Restriction map for pF1KB6503
 (Linear) (Six-base) MAPSORT of: KIBB6503.seq  Check: 2965  from: 1  to: 1074



KIBB6503.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:47  ..

Acc65I G'GTAC_C

Cuts at:      0     665    1074 
   Size:        665     409
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      20    1074 
   Size:         20    1054
 
AflIII A'CryG_T

Cuts at:      0     857    1074 
   Size:        857     217
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     844     876    1074 
   Size:        844      32     198

  Fragments arranged by size: 

                 844     198      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     425     496     756    1074 
   Size:        425      71     260     318

  Fragments arranged by size: 

                 425     318     260      71
 
ApaLI G'TGCA_C

Cuts at:      0     308    1074 
   Size:        308     766
 
BanI G'GyrC_C

Cuts at:      0     665    1074 
   Size:        665     409
 
BanII G_rGCy'C

Cuts at:      0     809    1074 
   Size:        809     265
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     639     901    1074 
   Size:        639     262     173

  Fragments arranged by size: 

                 639     262     173
 
BbvCI CC'TCA_GC

Cuts at:      0       5    1074 
   Size:          5    1069
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     883    1074 
   Size:        883     191
 
BfrBI ATG'CAT

Cuts at:      0     281    1074 
   Size:        281     793
 
Bme1580I G_kGCm'C

Cuts at:      0     305     312    1074 
   Size:        305       7     762

  Fragments arranged by size: 

                 762     305       7
 
BmgBI CAC'GTC

Cuts at:      0    1067    1074 
   Size:       1067       7
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      35     365     673     823     886    1024    1074 
   Size:         35     330     308     150      63     138      50

  Fragments arranged by size: 

                 330     308     150     138      63      50      35
 
Bpu10I CC'TnA_GC

Cuts at:      0       5    1074 
   Size:          5    1069
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     691    1074 
   Size:        691     383
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     979    1074 
   Size:        979      95
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     193     223    1074 
   Size:        193      30     851

  Fragments arranged by size: 

                 851     193      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      55     368    1074 
   Size:         55     313     706

  Fragments arranged by size: 

                 706     313      55
 
BsiHKAI G_wGCw'C

Cuts at:      0     312     620     917    1074 
   Size:        312     308     297     157

  Fragments arranged by size: 

                 312     308     297     157
 
BsmI GAATG_Cn'

Cuts at:      0     283    1074 
   Size:        283     791
 
Bsp1286I G_dGCh'C

Cuts at:      0     305     312     620     809     917    1074 
   Size:        305       7     308     189     108     157

  Fragments arranged by size: 

                 308     305     189     157     108       7
 
BspHI T'CATG_A

Cuts at:      0     231    1074 
   Size:        231     843
 
BsrBI CCG'CTC

Cuts at:      0      15    1074 
   Size:         15    1059
 
BsrFI r'CCGG_y

Cuts at:      0     715    1074 
   Size:        715     359
 
BstYI r'GATC_y

Cuts at:      0      86    1074 
   Size:         86     988
 
Bsu36I CC'TnA_GG

Cuts at:      0     503    1074 
   Size:        503     571
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     287    1074 
   Size:        287     787
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     531     566    1074 
   Size:        531      35     508

  Fragments arranged by size: 

                 531     508      35
 
DraIII CAC_nnn'GTG

Cuts at:      0     819    1074 
   Size:        819     255
 
EaeI y'GGCC_r

Cuts at:      0     464    1074 
   Size:        464     610
 
EarI CTCTTCn'nnn_

Cuts at:      0     347    1074 
   Size:        347     727
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      20      35     365     673     823     886    1024    1074 
   Size:         20      15     330     308     150      63     138      50

  Fragments arranged by size: 

                 330     308     150     138      63      50      20      15
            
 
EcoO109I rG'GnC_Cy

Cuts at:      0      45     422    1074 
   Size:         45     377     652

  Fragments arranged by size: 

                 652     377      45
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     210    1074 
   Size:        210     864
 
HaeII r_GCGC'y

Cuts at:      0     944    1074 
   Size:        944     130
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     379     411    1074 
   Size:        379      32     663

  Fragments arranged by size: 

                 663     379      32
 
KpnI G_GTAC'C

Cuts at:      0     669    1074 
   Size:        669     405
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     944    1074 
   Size:        944     130
 
MscI TGG'CCA

Cuts at:      0     466    1074 
   Size:        466     608
 
MslI CAynn'nnrTG

Cuts at:      0     982    1074 
   Size:        982      92
 
MspA1I CmG'CkG

Cuts at:      0     493     783     927    1074 
   Size:        493     290     144     147

  Fragments arranged by size: 

                 493     290     147     144
 
NaeI GCC'GGC

Cuts at:      0     717    1074 
   Size:        717     357
 
NgoMIV G'CCGG_C

Cuts at:      0     715    1074 
   Size:        715     359
 
NsiI A_TGCA'T

Cuts at:      0     283    1074 
   Size:        283     791
 
NspI r_CATG'y

Cuts at:      0     861     987    1074 
   Size:        861     126      87

  Fragments arranged by size: 

                 861     126      87
 
PciI A'CATG_T

Cuts at:      0     857    1074 
   Size:        857     217
 
PfoI T'CCnGG_A

Cuts at:      0     863    1074 
   Size:        863     211
 
PpuMI rG'GwC_Cy

Cuts at:      0     422    1074 
   Size:        422     652
 
PvuII CAG'CTG

Cuts at:      0     493     783     927    1074 
   Size:        493     290     144     147

  Fragments arranged by size: 

                 493     290     147     144
 
SapI GCTCTTCn'nnn_

Cuts at:      0     347    1074 
   Size:        347     727
 
SexAI A'CCwGG_T

Cuts at:      0     424    1074 
   Size:        424     650
 
SmlI C'TyrA_G

Cuts at:      0     670    1074 
   Size:        670     404
 
SpeI A'CTAG_T

Cuts at:      0     333    1074 
   Size:        333     741
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     809    1074 
   Size:        809     265
 
TatI w'GTAC_w

Cuts at:      0     200    1074 
   Size:        200     874
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     816    1074 
   Size:        816     258
 

 Enzymes that do cut:

   Acc65I     AcuI   AflIII     AloI    AlwNI    ApaLI     BanI    BanII
     BbsI    BbvCI    BciVI    BfrBI Bme1580I    BmgBI     BpmI   Bpu10I
    BpuEI     BsaI    BsaXI    BseRI  BsiHKAI     BsmI Bsp1286I    BspHI
    BsrBI    BsrFI    BstYI   Bsu36I    BtgZI    CspCI   DraIII     EaeI
     EarI  Eco57MI EcoO109I     FalI    HaeII    Hin4I     KpnI     MmeI
     MscI     MslI   MspA1I     NaeI   NgoMIV     NsiI     NspI     PciI
     PfoI    PpuMI    PvuII     SapI    SexAI     SmlI     SpeI    TaqII
     TatI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     ApaI     ApoI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI    BamHI     BbeI     BcgI     BclI     BglI    BglII
     BlpI     BmrI     BmtI     BplI    BsaAI    BsaBI    BsaHI    BsaWI
    BseYI     BsgI    BsiEI    BsiWI    BsmBI    BspEI    BspMI    BsrDI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I
     BtgI     BtsI     ClaI     DraI     DrdI     EagI     EciI  EcoICRI
    EcoNI    EcoRI    EcoRV     FseI     FspI    FspAI   HincII  HindIII
     HpaI     KasI     MfeI     MluI     NarI     NcoI     NdeI     NheI
     NotI     NruI     PacI     PasI    PflMI     PmeI     PmlI     PpiI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SbfI     ScaI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SphI     SrfI     SspI
     StuI     StyI     SwaI     TstI  Tth111I     XbaI     XhoI     XmaI
     XmnI     ZraI
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