Restriction map for pF1KB7355
 (Linear) (Six-base) MAPSORT of: KIBB7355.seq  Check: 4687  from: 1  to: 3264



KIBB7355.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:08  ..

AatII G_ACGT'C

Cuts at:      0    1473    3264 
   Size:       1473    1791
 
AccI GT'mk_AC

Cuts at:      0    1487    3264 
   Size:       1487    1777
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     369     491     601    1076    1822    1965    2239    2373 
   Size:        369     122     110     475     746     143     274     134

Cuts at:   2373    3099    3264 
   Size:        726     165

  Fragments arranged by size: 

                 746     726     475     369     274     165     143     134
                 122     110
 
AfeI AGC'GCT

Cuts at:      0    2009    3264 
   Size:       2009    1255
 
AflIII A'CryG_T

Cuts at:      0     193    1366    2290    3264 
   Size:        193    1173     924     974

  Fragments arranged by size: 

                1173     974     924     193
 
AleI CACnn'nnGTG

Cuts at:      0    1928    3264 
   Size:       1928    1336
 
AlwNI CAG_nnn'CTG

Cuts at:      0    2433    2798    3147    3264 
   Size:       2433     365     349     117

  Fragments arranged by size: 

                2433     365     349     117
 
ApaLI G'TGCA_C

Cuts at:      0    1335    3264 
   Size:       1335    1929
 
ApoI r'AATT_y

Cuts at:      0     982    1108    1906    2053    2061    2352    2378    3264 
   Size:        982     126     798     147       8     291      26     886

  Fragments arranged by size: 

                 982     886     798     291     147     126      26       8
            
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    2768    2801    3264 
   Size:       2768      33     463

  Fragments arranged by size: 

                2768     463      33
 
BanII G_rGCy'C

Cuts at:      0    3023    3264 
   Size:       3023     241
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     375    2541    2984    3264 
   Size:        375    2166     443     280

  Fragments arranged by size: 

                2166     443     375     280
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1381    1415    3264 
   Size:       1381      34    1849

  Fragments arranged by size: 

                1849    1381      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     929    1593    3012    3264 
   Size:        929     664    1419     252

  Fragments arranged by size: 

                1419     929     664     252
 
BclI T'GATC_A

Cuts at:      0    1686    3264 
   Size:       1686    1578
 
BglII A'GATC_T

Cuts at:      0    2031    2166    3264 
   Size:       2031     135    1098

  Fragments arranged by size: 

                2031    1098     135
 
BlpI GC'TnA_GC

Cuts at:      0     176    3264 
   Size:        176    3088
 
Bme1580I G_kGCm'C

Cuts at:      0    1339    3264 
   Size:       1339    1925
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1113    1812    3102    3264 
   Size:       1113     699    1290     162

  Fragments arranged by size: 

                1290    1113     699     162
 
BmtI G_CTAG'C

Cuts at:      0     574    3264 
   Size:        574    2690
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     403    2572    2775    3264 
   Size:        403    2169     203     489

  Fragments arranged by size: 

                2169     489     403     203
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2464    2494    3264 
   Size:       2464      30     770

  Fragments arranged by size: 

                2464     770      30
 
BsaAI yAC'GTr

Cuts at:      0     276    3264 
   Size:        276    2988
 
BsaBI GATnn'nnATC

Cuts at:      0     250    3264 
   Size:        250    3014
 
BsaHI Gr'CG_yC

Cuts at:      0    1470    2546    3264 
   Size:       1470    1076     718

  Fragments arranged by size: 

                1470    1076     718
 
BsaWI w'CCGG_w

Cuts at:      0    1603    2642    3264 
   Size:       1603    1039     622

  Fragments arranged by size: 

                1603    1039     622
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1019    1049    1318    1348    3264 
   Size:       1019      30     269      30    1916

  Fragments arranged by size: 

                1916    1019     269      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    2778    3264 
   Size:       2778     486
 
BseYI C'CCAG_C

Cuts at:      0    1023    2342    3264 
   Size:       1023    1319     922

  Fragments arranged by size: 

                1319    1023     922
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     754    1402    3264 
   Size:        754     648    1862

  Fragments arranged by size: 

                1862     754     648
 
BsiHKAI G_wGCw'C

Cuts at:      0    1339    1374    3264 
   Size:       1339      35    1890

  Fragments arranged by size: 

                1890    1339      35
 
BsmI GAATG_Cn'

Cuts at:      0    2265    3264 
   Size:       2265     999
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1759    3264 
   Size:       1759    1505
 
Bsp1286I G_dGCh'C

Cuts at:      0    1339    1374    3023    3264 
   Size:       1339      35    1649     241

  Fragments arranged by size: 

                1649    1339     241      35
 
BspHI T'CATG_A

Cuts at:      0     978    1772    2957    3264 
   Size:        978     794    1185     307

  Fragments arranged by size: 

                1185     978     794     307
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     639    3264 
   Size:        639    2625
 
BsrDI GCAATG_nn'

Cuts at:      0     329    3264 
   Size:        329    2935
 
BsrGI T'GTAC_A

Cuts at:      0    2293    3264 
   Size:       2293     971
 
BssSI C'ACGA_G

Cuts at:      0    1926    3264 
   Size:       1926    1338
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     749    3264 
   Size:        749    2515
 
BstYI r'GATC_y

Cuts at:      0     783    2031    2166    2338    2429    3264 
   Size:        783    1248     135     172      91     835

  Fragments arranged by size: 

                1248     835     783     172     135      91
 
BstZ17I GTA'TAC

Cuts at:      0    1488    3264 
   Size:       1488    1776
 
Bsu36I CC'TnA_GG

Cuts at:      0     950    3264 
   Size:        950    2314
 
BtsI GCAGTG_nn'

Cuts at:      0     435    3228    3264 
   Size:        435    2793      36

  Fragments arranged by size: 

                2793     435      36
 
DraI TTT'AAA

Cuts at:      0     587     611    1007    1877    2124    3264 
   Size:        587      24     396     870     247    1140

  Fragments arranged by size: 

                1140     870     587     396     247      24
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     379     870    2818    3264 
   Size:        379     491    1948     446

  Fragments arranged by size: 

                1948     491     446     379
 
EarI CTCTTCn'nnn_

Cuts at:      0    1379    1841    2392    2836    3192    3264 
   Size:       1379     462     551     444     356      72

  Fragments arranged by size: 

                1379     551     462     444     356      72
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     369     403     491     601    1076    1822    1965    2239 
   Size:        369      34      88     110     475     746     143     274

Cuts at:   2239    2373    2572    2775    3099    3264 
   Size:        134     199     203     324     165

  Fragments arranged by size: 

                 746     475     369     324     274     203     199     165
                 143     134     110      88      34
 
EcoO109I rG'GnC_Cy

Cuts at:      0     872    3099    3264 
   Size:        872    2227     165

  Fragments arranged by size: 

                2227     872     165
 
EcoRI G'AATT_C

Cuts at:      0    2378    3264 
   Size:       2378     886
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1489    2535    3264 
   Size:       1489    1046     729

  Fragments arranged by size: 

                1489    1046     729
 
FspI TGC'GCA

Cuts at:      0    3220    3264 
   Size:       3220      44
 
HaeII r_GCGC'y

Cuts at:      0       7    2011    3264 
   Size:          7    2004    1253

  Fragments arranged by size: 

                2004    1253       7
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     440     472    1002    1034    1356    1388    2016    2048 
   Size:        440      32     530      32     322      32     628      32

Cuts at:   2048    2350    2382    3264 
   Size:        302      32     882

  Fragments arranged by size: 

                 882     628     530     440     322     302      32      32
                  32      32      32
 
HincII GTy'rAC

Cuts at:      0     142    3264 
   Size:        142    3122
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     766    1229    1304    2965    3264 
   Size:        766     463      75    1661     299

  Fragments arranged by size: 

                1661     766     463     299      75
 
MslI CAynn'nnrTG

Cuts at:      0     840    1267    1283    1928    2868    3264 
   Size:        840     427      16     645     940     396

  Fragments arranged by size: 

                 940     840     645     427     396      16
 
MspA1I CmG'CkG

Cuts at:      0    1023    2795    3264 
   Size:       1023    1772     469

  Fragments arranged by size: 

                1772    1023     469
 
NdeI CA'TA_TG

Cuts at:      0    1677    2696    3264 
   Size:       1677    1019     568

  Fragments arranged by size: 

                1677    1019     568
 
NheI G'CTAG_C

Cuts at:      0     570    3264 
   Size:        570    2694
 
NspI r_CATG'y

Cuts at:      0     197     578    1370    2294    3264 
   Size:        197     381     792     924     970

  Fragments arranged by size: 

                 970     924     792     381     197
 
PacI TTA_AT'TAA

Cuts at:      0     920    3264 
   Size:        920    2344
 
PciI A'CATG_T

Cuts at:      0     193    1366    2290    3264 
   Size:        193    1173     924     974

  Fragments arranged by size: 

                1173     974     924     193
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      21    1435    3264 
   Size:         21    1414    1829

  Fragments arranged by size: 

                1829    1414      21
 
PfoI T'CCnGG_A

Cuts at:      0     764    2672    3264 
   Size:        764    1908     592

  Fragments arranged by size: 

                1908     764     592
 
PpuMI rG'GwC_Cy

Cuts at:      0     872    3099    3264 
   Size:        872    2227     165

  Fragments arranged by size: 

                2227     872     165
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    1151    1183    3264 
   Size:       1151      32    2081

  Fragments arranged by size: 

                2081    1151      32
 
PstI C_TGCA'G

Cuts at:      0     717    2939    3264 
   Size:        717    2222     325

  Fragments arranged by size: 

                2222     717     325
 
PvuII CAG'CTG

Cuts at:      0    1023    2795    3264 
   Size:       1023    1772     469

  Fragments arranged by size: 

                1772    1023     469
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1379    3264 
   Size:       1379    1885
 
SexAI A'CCwGG_T

Cuts at:      0     485    3264 
   Size:        485    2779
 
SfcI C'TryA_G

Cuts at:      0     713    1096    2935    3264 
   Size:        713     383    1839     329

  Fragments arranged by size: 

                1839     713     383     329
 
SmlI C'TyrA_G

Cuts at:      0    2479    2509    3264 
   Size:       2479      30     755

  Fragments arranged by size: 

                2479     755      30
 
SphI G_CATG'C

Cuts at:      0     578    3264 
   Size:        578    2686
 
SspI AAT'ATT

Cuts at:      0    2108    3264 
   Size:       2108    1156
 
StuI AGG'CCT

Cuts at:      0    1231    3009    3264 
   Size:       1231    1778     255

  Fragments arranged by size: 

                1778    1231     255
 
StyI C'CwwG_G

Cuts at:      0     906    2439    3264 
   Size:        906    1533     825

  Fragments arranged by size: 

                1533     906     825
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    2026    3190    3264 
   Size:       2026    1164      74

  Fragments arranged by size: 

                2026    1164      74
 
TatI w'GTAC_w

Cuts at:      0      43    1531    2293    2583    2727    3264 
   Size:         43    1488     762     290     144     537

  Fragments arranged by size: 

                1488     762     537     290     144      43
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1318    1350    3264 
   Size:       1318      32    1914

  Fragments arranged by size: 

                1914    1318      32
 
XbaI T'CTAG_A

Cuts at:      0     499    1761    3264 
   Size:        499    1262    1503

  Fragments arranged by size: 

                1503    1262     499
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    3012    3264 
   Size:       3012     252
 
XmnI GAAnn'nnTTC

Cuts at:      0    3043    3264 
   Size:       3043     221
 
ZraI GAC'GTC

Cuts at:      0    1471    3264 
   Size:       1471    1793
 

 Enzymes that do cut:

    AatII     AccI     AcuI     AfeI   AflIII     AleI    AlwNI    ApaLI
     ApoI     BaeI    BanII     BbsI     BcgI    BciVI     BclI    BglII
     BlpI Bme1580I     BmrI     BmtI     BpmI    BpuEI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI     BsmI
    BsmBI Bsp1286I    BspHI    BspMI    BsrDI    BsrGI    BssSI    BstXI
    BstYI  BstZ17I   Bsu36I     BtsI     DraI     DrdI     EarI  Eco57MI
 EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I   HincII     MmeI
     MslI   MspA1I     NdeI     NheI     NspI     PacI     PciI    PflMI
     PfoI    PpuMI     PsrI     PstI    PvuII     SapI    SexAI     SfcI
     SmlI     SphI     SspI     StuI     StyI    TaqII     TatI     TstI
     XbaI     XcmI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI   Acc65I     AclI    AflII     AgeI     AhdI     AlfI     AloI
     ApaI     AscI     AseI    AsiSI     AvaI    AvrII    BamHI     BanI
     BbeI    BbvCI    BfrBI     BglI    BmgBI     BplI   Bpu10I     BsaI
    BsiEI    BsiWI    BspEI    BsrBI    BsrFI   BssHII   BstAPI    BstBI
   BstEII     BtgI    BtgZI     ClaI    CspCI   DraIII     EaeI     EagI
     EciI  EcoICRI    EcoNI    EcoRV     FseI    FspAI  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MscI     NaeI     NarI     NcoI
   NgoMIV     NotI     NruI     NsiI     PasI     PmeI     PmlI     PpiI
    PshAI     PsiI   PspOMI    PspXI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SrfI     SwaI  Tth111I     XhoI     XmaI
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