Restriction map for pF1KB6485
##################################
# NO CUT ENZYMES (  123 enzymes) #
##################################

AatII       Acc65I      AccII       AclI        AcsI        
AcyI        AfaI        AflII       AflIII      AgeI        
AhdI        Alw44I      Aor51HI     ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AviII       AvrII       BanI        
BbeI        BbrPI       BbvCI       BcgI        BciVI       
BfaI        BfrI        BglI        BglII       BlnI        
BlpI        Bpu1102I    BsaAI       BsaBI       BsaHI       
BsgI        BsiWI       BsmBI       BsmI        Bsp1407I    
BspDI       BspHI       BspLU11I    BsrBI       BsrDI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstUI       BstXI       BstZ17I     CelII       
ClaI        CpoI        DraI        DraIII      DrdI        
Eam1105I    Eco47III    EcoRI       EcoRV       FseI        
FspI        Hin1I       HincII      HindII      HindIII     
HpaI        KasI        KpnI        KspI        MaeI        
MamI        MfeI        MluI        MseI        MunI        
MvnI        NarI        NdeI        NheI        NotI        
NruI        NspV        PacI        PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsaI        RsrII       SacII       
SalI        SapI        SbfI        ScaI        SfiI        
SfoI        SfuI        SgrAI       SnaBI       SpeI        
Sse8387I    SspBI       SspI        StuI        SwaI        
Tru9I       XbaI        XmnI        


###################################
# ONE CUT ENZYMES (   56 enzymes) #
###################################

AccI          925  GTAGAC               GT^[AC][GT]AC
AccIII        491  TCCGGA               T^CCGGA
AocI          156  CCTGAGG              CC^T[ACGT]AGG
AspI          616  GACTCGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI          884  TGGCCA               TGG^CCA
BamHI         497  GGATCC               G^GATCC
BanII         550  GAGCTC               G[GA]GC[CT]^C
BclI          975  TGATCA               T^GATCA
BsaI          286  GGTCTC               GGTCTC(1/5)
BsaWI         491  TCCGGA               [AT]^CCGG[AT]
BseAI         491  TCCGGA               T^CCGGA
BsiEI         864  CGGCCG               CG[GA][CT]^CG
BspEI         491  TCCGGA               T^CCGGA
BsrFI         866  GCCGGC               [GA]^CCGG[CT]
BstEII        738  GGTGACC              G^GT[ACGT]ACC
BstPI         738  GGTGACC              G^GT[ACGT]ACC
BstYI         497  GGATCC               [GA]^GATC[CT]
Bsu36I        156  CCTGAGG              CC^T[ACGT]AGG
Cfr10I        866  GCCGGC               [GA]^CCGG[CT]
DsaI          375  CCATGG               C^C[GA][CT]GG
EagI          864  CGGCCG               C^GGCCG
EarI          109  GAAGAG               CTCTTC(1/4)
EclXI         864  CGGCCG               C^GGCCG
Eco52I        864  CGGCCG               C^GGCCG
Eco81I        156  CCTGAGG              CC^T[ACGT]AGG
EcoO65I       738  GGTGACC              G^GT[ACGT]ACC
EcoT22I       512  ATGCAT               ATGCA^T
FbaI          975  TGATCA               T^GATCA
HaeII         202  GGCGCT               [GA]GCGC^[CT]
HgaI          794  GACGC                GACGC(5/10)
Ksp632I       109  GAAGAG               CTCTTC(1/4)
MaeII         392  ACGT                 A^CGT
MflI          497  GGATCC               [GA]^GATC[CT]
MluNI         884  TGGCCA               TGG^CCA
MroI          491  TCCGGA               T^CCGGA
MscI          884  TGGCCA               TGG^CCA
NaeI          866  GCCGGC               GCC^GGC
NcoI          375  CCATGG               C^CATGG
NgoMIV        866  GCCGGC               G^CCGGC
NsiI          512  ATGCAT               ATGCA^T
NspI          510  GCATGC               [GA]CATG^[CT]
PaeR7I        755  CTCGAG               C^TCGAG
PflFI         616  GACTCGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PleI          616  GACTC                GAGTC(4/5)
SacI          550  GAGCTC               GAGCT^C
SexAI          89  ACCTGGT              A^CC[AT]GGT
SmaI          713  CCCGGG               CCC^GGG
SmlI          755  CTCGAG               C^T[CT][GA]AG
SphI          510  GCATGC               GCATG^C
Tsp509I       312  AATT                 ^AATT
Tth111I       616  GACTCGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI          570  CCAGCAGGTGACTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI          755  CTCGAG               C^TCGAG
XhoII         497  GGATCC               [GA]^GATC[CT]
XmaCI         713  CCCGGG               C^CCGGG
XmaI          713  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AlwNI         529  CAGATGCTG            CAG[ACGT][ACGT][ACGT]^CTG
              590  CAGGAACTG           
AspHI           7  GTGCTC               G[AT]GC[AT]^C
              550  GAGCTC              
BbsI          225  GAAGAC               GAAGAC(2/6)
              702  GAAGAC              
BcnI          713  CCCGG                CC^[GC]GG
              714  CCGGG               
BpuAI         225  GAAGAC               GAAGAC(2/6)
              702  GAAGAC              
BsiHKAI         7  GTGCTC               G[AT]GC[AT]^C
              550  GAGCTC              
BspMI         783  ACCTGC               ACCTGC(4/8)
              573  GCAGGT              
CfoI          203  GCGC                 GCG^C
              870  GCGC                
EaeI          864  CGGCCG               [CT]^GGCC[GA]
              884  TGGCCA              
EcoNI          33  CCTTACCGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              105  CCTGGAAGAGG         
EcoT14I       375  CCATGG               C^C[AT][AT]GG
              720  CCTTGG              
HhaI          203  GCGC                 GCG^C
              870  GCGC                
HinP1I        203  GCGC                 G^CGC
              870  GCGC                
MslI          624  CACTGCGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              726  CATGGCAGTG          
NciI          713  CCCGG                CC^[GC]GG
              714  CCGGG               
PflMI         528  CCAGATGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              915  CCAGGAGATGG         
PvuII         442  CAGCTG               CAG^CTG
              685  CAGCTG              
StyI          375  CCATGG               C^C[AT][AT]GG
              720  CCTTGG              
TaqI          756  TCGA                 T^CGA
              842  TCGA                
TfiI          406  GATTC                G^A[AT]TC
              825  GATTC               
TthHB8I       756  TCGA                 T^CGA
              842  TCGA                
Van91I        528  CCAGATGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              915  CCAGGAGATGG         


#########################################
# 3 OR MORE CUT ENZYMES (   57 enzymes) #
#########################################

AciI        AluI        AlwI        AvaI        AvaII       
BbvI        BmyI        BpmI        BsaJI       BseRI       
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BsrI        BssKI       BstF5I      BstNI       
Cac8I       Cfr13I      DdeI        DpnII       DraII       
EcoO109I    EcoRII      FauI        Fnu4HI      FokI        
HaeIII      HapII       HinfI       HpaII       HphI        
ItaI        MaeIII      MboI        MboII       MnlI        
MspA1I      MspI        MvaI        MwoI        NdeII       
NlaIII      NlaIV       PpuMI       PstI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        TseI        
Tsp45I      TspRI       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com