Restriction map for pF1KB6497
 (Linear) (Six-base) MAPSORT of: KIBB6497.seq  Check: 1503  from: 1  to: 1053



KIBB6497.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:47  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     534     813    1053 
   Size:        534     279     240

  Fragments arranged by size: 

                 534     279     240
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     523    1053 
   Size:        523     530
 
AfeI AGC'GCT

Cuts at:      0     841    1053 
   Size:        841     212
 
AlwNI CAG_nnn'CTG

Cuts at:      0     964    1053 
   Size:        964      89
 
ApaI G_GGCC'C

Cuts at:      0     672    1053 
   Size:        672     381
 
AvaI C'yCGr_G

Cuts at:      0     508     622    1053 
   Size:        508     114     431

  Fragments arranged by size: 

                 508     431     114
 
AvrII C'CTAG_G

Cuts at:      0     672    1053 
   Size:        672     381
 
BanI G'GyrC_C

Cuts at:      0     185    1053 
   Size:        185     868
 
BanII G_rGCy'C

Cuts at:      0     160     425     672    1007    1053 
   Size:        160     265     247     335      46

  Fragments arranged by size: 

                 335     265     247     160      46
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     409     443    1053 
   Size:        409      34     610

  Fragments arranged by size: 

                 610     409      34
 
BglII A'GATC_T

Cuts at:      0     737    1053 
   Size:        737     316
 
Bme1580I G_kGCm'C

Cuts at:      0     672    1053 
   Size:        672     381
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      97     405    1053 
   Size:         97     308     648

  Fragments arranged by size: 

                 648     308      97
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     304    1053 
   Size:        304     749
 
Bpu10I CC'TnA_GC

Cuts at:      0     139     634    1053 
   Size:        139     495     419

  Fragments arranged by size: 

                 495     419     139
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     781    1053 
   Size:        781     272
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     187    1053 
   Size:        187     866
 
BsaAI yAC'GTr

Cuts at:      0     369    1053 
   Size:        369     684
 
BsaBI GATnn'nnATC

Cuts at:      0     913    1015    1053 
   Size:        913     102      38

  Fragments arranged by size: 

                 913     102      38
 
BsaWI w'CCGG_w

Cuts at:      0    1007    1053 
   Size:       1007      46
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      52      86     508    1009    1053 
   Size:         52      34     422     501      44

  Fragments arranged by size: 

                 501     422      52      44      34
 
BseYI C'CCAG_C

Cuts at:      0     147    1053 
   Size:        147     906
 
BsiEI CG_ry'CG

Cuts at:      0     296    1053 
   Size:        296     757
 
BsiHKAI G_wGCw'C

Cuts at:      0     263     683    1007    1053 
   Size:        263     420     324      46

  Fragments arranged by size: 

                 420     324     263      46
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     759    1053 
   Size:        759     294
 
Bsp1286I G_dGCh'C

Cuts at:      0     160     263     425     672     683    1007    1053 
   Size:        160     103     162     247      11     324      46

  Fragments arranged by size: 

                 324     247     162     160     103      46      11
 
BspEI T'CCGG_A

Cuts at:      0    1007    1053 
   Size:       1007      46
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     243     534     548     813    1053 
   Size:        243     291      14     265     240

  Fragments arranged by size: 

                 291     265     243     240      14
 
BsrBI CCG'CTC

Cuts at:      0     103    1053 
   Size:        103     950
 
BsrFI r'CCGG_y

Cuts at:      0     196    1053 
   Size:        196     857
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     807    1053 
   Size:        807     246
 
BstBI TT'CG_AA

Cuts at:      0     131    1053 
   Size:        131     922
 
BstYI r'GATC_y

Cuts at:      0     218     737    1010    1053 
   Size:        218     519     273      43

  Fragments arranged by size: 

                 519     273     218      43
 
Bsu36I CC'TnA_GG

Cuts at:      0     853    1053 
   Size:        853     200
 
BtgI C'CryG_G

Cuts at:      0     656     698    1053 
   Size:        656      42     355

  Fragments arranged by size: 

                 656     355      42
 
EaeI y'GGCC_r

Cuts at:      0     201     458     695    1053 
   Size:        201     257     237     358

  Fragments arranged by size: 

                 358     257     237     201
 
EarI CTCTTCn'nnn_

Cuts at:      0     995    1053 
   Size:        995      58
 
EcoICRI GAG'CTC

Cuts at:      0    1005    1053 
   Size:       1005      48
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     304     523    1053 
   Size:        304     219     530

  Fragments arranged by size: 

                 530     304     219
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     820    1053 
   Size:        820     233
 
EcoO109I rG'GnC_Cy

Cuts at:      0     602     669     961    1053 
   Size:        602      67     292      92

  Fragments arranged by size: 

                 602     292      92      67
 
HaeII r_GCGC'y

Cuts at:      0     843    1053 
   Size:        843     210
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     387    1053 
   Size:        387     666
 
MspA1I CmG'CkG

Cuts at:      0     905    1053 
   Size:        905     148
 
PasI CC'CwG_GG

Cuts at:      0     964    1053 
   Size:        964      89
 
PpuMI rG'GwC_Cy

Cuts at:      0     602    1053 
   Size:        602     451
 
PspOMI G'GGCC_C

Cuts at:      0     668    1053 
   Size:        668     385
 
PstI C_TGCA'G

Cuts at:      0     290     545     824    1053 
   Size:        290     255     279     229

  Fragments arranged by size: 

                 290     279     255     229
 
PvuI CG_AT'CG

Cuts at:      0     296    1053 
   Size:        296     757
 
PvuII CAG'CTG

Cuts at:      0     905    1053 
   Size:        905     148
 
SacI G_AGCT'C

Cuts at:      0    1007    1053 
   Size:       1007      46
 
SanDI GG'GwC_CC

Cuts at:      0     602    1053 
   Size:        602     451
 
SapI GCTCTTCn'nnn_

Cuts at:      0     995    1053 
   Size:        995      58
 
SbfI CC_TGCA'GG

Cuts at:      0     290     545    1053 
   Size:        290     255     508

  Fragments arranged by size: 

                 508     290     255
 
SexAI A'CCwGG_T

Cuts at:      0     353    1053 
   Size:        353     700
 
SfcI C'TryA_G

Cuts at:      0      84     286     397     541     820    1053 
   Size:         84     202     111     144     279     233

  Fragments arranged by size: 

                 279     233     202     144     111      84
 
SmlI C'TyrA_G

Cuts at:      0     796    1053 
   Size:        796     257
 
StyI C'CwwG_G

Cuts at:      0     672    1034    1053 
   Size:        672     362      19

  Fragments arranged by size: 

                 672     362      19
 
TatI w'GTAC_w

Cuts at:      0     953    1053 
   Size:        953     100
 
XmnI GAAnn'nnTTC

Cuts at:      0      52    1053 
   Size:         52    1001
 

 Enzymes that do cut:

     AarI     AcuI     AfeI    AlwNI     ApaI     AvaI    AvrII     BanI
    BanII     BcgI    BglII Bme1580I     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaWI    BseRI    BseYI    BsiEI  BsiHKAI
    BsmBI Bsp1286I    BspEI    BspMI    BsrBI    BsrFI   BstAPI    BstBI
    BstYI   Bsu36I     BtgI     EaeI     EarI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    HaeII     MmeI   MspA1I     PasI    PpuMI   PspOMI     PstI
     PvuI    PvuII     SacI    SanDI     SapI     SbfI    SexAI     SfcI
     SmlI     StyI     TatI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     AloI    ApaLI     ApoI     AscI     AseI    AsiSI
     BaeI    BamHI     BbeI     BbsI    BbvCI    BciVI     BclI    BfrBI
     BglI     BlpI    BmgBI     BmrI     BmtI    BsaHI    BsaXI     BsgI
    BsiWI     BsmI    BspHI    BsrDI    BsrGI   BssHII    BssSI   BstEII
    BstXI  BstZ17I    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EagI     EciI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MscI     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI    PspXI     PsrI
    RsrII    SacII     SalI     ScaI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI
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