Restriction map for pF1KE0042
 (Linear) (Six-base) MAPSORT of: KIEE0042.seq  Check: 2041  from: 1  to: 1533



KIEE0042.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:09  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     289    1533 
   Size:        289    1244
 
AflII C'TTAA_G

Cuts at:      0    1393    1533 
   Size:       1393     140
 
AgeI A'CCGG_T

Cuts at:      0     399    1533 
   Size:        399    1134
 
AleI CACnn'nnGTG

Cuts at:      0     400    1533 
   Size:        400    1133
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     307     339    1533 
   Size:        307      32    1194

  Fragments arranged by size: 

                1194     307      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     693     882    1440    1533 
   Size:        693     189     558      93

  Fragments arranged by size: 

                 693     558     189      93
 
AvaI C'yCGr_G

Cuts at:      0     671    1533 
   Size:        671     862
 
BanI G'GyrC_C

Cuts at:      0     402    1533 
   Size:        402    1131
 
BanII G_rGCy'C

Cuts at:      0     260    1373    1418    1448    1533 
   Size:        260    1113      45      30      85

  Fragments arranged by size: 

                1113     260      85      45      30
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     178     902    1533 
   Size:        178     724     631

  Fragments arranged by size: 

                 724     631     178
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1261    1295    1533 
   Size:       1261      34     238

  Fragments arranged by size: 

                1261     238      34
 
BglII A'GATC_T

Cuts at:      0     545    1533 
   Size:        545     988
 
Bme1580I G_kGCm'C

Cuts at:      0     296     407    1533 
   Size:        296     111    1126

  Fragments arranged by size: 

                1126     296     111
 
BmgBI CAC'GTC

Cuts at:      0     486    1533 
   Size:        486    1047
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     201    1533 
   Size:        201    1332
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1048    1357    1462    1533 
   Size:       1048     309     105      71

  Fragments arranged by size: 

                1048     309     105      71
 
Bpu10I CC'TnA_GC

Cuts at:      0    1075    1412    1533 
   Size:       1075     337     121

  Fragments arranged by size: 

                1075     337     121
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     191     222     628    1218    1533 
   Size:        191      31     406     590     315

  Fragments arranged by size: 

                 590     406     315     191      31
 
BsaAI yAC'GTr

Cuts at:      0     660    1533 
   Size:        660     873
 
BsaHI Gr'CG_yC

Cuts at:      0    1121    1533 
   Size:       1121     412
 
BsaWI w'CCGG_w

Cuts at:      0     231     399    1533 
   Size:        231     168    1134

  Fragments arranged by size: 

                1134     231     168
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1430    1460    1533 
   Size:       1430      30      73

  Fragments arranged by size: 

                1430      73      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     228     691     857    1264    1533 
   Size:        228     463     166     407     269

  Fragments arranged by size: 

                 463     407     269     228     166
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     756     784    1180    1533 
   Size:        756      28     396     353

  Fragments arranged by size: 

                 756     396     353      28
 
BsiHKAI G_wGCw'C

Cuts at:      0    1081    1373    1448    1533 
   Size:       1081     292      75      85

  Fragments arranged by size: 

                1081     292      85      75
 
BsmI GAATG_Cn'

Cuts at:      0     364    1533 
   Size:        364    1169
 
Bsp1286I G_dGCh'C

Cuts at:      0     260     296     407    1081    1373    1418    1448    1533 
   Size:        260      36     111     674     292      45      30      85

  Fragments arranged by size: 

                 674     292     260     111      85      45      36      30
            
 
BspHI T'CATG_A

Cuts at:      0    1308    1533 
   Size:       1308     225
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     701     867    1459    1533 
   Size:        701     166     592      74

  Fragments arranged by size: 

                 701     592     166      74
 
BsrFI r'CCGG_y

Cuts at:      0     399    1533 
   Size:        399    1134
 
BsrGI T'GTAC_A

Cuts at:      0     975    1533 
   Size:        975     558
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     693    1533 
   Size:        693     840
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     208    1533 
   Size:        208    1325
 
BstYI r'GATC_y

Cuts at:      0     545    1533 
   Size:        545     988
 
Bsu36I CC'TnA_GG

Cuts at:      0     901    1533 
   Size:        901     632
 
BtgI C'CryG_G

Cuts at:      0     146     245     369     407    1152    1533 
   Size:        146      99     124      38     745     381

  Fragments arranged by size: 

                 745     381     146     124      99      38
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     504    1533 
   Size:        504    1029
 
BtsI GCAGTG_nn'

Cuts at:      0    1521    1533 
   Size:       1521      12
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1198    1533 
   Size:       1198     335
 
EaeI y'GGCC_r

Cuts at:      0     210     242     836    1533 
   Size:        210      32     594     697

  Fragments arranged by size: 

                 697     594     210      32
 
EarI CTCTTCn'nnn_

Cuts at:      0      79     844    1241    1533 
   Size:         79     765     397     292

  Fragments arranged by size: 

                 765     397     292      79
 
EcoICRI GAG'CTC

Cuts at:      0    1371    1446    1533 
   Size:       1371      75      87

  Fragments arranged by size: 

                1371      87      75
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     289    1048    1357    1462    1533 
   Size:        289     759     309     105      71

  Fragments arranged by size: 

                 759     309     289     105      71
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     222    1533 
   Size:        222    1311
 
EcoO109I rG'GnC_Cy

Cuts at:      0     907    1200    1533 
   Size:        907     293     333

  Fragments arranged by size: 

                 907     333     293
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     141     975    1533 
   Size:        141     834     558

  Fragments arranged by size: 

                 834     558     141
 
HaeII r_GCGC'y

Cuts at:      0     433     798    1533 
   Size:        433     365     735

  Fragments arranged by size: 

                 735     433     365
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1243    1275    1533 
   Size:       1243      32     258

  Fragments arranged by size: 

                1243     258      32
 
HindIII A'AGCT_T

Cuts at:      0     300    1533 
   Size:        300    1233
 
MfeI C'AATT_G

Cuts at:      0      20    1231    1533 
   Size:         20    1211     302

  Fragments arranged by size: 

                1211     302      20
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1478    1533 
   Size:       1478      55
 
MscI TGG'CCA

Cuts at:      0     212     838    1533 
   Size:        212     626     695

  Fragments arranged by size: 

                 695     626     212
 
MslI CAynn'nnrTG

Cuts at:      0     400     454    1533 
   Size:        400      54    1079

  Fragments arranged by size: 

                1079     400      54
 
MspA1I CmG'CkG

Cuts at:      0     568    1533 
   Size:        568     965
 
NcoI C'CATG_G

Cuts at:      0     245     369    1152    1533 
   Size:        245     124     783     381

  Fragments arranged by size: 

                 783     381     245     124
 
NspI r_CATG'y

Cuts at:      0     810    1392    1533 
   Size:        810     582     141

  Fragments arranged by size: 

                 810     582     141
 
PasI CC'CwG_GG

Cuts at:      0    1179    1206    1533 
   Size:       1179      27     327

  Fragments arranged by size: 

                1179     327      27
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     312     370    1068    1533 
   Size:        312      58     698     465

  Fragments arranged by size: 

                 698     465     312      58
 
PpuMI rG'GwC_Cy

Cuts at:      0     907    1200    1533 
   Size:        907     293     333

  Fragments arranged by size: 

                 907     333     293
 
PshAI GACnn'nnGTC

Cuts at:      0     178    1533 
   Size:        178    1355
 
PstI C_TGCA'G

Cuts at:      0    1470    1533 
   Size:       1470      63
 
PvuII CAG'CTG

Cuts at:      0     568    1533 
   Size:        568     965
 
SacI G_AGCT'C

Cuts at:      0    1373    1448    1533 
   Size:       1373      75      85

  Fragments arranged by size: 

                1373      85      75
 
SanDI GG'GwC_CC

Cuts at:      0     907    1533 
   Size:        907     626
 
SbfI CC_TGCA'GG

Cuts at:      0    1470    1533 
   Size:       1470      63
 
ScaI AGT'ACT

Cuts at:      0     346    1533 
   Size:        346    1187
 
SfcI C'TryA_G

Cuts at:      0    1125    1466    1533 
   Size:       1125     341      67

  Fragments arranged by size: 

                1125     341      67
 
SgrAI Cr'CCGG_yG

Cuts at:      0     399    1533 
   Size:        399    1134
 
SmaI CCC'GGG

Cuts at:      0     673    1533 
   Size:        673     860
 
SmlI C'TyrA_G

Cuts at:      0    1393    1533 
   Size:       1393     140
 
SpeI A'CTAG_T

Cuts at:      0     469    1533 
   Size:        469    1064
 
StuI AGG'CCT

Cuts at:      0    1033    1288    1533 
   Size:       1033     255     245

  Fragments arranged by size: 

                1033     255     245
 
StyI C'CwwG_G

Cuts at:      0     245     369     512    1152    1217    1533 
   Size:        245     124     143     640      65     316

  Fragments arranged by size: 

                 640     316     245     143     124      65
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     121     568    1533 
   Size:        121     447     965

  Fragments arranged by size: 

                 965     447     121
 
TatI w'GTAC_w

Cuts at:      0     344     975    1533 
   Size:        344     631     558

  Fragments arranged by size: 

                 631     558     344
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      59      91     793     825    1533 
   Size:         59      32     702      32     708

  Fragments arranged by size: 

                 708     702      59      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     144    1533 
   Size:        144    1389
 
XmaI C'CCGG_G

Cuts at:      0     671    1533 
   Size:        671     862
 
XmnI GAAnn'nnTTC

Cuts at:      0     821    1533 
   Size:        821     712
 

 Enzymes that do cut:

     AcuI    AflII     AgeI     AleI     AloI    AlwNI     AvaI     BanI
    BanII     BbsI     BcgI    BglII Bme1580I    BmgBI     BplI     BpmI
   Bpu10I     BsaI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI     BsgI
  BsiHKAI     BsmI Bsp1286I    BspHI    BspMI    BsrFI    BsrGI   BstAPI
    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI     DrdI     EaeI
     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I     FalI    HaeII    Hin4I
  HindIII     MfeI     MmeI     MscI     MslI   MspA1I     NcoI     NspI
     PasI    PflMI    PpuMI    PshAI     PstI    PvuII     SacI    SanDI
     SbfI     ScaI     SfcI    SgrAI     SmaI     SmlI     SpeI     StuI
     StyI    TaqII     TatI     TstI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI   AflIII     AhdI
     AlfI     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI     BbeI    BbvCI    BciVI     BclI    BfrBI     BglI
     BlpI     BmrI     BmtI    BpuEI    BsaBI    BseYI    BsiEI    BsiWI
    BsmBI    BspEI    BsrBI    BsrDI   BssHII    BssSI    BstBI   BstEII
  BstZ17I     ClaI    CspCI     DraI   DraIII     EagI     EciI    EcoRI
    EcoRV     FseI     FspI    FspAI   HincII     HpaI     KasI     KpnI
     MluI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PciI     PfoI     PmeI     PmlI     PpiI     PsiI
   PspOMI    PspXI     PsrI     PvuI    RsrII    SacII     SalI     SapI
    SexAI     SfiI     SfoI     SgfI    SnaBI     SphI     SrfI     SspI
     SwaI  Tth111I     XbaI     XhoI     ZraI
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