Restriction map for pF1KB7355
##################################
# NO CUT ENZYMES (  108 enzymes) #
##################################

Acc65I      AccII       AccIII      AciI        AclI        
AflII       AgeI        AhdI        ApaI        AscI        
AseI        AsnI        Asp718I     AspEI       AspI        
AvaI        AvrII       BalI        BamHI       BanI        
BbeI        BbrPI       BbvCI       BcnI        BfrI        
BglI        BlnI        BsaI        BseAI       BsiEI       
BsiWI       BsoBI       BspDI       BspEI       BsrBI       
BsrFI       BssHII      BstAPI      BstBI       BstEII      
BstPI       BstUI       Cfr10I      ClaI        CpoI        
DraIII      DsaI        EaeI        EagI        Eam1105I    
EclXI       Eco52I      EcoNI       EcoO65I     EcoRV       
EcoT22I     FauI        FseI        HindIII     HpaI        
KasI        KpnI        KspI        MfeI        MluI        
MluNI       MroI        MscI        MunI        MvnI        
NaeI        NarI        NciI        NcoI        NgoMIV      
NotI        NruI        NsiI        NspV        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        RsrII       
SacI        SacII       SalI        SbfI        ScaI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        Sse8387I    SwaI        Tth111I     
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AatII        1469  GACGTC               GACGT^C
AccI         1486  GTATAC               GT^[AC][GT]AC
Alw44I       1335  GTGCAC               G^TGCAC
AocI          949  CCTCAGG              CC^T[ACGT]AGG
Aor51HI      2007  AGCGCT               AGC^GCT
ApaLI        1335  GTGCAC               G^TGCAC
Asp700I      3039  GAAGTATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII        3218  TGCGCA               TGC^GCA
BanII        3019  GGGCTC               G[GA]GC[CT]^C
BcgI         1392  CGACCAAAATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI         1686  TGATCA               T^GATCA
BlpI          175  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      175  GCTCAGC              GC^T[ACGT]AGC
BsaAI         274  TACGTG               [CT]AC^GT[GA]
BsaBI         246  GATGGACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI        2787  CTCCTC               GAGGAG(10/8)
BsmBI        1765  GAGACG               CGTCTC(1/5)
BsmI         2259  GAATGC               GAATGC(1/-1)
Bsp1407I     2293  TGTACA               T^GTACA
BspMI         648  GCAGGT               ACCTGC(4/8)
BsrDI         330  CATTGC               GCAATG(2/0)
BsrGI        2293  TGTACA               T^GTACA
BssSI        1926  CACGAG               CACGAG(-5/-1)
Bst1107I     1486  GTATAC               GTA^TAC
BstXI         742  CCAACAACTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I      1486  GTATAC               GTA^TAC
Bsu36I        949  CCTCAGG              CC^T[ACGT]AGG
CelII         175  GCTCAGC              GC^T[ACGT]AGC
Eco47III     2007  AGCGCT               AGC^GCT
Eco81I        949  CCTCAGG              CC^T[ACGT]AGG
EcoRI        2378  GAATTC               G^AATTC
FbaI         1686  TGATCA               T^GATCA
FspI         3218  TGCGCA               TGC^GCA
HgaI         2546  GCGTC                GACGC(5/10)
HincII        140  GTCAAC               GT[CT]^[GA]AC
HindII        140  GTCAAC               GT[CT]^[GA]AC
MamI          246  GATGGACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NheI          570  GCTAGC               G^CTAGC
PacI          916  TTAATTAA             TTAAT^TAA
SapI         1372  GCTCTTC              GCTCTTC(1/4)
SexAI         485  ACCTGGT              A^CC[AT]GGT
SphI          574  GCATGC               GCATG^C
SspBI        2293  TGTACA               T^GTACA
SspI         2106  AATATT               AAT^ATT
XcmI         3005  CCAGGCCTCTCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI         3039  GAAGTATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AcyI         1469  GACGTC               G[GA]^CG[CT]C
             2545  GGCGTC              
AspHI        1335  GTGCAC               G[AT]GC[AT]^C
             1370  GTGCTC              
BglII        2031  AGATCT               A^GATCT
             2166  AGATCT              
BsaHI        1469  GACGTC               G[GA]^CG[CT]C
             2545  GGCGTC              
BsaWI        1603  ACCGGA               [AT]^CCGG[AT]
             2642  ACCGGA              
BsiHKAI      1335  GTGCAC               G[AT]GC[AT]^C
             1370  GTGCTC              
DraII         871  AGGTCCT              [GA]G^G[ACGT]CC[CT]
             3098  AGGTCCT             
EcoO109I      871  AGGTCCT              [GA]G^G[ACGT]CC[CT]
             3098  AGGTCCT             
EcoT14I       906  CCTTGG               C^C[AT][AT]GG
             2439  CCTTGG              
HaeII           3  GGCGCT               [GA]GCGC^[CT]
             2007  AGCGCT              
HaeIII       1230  GGCC                 GG^CC
             3008  GGCC                
HapII        1604  CCGG                 C^CGG
             2643  CCGG                
Hin1I        1469  GACGTC               G[GA]^CG[CT]C
             2545  GGCGTC              
HpaII        1604  CCGG                 C^CGG
             2643  CCGG                
MspA1I       1021  CAGCTG               C[AC]G^C[GT]G
             2793  CAGCTG              
MspI         1604  CCGG                 C^CGG
             2643  CCGG                
NdeI         1676  CATATG               CA^TATG
             2695  CATATG              
PflMI          15  CCAGAGTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1429  CCATATTCTGG         
PpuMI         871  AGGTCCT              [GA]G^G[AT]CC[CT]
             3098  AGGTCCT             
PstI          713  CTGCAG               CTGCA^G
             2935  CTGCAG              
PvuII        1021  CAGCTG               CAG^CTG
             2793  CAGCTG              
SmlI         2479  CTCAAG               C^T[CT][GA]AG
             2509  CTCAAG              
StuI         1229  AGGCCT               AGG^CCT
             3007  AGGCCT              
StyI          906  CCTTGG               C^C[AT][AT]GG
             2439  CCTTGG              
TfiI          709  GAATC                G^A[AT]TC
              738  GATTC               
Van91I         15  CCAGAGTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1429  CCATATTCTGG         
XbaI          499  TCTAGA               T^CTAGA
             1761  TCTAGA              


#########################################
# 3 OR MORE CUT ENZYMES (   77 enzymes) #
#########################################

AcsI        AfaI        AflIII      AluI        AlwI        
AlwNI       ApoI        AvaII       BbsI        BbvI        
BciVI       BfaI        BmyI        BpmI        BpuAI       
BsaJI       BsgI        BsiYI       BslI        BsmAI       
BsmFI       Bsp1286I    BspHI       BspLU11I    BsrI        
BssKI       BstF5I      BstNI       BstYI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraI        
DrdI        EarI        EcoRII      Fnu4HI      FokI        
HhaI        HinP1I      HinfI       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MnlI        MseI        MslI        
MvaI        MwoI        NdeII       NlaIII      NlaIV       
NspI        PleI        RcaI        RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        TaqI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     XhoII       
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