Restriction map for pF1KE0426
##################################
# NO CUT ENZYMES (  149 enzymes) #
##################################

Acc65I      AccII       AccIII      AclI        AfaI        
AflII       AflIII      AgeI        Alw44I      AlwNI       
AocI        Aor51HI     ApaLI       AscI        AseI        
AsnI        Asp718I     AspHI       AspI        AviII       
AvrII       BalI        BbeI        BbrPI       BcgI        
BciVI       BclI        BfaI        BfrI        BglI        
BglII       BlnI        BpmI        BsaAI       BsaBI       
BsaWI       BseAI       BsiEI       BsiHKAI     BsiWI       
BsmFI       BsmI        Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrBI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstUI       BstXI       BstZ17I     Bsu36I      
CfoI        ClaI        CpoI        DraIII      EaeI        
EagI        EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO65I     EcoRV       EcoT22I     FbaI        
FseI        FspI        HaeII       HhaI        HinP1I      
HincII      HindII      HpaI        KasI        KpnI        
KspI        MaeI        MamI        MfeI        MluI        
MluNI       MroI        MscI        MunI        MvnI        
NaeI        NarI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PstI        PvuI        RcaI        RsaI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfcI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        Tth111I     XbaI        
XcmI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AatII         886  GACGTC               GACGT^C
AccI          129  GTCTAC               GT^[AC][GT]AC
AcyI          886  GACGTC               G[GA]^CG[CT]C
AhdI          349  GACTCAGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI          335  GGGCCC               GGGCC^C
Asp700I       423  GAAGGACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI         349  GACTCAGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI          640  CCCGAG               C^[CT]CG[GA]G
AvaII         196  GGACC                G^G[AT]CC
BamHI         662  GGATCC               G^GATCC
BbsI          357  GTCTTC               GAAGAC(2/6)
BbvCI         315  CCTCAGC              CCTCAGC(-5/-2)
BcnI          666  CCGGG                CC^[GC]GG
BlpI          805  GCTAAGC              GC^T[ACGT]AGC
Bpu1102I      805  GCTAAGC              GC^T[ACGT]AGC
BpuAI         357  GTCTTC               GAAGAC(2/6)
BsaHI         886  GACGTC               G[GA]^CG[CT]C
BsaI          898  GAGACC               GGTCTC(1/5)
BsgI          477  CTGCAC               GTGCAG(16/14)
BsmBI         292  GAGACG               CGTCTC(1/5)
BsoBI         640  CCCGAG               C^[CT]CG[GA]G
BsrFI         481  ACCGGC               [GA]^CCGG[CT]
BstYI         662  GGATCC               [GA]^GATC[CT]
CelII         805  GCTAAGC              GC^T[ACGT]AGC
Cfr10I        481  ACCGGC               [GA]^CCGG[CT]
DraI          595  TTTAAA               TTT^AAA
DraII         334  GGGGCCC              [GA]G^G[ACGT]CC[CT]
DrdI          197  GACCCCAGTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI          470  CCACGG               C^C[GA][CT]GG
Eam1105I      349  GACTCAGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I      334  GGGGCCC              [GA]G^G[ACGT]CC[CT]
EcoRI         827  GAATTC               G^AATTC
EcoT14I       689  CCAAGG               C^C[AT][AT]GG
FauI          947  GCGGG                CCCGC(4/6)
HgaI          294  GACGC                GACGC(5/10)
Hin1I         886  GACGTC               G[GA]^CG[CT]C
HindIII       565  AAGCTT               A^AGCTT
MaeIII        884  GTGAC                ^GT[ACGT]AC
MflI          662  GGATCC               [GA]^GATC[CT]
NciI          666  CCGGG                CC^[GC]GG
NspI          504  GCATGT               [GA]CATG^[CT]
StyI          689  CCAAGG               C^C[AT][AT]GG
Tsp45I        884  GTGAC                ^GT[GC]AC
XhoII         662  GGATCC               [GA]^GATC[CT]
XmnI          423  GAAGGACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

BanI          484  GGCACC               G^G[CT][GA]CC
             1025  GGCACC              
BseRI          19  CTCCTC               GAGGAG(10/8)
             1085  CTCCTC              
BspMI         297  GCAGGT               ACCTGC(4/8)
              404  GCAGGT              
BsrDI         707  GCAATG               GCAATG(2/0)
              921  GCAATG              
BstF5I        312  CATCC                GGATG(2/0)
              968  GGATG               
Cac8I          96  GCCTGC               GC[ACGT]^[ACGT]GC
              943  GCCAGC              
DpnII         663  GATC                 ^GATC
              699  GATC                
FokI          312  CATCC                GGATG(9/13)
              968  GGATG               
HapII         482  CCGG                 C^CGG
              666  CCGG                
HpaII         482  CCGG                 C^CGG
              666  CCGG                
HphI          669  GGTGA                GGTGA(8/7)
              792  GGTGA               
MaeII         887  ACGT                 A^CGT
             1067  ACGT                
MboI          663  GATC                 ^GATC
              699  GATC                
MseI          596  TTAA                 T^TAA
             1118  TTAA                
MspI          482  CCGG                 C^CGG
              666  CCGG                
NdeII         663  GATC                 ^GATC
              699  GATC                
PleI          349  GACTC                GAGTC(4/5)
              622  GACTC               
PvuII         515  CAGCTG               CAG^CTG
              811  CAGCTG              
Sau3AI        663  GATC                 ^GATC
              699  GATC                
SfaNI         928  GATGC                GCATC(5/9)
              305  GCATC               
TfiI            9  GATTC                G^A[AT]TC
             1079  GAATC               
Tru9I         596  TTAA                 T^TAA
             1118  TTAA                


#########################################
# 3 OR MORE CUT ENZYMES (   42 enzymes) #
#########################################

AciI        AcsI        AluI        AlwI        ApoI        
BanII       BbvI        BmyI        BsaJI       BsiYI       
BslI        BsmAI       Bsp1286I    BsrI        BssKI       
BstNI       Cfr13I      DdeI        EarI        EcoRII      
Fnu4HI      HaeIII      HinfI       ItaI        Ksp632I     
MboII       MnlI        MslI        MspA1I      MvaI        
MwoI        NlaIII      NlaIV       PflMI       Sau96I      
ScrFI       TaqI        TseI        Tsp509I     TspRI       
TthHB8I     Van91I      
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