Restriction map for pF1KB7253
##################################
# NO CUT ENZYMES (  162 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwNI       AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp718I     AspEI       AspHI       AspI        AvaI        
AviII       AvrII       BamHI       BanI        BanII       
BbeI        BbrPI       BbvCI       BcgI        BcnI        
BfrI        BglI        BglII       BlnI        BlpI        
BmyI        Bpu1102I    BsaAI       BsaBI       BsaHI       
BsaI        BsaWI       BseAI       BseRI       BsiEI       
BsiHKAI     BsiWI       BsmAI       BsmBI       BsoBI       
Bsp1286I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrBI       BsrDI       BsrFI       BssHII      
BssSI       Bst1107I    BstBI       BstEII      BstPI       
BstUI       BstXI       BstZ17I     Bsu36I      CelII       
Cfr10I      ClaI        CpoI        DraI        DraIII      
DsaI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO65I     EcoRI       
EcoRV       EcoT14I     EcoT22I     FseI        FspI        
HaeII       HapII       Hin1I       HindIII     HpaI        
HpaII       KasI        KpnI        KspI        MaeIII      
MamI        MfeI        MluI        MroI        MspA1I      
MspI        MunI        MvnI        NaeI        NarI        
NciI        NcoI        NdeI        NgoMIV      NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PinAI       PleI        PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
PvuII       RcaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    StyI        SwaI        
Tsp45I      Tth111I     XbaI        XcmI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   34 enzymes) #
###################################

AlwI         1106  GGATC                GGATC(4/5)
BalI          996  TGGCCA               TGG^CCA
BciVI        1020  GTATCC               GTATCC(6/5)
BclI         1056  TGATCA               T^GATCA
BpmI           17  CTCCAG               CTGGAG(16/14)
BsaJI         276  CCCTGG               C^C[ACGT][ACGT]GG
BstAPI        937  GCAGCAGATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI        1106  GGATCT               [GA]^GATC[CT]
CfoI          117  GCGC                 GCG^C
DraII         135  GGGACCT              [GA]G^G[ACGT]CC[CT]
DrdI         1192  GACAAAGATGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI          996  TGGCCA               [CT]^GGCC[GA]
EarI          977  CTCTTC               CTCTTC(1/4)
EcoO109I      135  GGGACCT              [GA]G^G[ACGT]CC[CT]
FbaI         1056  TGATCA               T^GATCA
HgaI          906  GACGC                GACGC(5/10)
HhaI          117  GCGC                 GCG^C
HinP1I        117  GCGC                 G^CGC
HincII        505  GTCAAC               GT[CT]^[GA]AC
HindII        505  GTCAAC               GT[CT]^[GA]AC
Ksp632I       977  CTCTTC               CTCTTC(1/4)
MaeII         326  ACGT                 A^CGT
MflI         1106  GGATCT               [GA]^GATC[CT]
MluNI         996  TGGCCA               TGG^CCA
MscI          996  TGGCCA               TGG^CCA
MslI           46  CATGGTGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NheI          574  GCTAGC               G^CTAGC
NspI          509  ACATGC               [GA]CATG^[CT]
PpuMI         135  GGGACCT              [GA]G^G[AT]CC[CT]
SexAI          81  ACCAGGT              A^CC[AT]GGT
SmlI          725  CTCAAG               C^T[CT][GA]AG
SspI          808  AATATT               AAT^ATT
StuI         1079  AGGCCT               AGG^CCT
XhoII        1106  GGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AciI            5  CCGC                 CCGC(-3/-1)
               26  CCGC                
AluI           21  AGCT                 AG^CT
             1131  AGCT                
Asp700I       338  GAATGTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              620  GAAATAATTC          
BbsI          342  GTCTTC               GAAGAC(2/6)
             1005  GTCTTC              
BbvI          937  GCAGC                GCAGC(8/12)
              202  GCTGC               
BpuAI         342  GTCTTC               GAAGAC(2/6)
             1005  GTCTTC              
BsgI          948  CTGCAC               GTGCAG(16/14)
              935  GTGCAG              
BsmFI         135  GGGAC                GGGAC(10/14)
              904  GGGAC               
BsmI          314  GAATGC               GAATGC(1/-1)
              570  GAATGC              
Bsp1407I       87  TGTACA               T^GTACA
             1036  TGTACA              
BsrGI          87  TGTACA               T^GTACA
             1036  TGTACA              
DpnII        1057  GATC                 ^GATC
             1107  GATC                
FauI            4  CCCGC                CCCGC(4/6)
               25  CCCGC               
Fnu4HI        202  GCTGC                GC^[ACGT]GC
              937  GCAGC               
ItaI          202  GCTGC                GC^[ACGT]GC
              937  GCAGC               
MboI         1057  GATC                 ^GATC
             1107  GATC                
NdeII        1057  GATC                 ^GATC
             1107  GATC                
NlaIV         135  GGGACC               GG[ACGT]^[ACGT]CC
              374  GGAACC              
Sau3AI       1057  GATC                 ^GATC
             1107  GATC                
SspBI          87  TGTACA               T^GTACA
             1036  TGTACA              
TaqI          423  TCGA                 T^CGA
              831  TCGA                
TseI          202  GCTGC                G^C[AT]GC
              937  GCAGC               
TthHB8I       423  TCGA                 T^CGA
              831  TCGA                
XmnI          338  GAATGTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              620  GAAATAATTC          


#########################################
# 3 OR MORE CUT ENZYMES (   38 enzymes) #
#########################################

AcsI        AfaI        ApoI        AvaII       BfaI        
BsiYI       BslI        BsrI        BssKI       BstF5I      
BstNI       Cac8I       Cfr13I      DdeI        EcoRII      
FokI        HaeIII      HinfI       HphI        MaeI        
MboII       MnlI        MseI        MvaI        MwoI        
NlaIII      PflMI       PstI        RsaI        Sau96I      
ScrFI       SfaNI       SfcI        TfiI        Tru9I       
Tsp509I     TspRI       Van91I      
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