GAGAC                GTCTC(1/5)
              802  GTCTC               
BsoBI         704  CCCGAG               C^[CT]CG[GA]G
             1560  CCCGGG              
BspMI         863  ACCTGC               ACCTGC(4/8)
             1269  ACCTGC              
BsrDI         990  CATTGC               GCAATG(2/0)
             1608  CATTGC              
BstXI          42  CCAGGCCTCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1472  CCATGCTGATGG        
BstYI         584  AGATCT               [GA]^GATC[CT]
              632  AGATCT              
Bsu36I       1252  CCTCAGG              CC^T[ACGT]AGG
             1489  CCTGAGG             
CelII         304  GCTGAGC              GC^T[ACGT]AGC
             1010  GCTCAGC             
EarI          436  GAAGAG               CTCTTC(1/4)
              942  GAAGAG              
Eco81I       1252  CCTCAGG              CC^T[ACGT]AGG
             1489  CCTGAGG             
EcoNI         375  CCTGGAGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1158  CCTGCCCCAGG         
Ksp632I       436  GAAGAG               CTCTTC(1/4)
              942  GAAGAG              
MaeI         1237  CTAG                 C^TAG
             1507  CTAG                
MaeII         903  ACGT                 A^CGT
             1054  ACGT                
MflI          584  AGATCT               [GA]^GATC[CT]
              632  AGATCT              
PleI          945  GAGTC                GAGTC(4/5)
             1638  GAGTC               
RsaI          861  GTAC                 GT^AC
             1485  GTAC                
StuI           44  AGGCCT               AGG^CCT
             1124  AGGCCT              
TaqI          984  TCGA                 T^CGA
             1406  TCGA                
TthHB8I       984  TCGA                 T^CGA
             1406  TCGA                
XhoII         584  AGATCT               [GA]^GATC[CT]
              632  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   69 enzymes) #
#########################################

AciI        AluI        AlwNI       AspHI       AvaII       
BanII       BbvI        BcnI        BmyI        BpmI        
BsaJI       BseRI       BsiHKAI     BsiYI       BslI        
BsmFI       Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraII       DsaI        EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeIII      
HapII       HhaI        HinP1I      HinfI       HpaII       
HphI        ItaI        MaeIII      MboI        MboII       
MnlI        MslI        MspA1I      MspI        MvaI        
MwoI        NciI        NcoI        NdeII       NlaIII      
NlaIV       PpuMI       PstI        PvuII       SacI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
StyI        TseI        Tsp45I      TspRI       
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