Restriction map for pF1KB7855
 (Circular) (Six-base) MAPSORT of: pF1KB7855.seq  Check: 1637  from: 1  to: 4766



pF1KB7855  4766 bp

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:50  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   1386    1386 
   Size:       4766
 
AccI GT'mk_AC

Cuts at:   1514    1804    1514 
   Size:        290    4476
 
Acc65I G'GTAC_C

Cuts at:   1782    1782 
   Size:       4766
 
AclI AA'CG_TT

Cuts at:   4606    4606 
   Size:       4766
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   2591    3023    3770    4038    2591 
   Size:        432     747     268    3319

  Fragments arranged by size: 

                3319     747     432     268
 
AflIII A'CryG_T

Cuts at:   2118    3222    4199    2118 
   Size:       1104     977    2685

  Fragments arranged by size: 

                2685    1104     977
 
AgeI A'CCGG_T

Cuts at:   3154    3154 
   Size:       4766
 
AleI CACnn'nnGTG

Cuts at:   1204    1204 
   Size:       4766
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1120    1120 
   Size:       4766
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    103     135     103 
   Size:         32    4734
 
AlwNI CAG_nnn'CTG

Cuts at:    453     972    1126    1285    1333    1536    1539    1651    2071 
   Size:        519     154     159      48     203       3     112     420

Cuts at:   2071    3638     453 
   Size:       1567    1581

  Fragments arranged by size: 

                1581    1567     519     420     203     159     154     112
                  48       3
 
ApaI G_GGCC'C

Cuts at:   1629    1668    1629 
   Size:         39    4727
 
ApaLI G'TGCA_C

Cuts at:    838    3536     838 
   Size:       2698    2068
 
ApoI r'AATT_y

Cuts at:    961    1770    4119    4375     961 
   Size:        809    2349     256    1352

  Fragments arranged by size: 

                2349    1352     809     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4766
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4766
 
AvaI C'yCGr_G

Cuts at:    812    1668    1786     812 
   Size:        856     118    3792

  Fragments arranged by size: 

                3792     856     118
 
BamHI G'GATC_C

Cuts at:   1791    1791 
   Size:       4766
 
BanI G'GyrC_C

Cuts at:   1782    2196    2444    2479    1782 
   Size:        414     248      35    4069

  Fragments arranged by size: 

                4069     414     248      35
 
BanII G_rGCy'C

Cuts at:    419     689     947     956    1580    1629    1668    1676    1780 
   Size:        270     258       9     624      49      39       8     104

Cuts at:   1780    2810     419 
   Size:       1030    2375

  Fragments arranged by size: 

                2375    1030     624     270     258     104      49      39
                   9       8
 
BbeI G_GCGC'C

Cuts at:   2200    2448    2200 
   Size:        248    4518
 
BbsI GAAGACnn'nnnn_

Cuts at:   1457    1709    1457 
   Size:        252    4514
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    553     587     708     742     553 
   Size:         34     121      34    4577

  Fragments arranged by size: 

                4577     121      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   2664    3425    4766    2664 
   Size:        761    1341    2664

  Fragments arranged by size: 

                2664    1341     761
 
BclI T'GATC_A

Cuts at:   2286    2286 
   Size:       4766
 
BglI GCCn_nnn'nGGC

Cuts at:    398     398 
   Size:       4766
 
BglII A'GATC_T

Cuts at:    692     740    2281    4318     692 
   Size:         48    1541    2037    1140

  Fragments arranged by size: 

                2037    1541    1140      48
 
BlpI GC'TnA_GC

Cuts at:    413    1119    1878     413 
   Size:        706     759    3301

  Fragments arranged by size: 

                3301     759     706
 
Bme1580I G_kGCm'C

Cuts at:    672     842    1629    1668    2391    2484    3540     672 
   Size:        170     787      39     723      93    1056    1898

  Fragments arranged by size: 

                1898    1056     787     723     170      93      39
 
BmrI ACTGGGnnnn_n'

Cuts at:   1333    1669    1978    2059    2394    4538    1333 
   Size:        336     309      81     335    2144    1561

  Fragments arranged by size: 

                2144    1561     336     335     309      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    246    3133     246 
   Size:       2887    1879
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    505    1262    1280     505 
   Size:        757      18    3991

  Fragments arranged by size: 

                3991     757      18
 
Bpu10I CC'TnA_GC

Cuts at:   1171    1171 
   Size:       4766
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    673    1942    2821    3313    3611     673 
   Size:       1269     879     492     298    1828

  Fragments arranged by size: 

                1828    1269     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   1021    4470    1021 
   Size:       3449    1317
 
BsaAI yAC'GTr

Cuts at:   2119    2119 
   Size:       4766
 
BsaBI GATnn'nnATC

Cuts at:      6    2280    4317       6 
   Size:       2274    2037     455

  Fragments arranged by size: 

                2274    2037     455
 
BsaHI Gr'CG_yC

Cuts at:   2197    2445    3147    4643    2197 
   Size:        248     702    1496    2320

  Fragments arranged by size: 

                2320    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:    863     995    1955    2476    3154    3428    3575    3986     863 
   Size:        132     960     521     678     274     147     411    1643

  Fragments arranged by size: 

                1643     960     678     521     411     274     147     132
            
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1696    1726    1696 
   Size:         30    4736
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    270     341    1018    1349    1701     270 
   Size:         71     677     331     352    3335

  Fragments arranged by size: 

                3335     677     352     331      71
 
BseYI C'CCAG_C

Cuts at:    803     886    1145    1326    1399    1485    1644    2191    2949 
   Size:         83     259     181      73      86     159     547     758

Cuts at:   2949    3526    4022     803 
   Size:        577     496    1547

  Fragments arranged by size: 

                1547     758     577     547     496     259     181     159
                  86      83      73
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    784     784 
   Size:       4766
 
BsiEI CG_ry'CG

Cuts at:    104     939     990    2308    2354    3562     104 
   Size:        835      51    1318      46    1208    1308

  Fragments arranged by size: 

                1318    1308    1208     835      51      46
 
BsiHKAI G_wGCw'C

Cuts at:    262     419     689     842     947    1177    1780    2558    2748 
   Size:        157     270     153     105     230     603     778     190

Cuts at:   2748    3540     262 
   Size:        792    1488

  Fragments arranged by size: 

                1488     792     778     603     270     230     190     157
                 153     105
 
BsmI GAATG_Cn'

Cuts at:    576     576 
   Size:       4766
 
Bsp1286I G_dGCh'C

Cuts at:    262     419     672     689     842     947     956    1177    1580 
   Size:        157     253      17     153     105       9     221     403

Cuts at:   1580    1629    1668    1676    1780    2391    2484    2558    2748 
   Size:         49      39       8     104     611      93      74     190

Cuts at:   2748    2810    3540     262 
   Size:         62     730    1488

  Fragments arranged by size: 

                1488     730     611     403     253     221     190     157
                 153     105     104      93      74      62      49      39
                  17       9       8
 
BspEI T'CCGG_A

Cuts at:    863    3986     863 
   Size:       3123    1643
 
BspHI T'CATG_A

Cuts at:   1043    3942    1043 
   Size:       2899    1867
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    980    1386    1816    2030    2332    2713     980 
   Size:        406     430     214     302     381    3033

  Fragments arranged by size: 

                3033     430     406     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    3058    3112    4596    4761      50 
   Size:       3008      54    1484     165      55

  Fragments arranged by size: 

                3008    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1097    1715    2678    1097 
   Size:        618     963    3185

  Fragments arranged by size: 

                3185     963     618
 
BsrFI r'CCGG_y

Cuts at:    356    2764    2945    3154     356 
   Size:       2408     181     209    1968

  Fragments arranged by size: 

                2408    1968     209     181
 
BssSI C'ACGA_G

Cuts at:    370    3037    3395     370 
   Size:       2667     358    1741

  Fragments arranged by size: 

                2667    1741     358
 
BstAPI GCAn_nnn'nTGC

Cuts at:   2143    2143 
   Size:       4766
 
BstBI TT'CG_AA

Cuts at:   3127    3127 
   Size:       4766
 
BstEII G'GTnAC_C

Cuts at:    336     336 
   Size:       4766
 
BstXI CCAn_nnnn'nTGG

Cuts at:    157    1587     157 
   Size:       1430    3336
 
BstYI r'GATC_y

Cuts at:      1     692     740    1791    2258    2281    2616    2862    3874 
   Size:        691      48    1051     467      23     335     246    1012

Cuts at:   3874    3960    3972    4318       1 
   Size:         86      12     346     449

  Fragments arranged by size: 

                1051    1012     691     467     449     346     335     246
                  86      48      23      12
 
Bsu36I CC'TnA_GG

Cuts at:   1361    1598    1361 
   Size:        237    4529
 
BtgI C'CryG_G

Cuts at:    107     233     956    1202    1301     107 
   Size:        126     723     246      99    3572

  Fragments arranged by size: 

                3572     723     246     126      99
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2716    2897    3060    2716 
   Size:        181     163    4422

  Fragments arranged by size: 

                4422     181     163
 
BtsI GCAGTG_nn'

Cuts at:    677    2009     677 
   Size:       1332    3434
 
DraI TTT'AAA

Cuts at:   1765    1765 
   Size:       4766
 
DrdI GACnn_nn'nnGTC

Cuts at:   2472    3330    2472 
   Size:        858    3908
 
EaeI y'GGCC_r

Cuts at:    851    2351    2525    2916    2943    4288     851 
   Size:       1500     174     391      27    1345    1329

  Fragments arranged by size: 

                1500    1345    1329     391     174      27
 
EagI C'GGCC_G

Cuts at:   2351    2351 
   Size:       4766
 
EarI CTCTTCn'nnn_

Cuts at:    539    1045    2789    2999     539 
   Size:        506    1744     210    2306

  Fragments arranged by size: 

                2306    1744     506     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    616    2974    3284    3430    4576     616 
   Size:       2358     310     146    1146     806

  Fragments arranged by size: 

                2358    1146     806     310     146
 
EcoICRI GAG'CTC

Cuts at:    417     687     945    1778     417 
   Size:        270     258     833    3405

  Fragments arranged by size: 

                3405     833     270     258
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    505    1262    1280    2591    3023    3770    4038     505 
   Size:        757      18    1311     432     747     268    1233

  Fragments arranged by size: 

                1311    1233     757     747     432     268      18
 
EcoNI CCTnn'n_nnAGG

Cuts at:    487    1270     487 
   Size:        783    3983
 
EcoO109I rG'GnC_Cy

Cuts at:    345     480     606     620    1323    1354    1422    1460    1625 
   Size:        135     126      14     703      31      68      38     165

Cuts at:   1625    1634    1664    1665    1905     345 
   Size:          9      30       1     240    3206

  Fragments arranged by size: 

                3206     703     240     165     135     126      68      38
                  31      30      14       9       1
 
EcoRI G'AATT_C

Cuts at:    961    1770     961 
   Size:        809    3957
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2785    2785 
   Size:       4766
 
FspI TGC'GCA

Cuts at:    325    2547     325 
   Size:       2222    2544
 
FspAI rTGC'GCAy

Cuts at:    325     325 
   Size:       4766
 
HaeII r_GCGC'y

Cuts at:    231    2200    2448    3470    4449     231 
   Size:       1969     248    1022     979     548

  Fragments arranged by size: 

                1969    1022     979     548     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1492    1524    1696    1728    2608    2638    2640    2670    1492 
   Size:         32     172      32     880      30       2      30    3588

  Fragments arranged by size: 

                3588     880     172      32      32      30      30       2
            
 
HincII GTy'rAC

Cuts at:   1515    1805    1515 
   Size:        290    4476
 
KasI G'GCGC_C

Cuts at:   2196    2444    2196 
   Size:        248    4518
 
KpnI G_GTAC'C

Cuts at:   1786    1786 
   Size:       4766
 
MluI A'CGCG_T

Cuts at:   4199    4199 
   Size:       4766
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    959    3437    3621     959 
   Size:       2478     184    2104

  Fragments arranged by size: 

                2478    2104     184
 
MscI TGG'CCA

Cuts at:    853    2527     853 
   Size:       1674    3092
 
MslI CAynn'nnrTG

Cuts at:    781    1102    1204    1555    1585    2755    2882     781 
   Size:        321     102     351      30    1170     127    2665

  Fragments arranged by size: 

                2665    1170     351     321     127     102      30
 
MspA1I CmG'CkG

Cuts at:    195     235     431     722     921    1064    1434    1489    1536 
   Size:         40     196     291     199     143     370      55      47

Cuts at:   1536    1877    2191    2551    3564    3809     195 
   Size:        341     314     360    1013     245    1152

  Fragments arranged by size: 

                1152    1013     370     360     341     314     291     245
                 199     196     143      55      47      40
 
NaeI GCC'GGC

Cuts at:   2947    2947 
   Size:       4766
 
NarI GG'CG_CC

Cuts at:   2197    2445    2197 
   Size:        248    4518
 
NcoI C'CATG_G

Cuts at:    107     956    1202    1301     107 
   Size:        849     246      99    3572

  Fragments arranged by size: 

                3572     849     246      99
 
NdeI CA'TA_TG

Cuts at:   1023    1023 
   Size:       4766
 
NgoMIV G'CCGG_C

Cuts at:   2945    2945 
   Size:       4766
 
NspI r_CATG'y

Cuts at:    204    1819    2319    3226     204 
   Size:       1615     500     907    1744

  Fragments arranged by size: 

                1744    1615     907     500
 
PciI A'CATG_T

Cuts at:   3222    3222 
   Size:       4766
 
PflMI CCAn_nnn'nTGG

Cuts at:    453     453 
   Size:       4766
 
PmeI GTTT'AAAC

Cuts at:   1765    1765 
   Size:       4766
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    697     729    3905    3937     697 
   Size:         32    3176      32    1526

  Fragments arranged by size: 

                3176    1526      32      32
 
PpuMI rG'GwC_Cy

Cuts at:    345     480     606     345 
   Size:        135     126    4505

  Fragments arranged by size: 

                4505     135     126
 
PshAI GACnn'nnGTC

Cuts at:    989     989 
   Size:       4766
 
PspOMI G'GGCC_C

Cuts at:   1625    1664    1625 
   Size:         39    4727
 
PstI C_TGCA'G

Cuts at:    896    1433    1439    1813    2498     896 
   Size:        537       6     374     685    3164

  Fragments arranged by size: 

                3164     685     537     374       6
 
PvuI CG_AT'CG

Cuts at:    104     990     104 
   Size:        886    3880
 
PvuII CAG'CTG

Cuts at:    722     921    1434    1489    1536    2191    2551     722 
   Size:        199     513      55      47     655     360    2937

  Fragments arranged by size: 

                2937     655     513     360     199      55      47
 
RsrII CG'GwC_CG

Cuts at:    426    2961     426 
   Size:       2535    2231
 
SacI G_AGCT'C

Cuts at:    419     689     947    1780     419 
   Size:        270     258     833    3405

  Fragments arranged by size: 

                3405     833     270     258
 
SacII CC_GC'GG

Cuts at:    236     236 
   Size:       4766
 
SalI G'TCGA_C

Cuts at:   1513    1803    1513 
   Size:        290    4476
 
SanDI GG'GwC_CC

Cuts at:    606     606 
   Size:       4766
 
SapI GCTCTTCn'nnn_

Cuts at:    539    2789    2999     539 
   Size:       2250     210    2306

  Fragments arranged by size: 

                2306    2250     210
 
SbfI CC_TGCA'GG

Cuts at:    896    1813     896 
   Size:        917    3849
 
ScaI AGT'ACT

Cuts at:   4283    4283 
   Size:       4766
 
SfcI C'TryA_G

Cuts at:     33     539     892    1429    1435    1809    2494    3487    3678 
   Size:        506     353     537       6     374     685     993     191

Cuts at:   3678      33 
   Size:       1121

  Fragments arranged by size: 

                1121     993     685     537     506     374     353     191
                   6
 
SfoI GGC'GCC

Cuts at:   2198    2446    2198 
   Size:        248    4518
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4766
 
SmaI CCC'GGG

Cuts at:   1670    1788    1670 
   Size:        118    4648
 
SmlI C'TyrA_G

Cuts at:    688    1921    2836    3328    3590     688 
   Size:       1233     915     492     262    1864

  Fragments arranged by size: 

                1864    1233     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4766
 
SphI G_CATG'C

Cuts at:    204    1819    2319     204 
   Size:       1615     500    2651

  Fragments arranged by size: 

                2651    1615     500
 
StuI AGG'CCT

Cuts at:    154    1234     154 
   Size:       1080    3686
 
StyI C'CwwG_G

Cuts at:    107     341     956    1202    1241    1301    1900    2254     107 
   Size:        234     615     246      39      60     599     354    2619

  Fragments arranged by size: 

                2619     615     599     354     246     234      60      39
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1408    2349    2482    2941    3150    1408 
   Size:        941     133     459     209    3024

  Fragments arranged by size: 

                3024     941     459     209     133
 
TatI w'GTAC_w

Cuts at:   4281    4281 
   Size:       4766
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   4283    4315    4283 
   Size:         32    4734
 
Tth111I GACn'n_nGTC

Cuts at:   2563    2563 
   Size:       4766
 
XbaI T'CTAG_A

Cuts at:   1797    1797 
   Size:       4766
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1722    2261    1722 
   Size:        539    4227
 
XmaI C'CCGG_G

Cuts at:   1668    1786    1668 
   Size:        118    4648
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AleI
     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbeI     BbsI     BcgI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I     BmrI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI
   BstEII    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI     DraI
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRI     FalI     FspI    FspAI    HaeII    Hin4I   HincII
     KasI     KpnI     MluI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NspI     PciI    PflMI     PmeI
     PpiI    PpuMI    PshAI   PspOMI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI     SfcI
     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AfeI    AflII     AhdI     AscI    AvrII     BaeI    BbvCI
    BfrBI    BmgBI     BmtI    BsiWI    BsmBI    BsrGI   BssHII  BstZ17I
     ClaI    CspCI   DraIII    EcoRV     FseI  HindIII     HpaI     MfeI
     NheI     NotI     NruI     NsiI     PacI     PasI     PfoI     PmlI
     PsiI    PspXI     PsrI    SexAI     SfiI    SgrAI    SnaBI     SrfI
     SspI     SwaI     XhoI     XmnI     ZraI
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