Restriction map for pF1KB7855
 (Linear) (Six-base) MAPSORT of: KIBB7855.seq  Check: 9373  from: 1  to: 1653



KIBB7855.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:50  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1278    1653 
   Size:       1278     375
 
AccI GT'mk_AC

Cuts at:      0    1406    1653 
   Size:       1406     247
 
AleI CACnn'nnGTG

Cuts at:      0    1096    1653 
   Size:       1096     557
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1012    1653 
   Size:       1012     641
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      27    1653 
   Size:         27    1626
 
AlwNI CAG_nnn'CTG

Cuts at:      0     345     864    1018    1177    1225    1428    1431    1543 
   Size:        345     519     154     159      48     203       3     112

Cuts at:   1543    1653 
   Size:        110

  Fragments arranged by size: 

                 519     345     203     159     154     112     110      48
                   3
 
ApaI G_GGCC'C

Cuts at:      0    1521    1560    1653 
   Size:       1521      39      93

  Fragments arranged by size: 

                1521      93      39
 
ApaLI G'TGCA_C

Cuts at:      0     730    1653 
   Size:        730     923
 
ApoI r'AATT_y

Cuts at:      0     853    1653 
   Size:        853     800
 
AvaI C'yCGr_G

Cuts at:      0     704    1560    1653 
   Size:        704     856      93

  Fragments arranged by size: 

                 856     704      93
 
BanII G_rGCy'C

Cuts at:      0     311     581     839     848    1472    1521    1560    1568 
   Size:        311     270     258       9     624      49      39       8

Cuts at:   1568    1653 
   Size:         85

  Fragments arranged by size: 

                 624     311     270     258      85      49      39       9
                   8
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1349    1601    1653 
   Size:       1349     252      52

  Fragments arranged by size: 

                1349     252      52
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     445     479     600     634    1653 
   Size:        445      34     121      34    1019

  Fragments arranged by size: 

                1019     445     121      34      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     290    1653 
   Size:        290    1363
 
BglII A'GATC_T

Cuts at:      0     584     632    1653 
   Size:        584      48    1021

  Fragments arranged by size: 

                1021     584      48
 
BlpI GC'TnA_GC

Cuts at:      0     305    1011    1653 
   Size:        305     706     642

  Fragments arranged by size: 

                 706     642     305
 
Bme1580I G_kGCm'C

Cuts at:      0     564     734    1521    1560    1653 
   Size:        564     170     787      39      93

  Fragments arranged by size: 

                 787     564     170      93      39
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1225    1561    1653 
   Size:       1225     336      92

  Fragments arranged by size: 

                1225     336      92
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     138    1653 
   Size:        138    1515
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     397    1154    1172    1653 
   Size:        397     757      18     481

  Fragments arranged by size: 

                 757     481     397      18
 
Bpu10I CC'TnA_GC

Cuts at:      0    1063    1653 
   Size:       1063     590
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     565    1653 
   Size:        565    1088
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     913    1653 
   Size:        913     740
 
BsaWI w'CCGG_w

Cuts at:      0     755     887    1653 
   Size:        755     132     766

  Fragments arranged by size: 

                 766     755     132
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1588    1618    1653 
   Size:       1588      30      35

  Fragments arranged by size: 

                1588      35      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     162     233     910    1241    1593    1653 
   Size:        162      71     677     331     352      60

  Fragments arranged by size: 

                 677     352     331     162      71      60
 
BseYI C'CCAG_C

Cuts at:      0     695     778    1037    1218    1291    1377    1536    1653 
   Size:        695      83     259     181      73      86     159     117

  Fragments arranged by size: 

                 695     259     181     159     117      86      83      73
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     676    1653 
   Size:        676     977
 
BsiEI CG_ry'CG

Cuts at:      0     831     882    1653 
   Size:        831      51     771

  Fragments arranged by size: 

                 831     771      51
 
BsiHKAI G_wGCw'C

Cuts at:      0     154     311     581     734     839    1069    1653 
   Size:        154     157     270     153     105     230     584

  Fragments arranged by size: 

                 584     270     230     157     154     153     105
 
BsmI GAATG_Cn'

Cuts at:      0     468    1653 
   Size:        468    1185
 
Bsp1286I G_dGCh'C

Cuts at:      0     154     311     564     581     734     839     848    1069 
   Size:        154     157     253      17     153     105       9     221

Cuts at:   1069    1472    1521    1560    1568    1653 
   Size:        403      49      39       8      85

  Fragments arranged by size: 

                 403     253     221     157     154     153     105      85
                  49      39      17       9       8
 
BspEI T'CCGG_A

Cuts at:      0     755    1653 
   Size:        755     898
 
BspHI T'CATG_A

Cuts at:      0     935    1653 
   Size:        935     718
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     872    1278    1653 
   Size:        872     406     375

  Fragments arranged by size: 

                 872     406     375
 
BsrDI GCAATG_nn'

Cuts at:      0     989    1607    1653 
   Size:        989     618      46

  Fragments arranged by size: 

                 989     618      46
 
BsrFI r'CCGG_y

Cuts at:      0     248    1653 
   Size:        248    1405
 
BssSI C'ACGA_G

Cuts at:      0     262    1653 
   Size:        262    1391
 
BstEII G'GTnAC_C

Cuts at:      0     228    1653 
   Size:        228    1425
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      49    1479    1653 
   Size:         49    1430     174

  Fragments arranged by size: 

                1430     174      49
 
BstYI r'GATC_y

Cuts at:      0     584     632    1653 
   Size:        584      48    1021

  Fragments arranged by size: 

                1021     584      48
 
Bsu36I CC'TnA_GG

Cuts at:      0    1253    1490    1653 
   Size:       1253     237     163

  Fragments arranged by size: 

                1253     237     163
 
BtgI C'CryG_G

Cuts at:      0     125     848    1094    1193    1653 
   Size:        125     723     246      99     460

  Fragments arranged by size: 

                 723     460     246     125      99
 
BtsI GCAGTG_nn'

Cuts at:      0     569    1653 
   Size:        569    1084
 
EaeI y'GGCC_r

Cuts at:      0     743    1653 
   Size:        743     910
 
EarI CTCTTCn'nnn_

Cuts at:      0     431     937    1653 
   Size:        431     506     716

  Fragments arranged by size: 

                 716     506     431
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     508    1653 
   Size:        508    1145
 
EcoICRI GAG'CTC

Cuts at:      0     309     579     837    1653 
   Size:        309     270     258     816

  Fragments arranged by size: 

                 816     309     270     258
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     397    1154    1172    1653 
   Size:        397     757      18     481

  Fragments arranged by size: 

                 757     481     397      18
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     379    1162    1653 
   Size:        379     783     491

  Fragments arranged by size: 

                 783     491     379
 
EcoO109I rG'GnC_Cy

Cuts at:      0     237     372     498     512    1215    1246    1314    1352 
   Size:        237     135     126      14     703      31      68      38

Cuts at:   1352    1517    1526    1556    1557    1653 
   Size:        165       9      30       1      96

  Fragments arranged by size: 

                 703     237     165     135     126      96      68      38
                  31      30      14       9       1
 
EcoRI G'AATT_C

Cuts at:      0     853    1653 
   Size:        853     800
 
FspI TGC'GCA

Cuts at:      0     217    1653 
   Size:        217    1436
 
FspAI rTGC'GCAy

Cuts at:      0     217    1653 
   Size:        217    1436
 
HaeII r_GCGC'y

Cuts at:      0     123    1653 
   Size:        123    1530
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1384    1416    1588    1620    1653 
   Size:       1384      32     172      32      33

  Fragments arranged by size: 

                1384     172      33      32      32
 
HincII GTy'rAC

Cuts at:      0    1407    1653 
   Size:       1407     246
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     851    1653 
   Size:        851     802
 
MscI TGG'CCA

Cuts at:      0     745    1653 
   Size:        745     908
 
MslI CAynn'nnrTG

Cuts at:      0     673     994    1096    1447    1477    1653 
   Size:        673     321     102     351      30     176

  Fragments arranged by size: 

                 673     351     321     176     102      30
 
MspA1I CmG'CkG

Cuts at:      0      87     127     323     614     813     956    1326    1381 
   Size:         87      40     196     291     199     143     370      55

Cuts at:   1381    1428    1653 
   Size:         47     225

  Fragments arranged by size: 

                 370     291     225     199     196     143      87      55
                  47      40
 
NcoI C'CATG_G

Cuts at:      0     848    1094    1193    1653 
   Size:        848     246      99     460

  Fragments arranged by size: 

                 848     460     246      99
 
NdeI CA'TA_TG

Cuts at:      0     915    1653 
   Size:        915     738
 
NspI r_CATG'y

Cuts at:      0      96    1653 
   Size:         96    1557
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     345    1653 
   Size:        345    1308
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     589     621    1653 
   Size:        589      32    1032

  Fragments arranged by size: 

                1032     589      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     237     372     498    1653 
   Size:        237     135     126    1155

  Fragments arranged by size: 

                1155     237     135     126
 
PshAI GACnn'nnGTC

Cuts at:      0     881    1653 
   Size:        881     772
 
PspOMI G'GGCC_C

Cuts at:      0    1517    1556    1653 
   Size:       1517      39      97

  Fragments arranged by size: 

                1517      97      39
 
PstI C_TGCA'G

Cuts at:      0     788    1325    1331    1653 
   Size:        788     537       6     322

  Fragments arranged by size: 

                 788     537     322       6
 
PvuI CG_AT'CG

Cuts at:      0     882    1653 
   Size:        882     771
 
PvuII CAG'CTG

Cuts at:      0     614     813    1326    1381    1428    1653 
   Size:        614     199     513      55      47     225

  Fragments arranged by size: 

                 614     513     225     199      55      47
 
RsrII CG'GwC_CG

Cuts at:      0     318    1653 
   Size:        318    1335
 
SacI G_AGCT'C

Cuts at:      0     311     581     839    1653 
   Size:        311     270     258     814

  Fragments arranged by size: 

                 814     311     270     258
 
SacII CC_GC'GG

Cuts at:      0     128    1653 
   Size:        128    1525
 
SalI G'TCGA_C

Cuts at:      0    1405    1653 
   Size:       1405     248
 
SanDI GG'GwC_CC

Cuts at:      0     498    1653 
   Size:        498    1155
 
SapI GCTCTTCn'nnn_

Cuts at:      0     431    1653 
   Size:        431    1222
 
SbfI CC_TGCA'GG

Cuts at:      0     788    1653 
   Size:        788     865
 
SfcI C'TryA_G

Cuts at:      0     431     784    1321    1327    1653 
   Size:        431     353     537       6     326

  Fragments arranged by size: 

                 537     431     353     326       6
 
SmaI CCC'GGG

Cuts at:      0    1562    1653 
   Size:       1562      91
 
SmlI C'TyrA_G

Cuts at:      0     580    1653 
   Size:        580    1073
 
SphI G_CATG'C

Cuts at:      0      96    1653 
   Size:         96    1557
 
StuI AGG'CCT

Cuts at:      0      46    1126    1653 
   Size:         46    1080     527

  Fragments arranged by size: 

                1080     527      46
 
StyI C'CwwG_G

Cuts at:      0     233     848    1094    1133    1193    1653 
   Size:        233     615     246      39      60     460

  Fragments arranged by size: 

                 615     460     246     233      60      39
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1300    1653 
   Size:       1300     353
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1614    1653 
   Size:       1614      39
 
XmaI C'CCGG_G

Cuts at:      0    1560    1653 
   Size:       1560      93
 

 Enzymes that do cut:

     AarI     AccI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AvaI    BanII     BbsI     BcgI     BglI    BglII     BlpI
 Bme1580I     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I
    BspEI    BspHI    BspMI    BsrDI    BsrFI    BssSI   BstEII    BstXI
    BstYI   Bsu36I     BtgI     BtsI     EaeI     EarI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I    EcoRI     FspI    FspAI    HaeII    Hin4I
   HincII     MmeI     MscI     MslI   MspA1I     NcoI     NdeI     NspI
    PflMI     PpiI    PpuMI    PshAI   PspOMI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     SfcI
     SmaI     SmlI     SphI     StuI     StyI    TaqII     XcmI     XmaI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII     AgeI
     AhdI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI
     BbeI    BbvCI    BciVI     BclI    BfrBI    BmgBI     BmtI    BsaAI
    BsaBI    BsaHI    BsiWI    BsmBI    BsrBI    BsrGI   BssHII   BstAPI
    BstBI  BstZ17I    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI    EcoRV     FalI     FseI  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     NaeI     NarI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PasI     PciI     PfoI     PmeI     PmlI     PsiI
    PspXI     PsrI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
    SnaBI     SpeI     SrfI     SspI     SwaI     TatI     TstI  Tth111I
     XbaI     XhoI     XmnI     ZraI
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