Restriction map for pF1KB7860
 (Linear) (Six-base) MAPSORT of: KIBB7860.seq  Check: 4847  from: 1  to: 1797



KIBB7860.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:50  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1181    1797 
   Size:       1181     616
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      55     155     745     806    1310    1522    1551    1678 
   Size:         55     100     590      61     504     212      29     127

Cuts at:   1678    1797 
   Size:        119

  Fragments arranged by size: 

                 590     504     212     127     119     100      61      55
                  29
 
AleI CACnn'nnGTG

Cuts at:      0    1442    1797 
   Size:       1442     355
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      31    1797 
   Size:         31    1766
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1099    1131    1797 
   Size:       1099      32     666

  Fragments arranged by size: 

                1099     666      32
 
ApaLI G'TGCA_C

Cuts at:      0    1762    1797 
   Size:       1762      35
 
ApoI r'AATT_y

Cuts at:      0     441     592     619     889    1396    1797 
   Size:        441     151      27     270     507     401

  Fragments arranged by size: 

                 507     441     401     270     151      27
 
AvaI C'yCGr_G

Cuts at:      0      44    1797 
   Size:         44    1753
 
BanI G'GyrC_C

Cuts at:      0     261    1797 
   Size:        261    1536
 
BanII G_rGCy'C

Cuts at:      0      68    1797 
   Size:         68    1729
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     143     456    1028    1099    1797 
   Size:        143     313     572      71     698

  Fragments arranged by size: 

                 698     572     313     143      71
 
BbvCI CC'TCA_GC

Cuts at:      0     389    1797 
   Size:        389    1408
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1233    1797 
   Size:       1233     564
 
BclI T'GATC_A

Cuts at:      0     923     987    1797 
   Size:        923      64     810

  Fragments arranged by size: 

                 923     810      64
 
BlpI GC'TnA_GC

Cuts at:      0     548    1797 
   Size:        548    1249
 
Bme1580I G_kGCm'C

Cuts at:      0    1766    1797 
   Size:       1766      31
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     619    1797 
   Size:        619    1178
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     137     541     791    1631    1797 
   Size:        137     404     250     840     166

  Fragments arranged by size: 

                 840     404     250     166     137
 
Bpu10I CC'TnA_GC

Cuts at:      0     389    1797 
   Size:        389    1408
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1104    1797 
   Size:       1104     693
 
BsaAI yAC'GTr

Cuts at:      0      15    1797 
   Size:         15    1782
 
BsaWI w'CCGG_w

Cuts at:      0    1145    1430    1797 
   Size:       1145     285     367

  Fragments arranged by size: 

                1145     367     285
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      13      43    1099    1129    1522    1552    1797 
   Size:         13      30    1056      30     393      30     245

  Fragments arranged by size: 

                1056     393     245      30      30      30      13
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     406     598    1120    1797 
   Size:        406     192     522     677

  Fragments arranged by size: 

                 677     522     406     192
 
BseYI C'CCAG_C

Cuts at:      0       3    1797 
   Size:          3    1794
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1575    1797 
   Size:       1575     222
 
BsiHKAI G_wGCw'C

Cuts at:      0    1002    1549    1595    1766    1797 
   Size:       1002     547      46     171      31

  Fragments arranged by size: 

                1002     547     171      46      31
 
BsmI GAATG_Cn'

Cuts at:      0     134    1720    1797 
   Size:        134    1586      77

  Fragments arranged by size: 

                1586     134      77
 
Bsp1286I G_dGCh'C

Cuts at:      0      68    1002    1549    1595    1766    1797 
   Size:         68     934     547      46     171      31

  Fragments arranged by size: 

                 934     547     171      68      46      31
 
BspEI T'CCGG_A

Cuts at:      0    1145    1430    1797 
   Size:       1145     285     367

  Fragments arranged by size: 

                1145     367     285
 
BspHI T'CATG_A

Cuts at:      0     255     855    1269    1797 
   Size:        255     600     414     528

  Fragments arranged by size: 

                 600     528     414     255
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1181    1797 
   Size:       1181     616
 
BsrDI GCAATG_nn'

Cuts at:      0     562    1797 
   Size:        562    1235
 
BsrGI T'GTAC_A

Cuts at:      0     203    1797 
   Size:        203    1594
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     639    1797 
   Size:        639    1158
 
BstYI r'GATC_y

Cuts at:      0     774    1797 
   Size:        774    1023
 
BtgI C'CryG_G

Cuts at:      0     226    1779    1797 
   Size:        226    1553      18

  Fragments arranged by size: 

                1553     226      18
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1232    1797 
   Size:       1232     565
 
BtsI GCAGTG_nn'

Cuts at:      0    1212    1413    1797 
   Size:       1212     201     384

  Fragments arranged by size: 

                1212     384     201
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     591     626    1029    1064    1797 
   Size:        591      35     403      35     733

  Fragments arranged by size: 

                 733     591     403      35      35
 
EaeI y'GGCC_r

Cuts at:      0    1389    1797 
   Size:       1389     408
 
EarI CTCTTCn'nnn_

Cuts at:      0     118     721    1797 
   Size:        118     603    1076

  Fragments arranged by size: 

                1076     603     118
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      55     137     155     541     745     791     806    1310 
   Size:         55      82      18     386     204      46      15     504

Cuts at:   1310    1522    1551    1631    1678    1797 
   Size:        212      29      80      47     119

  Fragments arranged by size: 

                 504     386     212     204     119      82      80      55
                  47      46      29      18      15
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1230    1797 
   Size:       1230     567
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     496    1797 
   Size:        496    1301
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      13      45     250     282     972    1004    1412    1444 
   Size:         13      32     205      32     690      32     408      32

Cuts at:   1444    1677    1709    1797 
   Size:        233      32      88

  Fragments arranged by size: 

                 690     408     233     205      88      32      32      32
                  32      32      13
 
HindIII A'AGCT_T

Cuts at:      0     787    1797 
   Size:        787    1010
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     260    1797 
   Size:        260    1537
 
MscI TGG'CCA

Cuts at:      0    1391    1797 
   Size:       1391     406
 
MslI CAynn'nnrTG

Cuts at:      0      13    1070    1187    1217    1442    1797 
   Size:         13    1057     117      30     225     355

  Fragments arranged by size: 

                1057     355     225     117      30      13
 
MspA1I CmG'CkG

Cuts at:      0     463     710    1797 
   Size:        463     247    1087

  Fragments arranged by size: 

                1087     463     247
 
NcoI C'CATG_G

Cuts at:      0    1779    1797 
   Size:       1779      18
 
NdeI CA'TA_TG

Cuts at:      0     920    1797 
   Size:        920     877
 
NspI r_CATG'y

Cuts at:      0      12    1797 
   Size:         12    1785
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1387    1797 
   Size:       1387     410
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1099    1131    1797 
   Size:       1099      32     666

  Fragments arranged by size: 

                1099     666      32
 
PvuII CAG'CTG

Cuts at:      0     710    1797 
   Size:        710    1087
 
SapI GCTCTTCn'nnn_

Cuts at:      0     721    1797 
   Size:        721    1076
 
SfcI C'TryA_G

Cuts at:      0     835    1797 
   Size:        835     962
 
SmaI CCC'GGG

Cuts at:      0      46    1797 
   Size:         46    1751
 
StuI AGG'CCT

Cuts at:      0    1312    1564    1583    1797 
   Size:       1312     252      19     214

  Fragments arranged by size: 

                1312     252     214      19
 
StyI C'CwwG_G

Cuts at:      0     480    1779    1797 
   Size:        480    1299      18

  Fragments arranged by size: 

                1299     480      18
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     943     985    1797 
   Size:        943      42     812

  Fragments arranged by size: 

                 943     812      42
 
TatI w'GTAC_w

Cuts at:      0     203    1797 
   Size:        203    1594
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     759     791    1797 
   Size:        759      32    1006

  Fragments arranged by size: 

                1006     759      32
 
XbaI T'CTAG_A

Cuts at:      0     445    1797 
   Size:        445    1352
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1704    1797 
   Size:       1704      93
 
XmaI C'CCGG_G

Cuts at:      0      44    1797 
   Size:         44    1753
 
XmnI GAAnn'nnTTC

Cuts at:      0     619    1797 
   Size:        619    1178
 

 Enzymes that do cut:

     AarI     AcuI     AleI     AlfI     AloI    ApaLI     ApoI     AvaI
     BanI    BanII     BbsI    BbvCI    BciVI     BclI     BlpI Bme1580I
     BmrI     BpmI   Bpu10I     BsaI    BsaAI    BsaWI    BsaXI    BseRI
    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI    BspMI
    BsrDI    BsrGI   BstAPI    BstYI     BtgI    BtgZI     BtsI    CspCI
     EaeI     EarI  Eco57MI    EcoNI     FalI    Hin4I  HindIII     MmeI
     MscI     MslI   MspA1I     NcoI     NdeI     NspI    PflMI     PpiI
    PvuII     SapI     SfcI     SmaI     StuI     StyI    TaqII     TatI
     TstI     XbaI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI    AlwNI     ApaI     AscI     AseI    AsiSI    AvrII     BaeI
    BamHI     BbeI     BcgI    BfrBI     BglI    BglII    BmgBI     BmtI
     BplI    BpuEI    BsaBI    BsaHI    BsiEI    BsiWI    BsmBI    BsrBI
    BsrFI   BssHII    BssSI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
     ClaI     DraI   DraIII     DrdI     EagI     EciI  EcoICRI EcoO109I
    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII   HincII     HpaI
     KasI     KpnI     MfeI     MluI     NaeI     NarI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI     PciI     PfoI     PmeI
     PmlI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     SwaI  Tth111I     XhoI     ZraI
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