Restriction map for pF1KE0426
 (Linear) (Six-base) MAPSORT of: KIEE0426.seq  Check: 1022  from: 1  to: 1149



KIEE0426.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:14  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     288    1149 
   Size:        288     861
 
AatII G_ACGT'C

Cuts at:      0     890    1149 
   Size:        890     259
 
AccI GT'mk_AC

Cuts at:      0     130    1149 
   Size:        130    1019
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     344     614    1149 
   Size:        344     270     535

  Fragments arranged by size: 

                 535     344     270
 
AhdI GACnn_n'nnGTC

Cuts at:      0     354    1149 
   Size:        354     795
 
AleI CACnn'nnGTG

Cuts at:      0     490     682    1149 
   Size:        490     192     467

  Fragments arranged by size: 

                 490     467     192
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     700     944    1149 
   Size:        700     244     205

  Fragments arranged by size: 

                 700     244     205
 
ApaI G_GGCC'C

Cuts at:      0     339    1149 
   Size:        339     810
 
ApoI r'AATT_y

Cuts at:      0     652     775     827    1149 
   Size:        652     123      52     322

  Fragments arranged by size: 

                 652     322     123      52
 
AvaI C'yCGr_G

Cuts at:      0     640    1149 
   Size:        640     509
 
BamHI G'GATC_C

Cuts at:      0     662    1149 
   Size:        662     487
 
BanI G'GyrC_C

Cuts at:      0     484    1025    1149 
   Size:        484     541     124

  Fragments arranged by size: 

                 541     484     124
 
BanII G_rGCy'C

Cuts at:      0      20      67     339     799    1149 
   Size:         20      47     272     460     350

  Fragments arranged by size: 

                 460     350     272      47      20
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     350    1149 
   Size:        350     799
 
BbvCI CC'TCA_GC

Cuts at:      0     316    1149 
   Size:        316     833
 
BlpI GC'TnA_GC

Cuts at:      0     806    1149 
   Size:        806     343
 
Bme1580I G_kGCm'C

Cuts at:      0      78     339     689    1028    1149 
   Size:         78     261     350     339     121

  Fragments arranged by size: 

                 350     339     261     121      78
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     120     195    1149 
   Size:        120      75     954

  Fragments arranged by size: 

                 954     120      75
 
Bpu10I CC'TnA_GC

Cuts at:      0     316    1149 
   Size:        316     833
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     892    1149 
   Size:        892     257
 
BsaHI Gr'CG_yC

Cuts at:      0     887    1149 
   Size:        887     262
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      10    1076    1149 
   Size:         10    1066      73

  Fragments arranged by size: 

                1066      73      10
 
BseYI C'CCAG_C

Cuts at:      0     932    1149 
   Size:        932     217
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     462    1149 
   Size:        462     687
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     286    1149 
   Size:        286     863
 
Bsp1286I G_dGCh'C

Cuts at:      0      20      67      78     339     689     799    1028    1149 
   Size:         20      47      11     261     350     110     229     121

  Fragments arranged by size: 

                 350     261     229     121     110      47      20      11
            
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     288     395    1149 
   Size:        288     107     754

  Fragments arranged by size: 

                 754     288     107
 
BsrDI GCAATG_nn'

Cuts at:      0     714     928    1149 
   Size:        714     214     221

  Fragments arranged by size: 

                 714     221     214
 
BsrFI r'CCGG_y

Cuts at:      0     481    1149 
   Size:        481     668
 
BstYI r'GATC_y

Cuts at:      0     662    1149 
   Size:        662     487
 
BtgI C'CryG_G

Cuts at:      0     470    1149 
   Size:        470     679
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     941    1149 
   Size:        941     208
 
BtsI GCAGTG_nn'

Cuts at:      0     220     522    1149 
   Size:        220     302     627

  Fragments arranged by size: 

                 627     302     220
 
DraI TTT'AAA

Cuts at:      0     597    1149 
   Size:        597     552
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     203    1149 
   Size:        203     946
 
EarI CTCTTCn'nnn_

Cuts at:      0      28      37     230     970    1149 
   Size:         28       9     193     740     179

  Fragments arranged by size: 

                 740     193     179      28       9
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     344     614    1149 
   Size:        344     270     535

  Fragments arranged by size: 

                 535     344     270
 
EcoO109I rG'GnC_Cy

Cuts at:      0     335    1149 
   Size:        335     814
 
EcoRI G'AATT_C

Cuts at:      0     827    1149 
   Size:        827     322
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     171    1149 
   Size:        171     978
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     340     372    1149 
   Size:        340      32     777

  Fragments arranged by size: 

                 777     340      32
 
HindIII A'AGCT_T

Cuts at:      0     565    1149 
   Size:        565     584
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     676    1149 
   Size:        676     473
 
MslI CAynn'nnrTG

Cuts at:      0     490     682     724    1149 
   Size:        490     192      42     425

  Fragments arranged by size: 

                 490     425     192      42
 
MspA1I CmG'CkG

Cuts at:      0     517     813     947    1149 
   Size:        517     296     134     202

  Fragments arranged by size: 

                 517     296     202     134
 
NspI r_CATG'y

Cuts at:      0     508    1149 
   Size:        508     641
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     157     695    1035    1149 
   Size:        157     538     340     114

  Fragments arranged by size: 

                 538     340     157     114
 
PfoI T'CCnGG_A

Cuts at:      0     746    1149 
   Size:        746     403
 
PspOMI G'GGCC_C

Cuts at:      0     335    1149 
   Size:        335     814
 
PvuII CAG'CTG

Cuts at:      0     517     813    1149 
   Size:        517     296     336

  Fragments arranged by size: 

                 517     336     296
 
StyI C'CwwG_G

Cuts at:      0     689    1149 
   Size:        689     460
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     694     869    1023    1149 
   Size:        694     175     154     126

  Fragments arranged by size: 

                 694     175     154     126
 
XmnI GAAnn'nnTTC

Cuts at:      0     427    1149 
   Size:        427     722
 
ZraI GAC'GTC

Cuts at:      0     888    1149 
   Size:        888     261
 

 Enzymes that do cut:

     AarI    AatII     AccI     AcuI     AhdI     AleI     AlfI     ApaI
     ApoI     AvaI    BamHI     BanI    BanII     BbsI    BbvCI     BlpI
 Bme1580I     BmrI   Bpu10I     BsaI    BsaHI    BseRI    BseYI     BsgI
    BsmBI Bsp1286I    BspMI    BsrDI    BsrFI    BstYI     BtgI    BtgZI
     BtsI     DraI     DrdI     EarI  Eco57MI EcoO109I    EcoRI     FalI
    Hin4I  HindIII     MmeI     MslI   MspA1I     NspI    PflMI     PfoI
   PspOMI    PvuII     StyI    TaqII     XmnI     ZraI

 Enzymes that do not cut: 

   Acc65I     AclI     AfeI    AflII   AflIII     AgeI     AloI    AlwNI
    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI     BbeI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII    BmgBI     BmtI     BplI
     BpmI    BpuEI    BsaAI    BsaBI    BsaWI    BsaXI    BsiEI  BsiHKAI
    BsiWI     BsmI    BspEI    BspHI    BsrBI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     ClaI    CspCI
   DraIII     EaeI     EagI     EciI  EcoICRI    EcoNI    EcoRV     FseI
     FspI    FspAI    HaeII   HincII     HpaI     KasI     KpnI     MfeI
     MluI     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI     PciI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI    PspXI     PsrI     PstI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
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