Restriction map for pF1KE0481
 (Linear) (Six-base) MAPSORT of: KIEE0481.seq  Check: 1512  from: 1  to: 1257



KIEE0481.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:15  ..

AccI GT'mk_AC

Cuts at:      0     251     628    1257 
   Size:        251     377     629

  Fragments arranged by size: 

                 629     377     251
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1201    1257 
   Size:       1201      56
 
ApoI r'AATT_y

Cuts at:      0    1007    1257 
   Size:       1007     250
 
AseI AT'TA_AT

Cuts at:      0    1103    1257 
   Size:       1103     154
 
AvaI C'yCGr_G

Cuts at:      0     338    1257 
   Size:        338     919
 
BamHI G'GATC_C

Cuts at:      0     352     878    1257 
   Size:        352     526     379

  Fragments arranged by size: 

                 526     379     352
 
BanII G_rGCy'C

Cuts at:      0      53    1172    1257 
   Size:         53    1119      85

  Fragments arranged by size: 

                1119      85      53
 
BfrBI ATG'CAT

Cuts at:      0     313    1257 
   Size:        313     944
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     658     682     887     961    1257 
   Size:        658      24     205      74     296

  Fragments arranged by size: 

                 658     296     205      74      24
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1063    1257 
   Size:       1063     194
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     438     451     503    1257 
   Size:        438      13      52     754

  Fragments arranged by size: 

                 754     438      52      13
 
Bpu10I CC'TnA_GC

Cuts at:      0     440    1257 
   Size:        440     817
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     472    1257 
   Size:        472     785
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     410     694    1257 
   Size:        410     284     563

  Fragments arranged by size: 

                 563     410     284
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0       9    1257 
   Size:          9    1248
 
Bsp1286I G_dGCh'C

Cuts at:      0      53    1172    1257 
   Size:         53    1119      85

  Fragments arranged by size: 

                1119      85      53
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      95    1257 
   Size:         95    1162
 
BsrFI r'CCGG_y

Cuts at:      0     187    1080    1257 
   Size:        187     893     177

  Fragments arranged by size: 

                 893     187     177
 
BssSI C'ACGA_G

Cuts at:      0     900    1257 
   Size:        900     357
 
BstYI r'GATC_y

Cuts at:      0     352     878    1257 
   Size:        352     526     379

  Fragments arranged by size: 

                 526     379     352
 
BstZ17I GTA'TAC

Cuts at:      0     629    1257 
   Size:        629     628
 
BtgI C'CryG_G

Cuts at:      0     482    1257 
   Size:        482     775
 
BtsI GCAGTG_nn'

Cuts at:      0      95     687     966    1257 
   Size:         95     592     279     291

  Fragments arranged by size: 

                 592     291     279      95
 
DraI TTT'AAA

Cuts at:      0      74    1257 
   Size:         74    1183
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1132    1257 
   Size:       1132     125
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     438     451     503    1257 
   Size:        438      13      52     754

  Fragments arranged by size: 

                 754     438      52      13
 
EcoO109I rG'GnC_Cy

Cuts at:      0     219     599     637     916    1257 
   Size:        219     380      38     279     341

  Fragments arranged by size: 

                 380     341     279     219      38
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     786    1257 
   Size:        786     471
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     337     369     534     566    1118    1150    1257 
   Size:        337      32     165      32     552      32     107

  Fragments arranged by size: 

                 552     337     165     107      32      32      32
 
NcoI C'CATG_G

Cuts at:      0     482    1257 
   Size:        482     775
 
NdeI CA'TA_TG

Cuts at:      0     243    1257 
   Size:        243    1014
 
NsiI A_TGCA'T

Cuts at:      0     315    1257 
   Size:        315     942
 
PasI CC'CwG_GG

Cuts at:      0     473     752     851    1257 
   Size:        473     279      99     406

  Fragments arranged by size: 

                 473     406     279      99
 
PpuMI rG'GwC_Cy

Cuts at:      0     219     599     637     916    1257 
   Size:        219     380      38     279     341

  Fragments arranged by size: 

                 380     341     279     219      38
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     793     825    1257 
   Size:        793      32     432

  Fragments arranged by size: 

                 793     432      32
 
PstI C_TGCA'G

Cuts at:      0     636    1257 
   Size:        636     621
 
SfcI C'TryA_G

Cuts at:      0     632    1257 
   Size:        632     625
 
SmaI CCC'GGG

Cuts at:      0     340    1257 
   Size:        340     917
 
StyI C'CwwG_G

Cuts at:      0     482    1257 
   Size:        482     775
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     604     956    1257 
   Size:        604     352     301

  Fragments arranged by size: 

                 604     352     301
 
Tth111I GACn'n_nGTC

Cuts at:      0     688     967    1257 
   Size:        688     279     290

  Fragments arranged by size: 

                 688     290     279
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     480    1257 
   Size:        480     777
 
XmaI C'CCGG_G

Cuts at:      0     338    1257 
   Size:        338     919
 
XmnI GAAnn'nnTTC

Cuts at:      0    1112    1257 
   Size:       1112     145
 

 Enzymes that do cut:

     AccI    AlwNI     ApoI     AseI     AvaI    BamHI    BanII    BfrBI
     BmrI     BplI     BpmI   Bpu10I     BsaI    BseRI     BsgI Bsp1286I
    BspMI    BsrFI    BssSI    BstYI  BstZ17I     BtgI     BtsI     DraI
     EciI  Eco57MI EcoO109I     FalI    Hin4I     NcoI     NdeI     NsiI
     PasI    PpuMI     PsrI     PstI     SfcI     SmaI     StyI    TaqII
  Tth111I     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI
    AsiSI    AvrII     BaeI     BanI     BbeI     BbsI    BbvCI     BcgI
    BciVI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmtI
    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrDI
    BsrGI   BssHII   BstAPI    BstBI   BstEII    BstXI   Bsu36I    BtgZI
     ClaI    CspCI   DraIII     DrdI     EaeI     EagI     EarI  EcoICRI
    EcoNI    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI   NgoMIV     NheI     NotI     NruI
     NspI     PacI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PshAI     PsiI   PspOMI    PspXI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI     TatI     TstI     XbaI     XhoI     ZraI
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