Restriction map for pF1KB6458
##################################
# NO CUT ENZYMES (  147 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
AflII       AflIII      AgeI        Alw44I      AlwNI       
AocI        Aor51HI     ApaLI       AscI        AseI        
AsnI        Asp700I     Asp718I     AspHI       AspI        
AviII       AvrII       BanI        BbeI        BbrPI       
BbsI        BcgI        BciVI       BclI        BfrI        
BglI        BlnI        BlpI        Bpu1102I    BpuAI       
BsaAI       BsaHI       BsaWI       BseAI       BsiEI       
BsiHKAI     BsiWI       BsmBI       BsmI        Bsp1407I    
BspEI       BspHI       BspLU11I    BsrBI       BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstZ17I     Bsu36I      
CelII       Cfr10I      CpoI        DraI        DrdI        
EagI        EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO65I     EcoRI       EcoRV       EcoT22I     
FbaI        FseI        FspI        HaeII       HgaI        
Hin1I       HincII      HindII      HindIII     HpaI        
KasI        KpnI        KspI        MaeII       MfeI        
MluI        MroI        MseI        MunI        NaeI        
NarI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PvuI        PvuII       RcaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        Sse8387I    SspBI       
SspI        StuI        SwaI        Tru9I       Tth111I     
Van91I      XbaI        XcmI        XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AccI          223  GTCTAC               GT^[AC][GT]AC
AcsI          323  AAATTC               [GA]^AATT[CT]
AfaI          269  GTAC                 GT^AC
AhdI          187  GACCACCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI          717  GGGCCC               GGGCC^C
ApoI          323  AAATTC               [GA]^AATT[CT]
AspEI         187  GACCACCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI          203  CTCGGG               C^[CT]CG[GA]G
BalI         1018  TGGCCA               TGG^CCA
BamHI         645  GGATCC               G^GATCC
BanII         717  GGGCCC               G[GA]GC[CT]^C
BbvCI         316  CCTCAGC              CCTCAGC(-5/-2)
BcnI           73  CCCGG                CC^[GC]GG
BfaI           26  CTAG                 C^TAG
BglII         258  AGATCT               A^GATCT
BmyI          717  GGGCCC               G[AGT]GC[ACT]^C
BpmI          736  CTGGAG               CTGGAG(16/14)
BsaBI         640  GATGTGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI          342  GGTCTC               GGTCTC(1/5)
BsoBI         203  CTCGGG               C^[CT]CG[GA]G
Bsp1286I      717  GGGCCC               G[AGT]GC[ACT]^C
BspDI         331  ATCGAT               AT^CGAT
BspMI          58  GCAGGT               ACCTGC(4/8)
BsrDI         395  CATTGC               GCAATG(2/0)
BstXI         828  CCAGCCACATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI          331  ATCGAT               AT^CGAT
DraIII        809  CACACAGTG            CAC[ACGT][ACGT][ACGT]^GTG
EaeI         1018  TGGCCA               [CT]^GGCC[GA]
Eam1105I      187  GACCACCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT14I       843  CCATGG               C^C[AT][AT]GG
FauI           33  CCCGC                CCCGC(4/6)
HphI          600  GGTGA                GGTGA(8/7)
MaeI           26  CTAG                 C^TAG
MamI          640  GATGTGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI        1018  TGGCCA               TGG^CCA
MscI         1018  TGGCCA               TGG^CCA
MslI           82  CATCTGGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NciI           73  CCCGG                CC^[GC]GG
NcoI          843  CCATGG               C^CATGG
NspI          758  GCATGC               [GA]CATG^[CT]
RsaI          269  GTAC                 GT^AC
SmlI          448  CTCAAG               C^T[CT][GA]AG
SphI          758  GCATGC               GCATG^C
StyI          843  CCATGG               C^C[AT][AT]GG


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AccII          12  CGCG                 CG^CG
              379  CGCG                
AluI          485  AGCT                 AG^CT
              975  AGCT                
AlwI          646  GATCC                GGATC(4/5)
              645  GGATC               
AvaII         207  GGTCC                G^G[AT]CC
              690  GGTCC               
BbvI            7  GCAGC                GCAGC(8/12)
              214  GCTGC               
BseRI         472  GAGGAG               GAGGAG(10/8)
              547  GAGGAG              
BsgI          215  CTGCAC               GTGCAG(16/14)
              944  CTGCAC              
BsmAI         101  GAGAC                GTCTC(1/5)
              343  GTCTC               
BsmFI         847  GGGAC                GGGAC(10/14)
              691  GTCCC               
BstUI          12  CGCG                 CG^CG
              379  CGCG                
BstYI         258  AGATCT               [GA]^GATC[CT]
              645  GGATCC              
DsaI          338  CCACGG               C^C[GA][CT]GG
              843  CCATGG              
EarI          240  GAAGAG               CTCTTC(1/4)
              582  GAAGAG              
HapII          20  CCGG                 C^CGG
               74  CCGG                
HpaII          20  CCGG                 C^CGG
               74  CCGG                
Ksp632I       240  GAAGAG               CTCTTC(1/4)
              582  GAAGAG              
MflI          258  AGATCT               [GA]^GATC[CT]
              645  GGATCC              
MspA1I         34  CCGCTG               C[AC]G^C[GT]G
              777  CAGCGG              
MspI           20  CCGG                 C^CGG
               74  CCGG                
MvnI           12  CGCG                 CG^CG
              379  CGCG                
PleI          785  GACTC                GAGTC(4/5)
              909  GACTC               
PstI          683  CTGCAG               CTGCA^G
              956  CTGCAG              
SfcI          683  CTGCAG               C^T[GA][CT]AG
              956  CTGCAG              
TseI            7  GCAGC                G^C[AT]GC
              214  GCTGC               
Tsp509I       111  AATT                 ^AATT
              324  AATT                
XhoII         258  AGATCT               [GA]^GATC[CT]
              645  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   41 enzymes) #
#########################################

AciI        BsaJI       BsiYI       BslI        BsrI        
BssKI       BstF5I      BstNI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraII       EcoO109I    
EcoRII      Fnu4HI      FokI        HaeIII      HhaI        
HinP1I      HinfI       ItaI        MaeIII      MboI        
MboII       MnlI        MvaI        MwoI        NdeII       
NlaIII      NlaIV       Sau3AI      Sau96I      ScrFI       
SfaNI       TaqI        TfiI        Tsp45I      TspRI       
TthHB8I     
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