Restriction map for pF1KB6458
 (Linear) (Six-base) MAPSORT of: KIBB6458.seq  Check: 3884  from: 1  to: 1053



KIBB6458.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:47  ..

AccI GT'mk_AC

Cuts at:      0     224    1053 
   Size:        224     829
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     259     759     992    1053 
   Size:        259     500     233      61

  Fragments arranged by size: 

                 500     259     233      61
 
AhdI GACnn_n'nnGTC

Cuts at:      0     192    1053 
   Size:        192     861
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     775     785    1053 
   Size:        775      10     268

  Fragments arranged by size: 

                 775     268      10
 
ApaI G_GGCC'C

Cuts at:      0     721    1053 
   Size:        721     332
 
ApoI r'AATT_y

Cuts at:      0     323    1053 
   Size:        323     730
 
AvaI C'yCGr_G

Cuts at:      0     203    1053 
   Size:        203     850
 
BamHI G'GATC_C

Cuts at:      0     645    1053 
   Size:        645     408
 
BanII G_rGCy'C

Cuts at:      0     721    1053 
   Size:        721     332
 
BbvCI CC'TCA_GC

Cuts at:      0     317    1053 
   Size:        317     736
 
BglII A'GATC_T

Cuts at:      0     258    1053 
   Size:        258     795
 
Bme1580I G_kGCm'C

Cuts at:      0     721    1053 
   Size:        721     332
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     469     688    1053 
   Size:        469     219     365

  Fragments arranged by size: 

                 469     365     219
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     757    1053 
   Size:        757     296
 
Bpu10I CC'TnA_GC

Cuts at:      0     317    1053 
   Size:        317     736
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     433    1053 
   Size:        433     620
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     348    1053 
   Size:        348     705
 
BsaBI GATnn'nnATC

Cuts at:      0     644    1053 
   Size:        644     409
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     487     562    1053 
   Size:        487      75     491

  Fragments arranged by size: 

                 491     487      75
 
BseYI C'CCAG_C

Cuts at:      0     827    1053 
   Size:        827     226
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     200     929    1053 
   Size:        200     729     124

  Fragments arranged by size: 

                 729     200     124
 
Bsp1286I G_dGCh'C

Cuts at:      0     721    1053 
   Size:        721     332
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      49    1053 
   Size:         49    1004
 
BsrDI GCAATG_nn'

Cuts at:      0     394    1053 
   Size:        394     659
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     835    1053 
   Size:        835     218
 
BstYI r'GATC_y

Cuts at:      0     258     645    1053 
   Size:        258     387     408

  Fragments arranged by size: 

                 408     387     258
 
BtgI C'CryG_G

Cuts at:      0     338     843    1053 
   Size:        338     505     210

  Fragments arranged by size: 

                 505     338     210
 
BtsI GCAGTG_nn'

Cuts at:      0     509     692     935    1053 
   Size:        509     183     243     118

  Fragments arranged by size: 

                 509     243     183     118
 
ClaI AT'CG_AT

Cuts at:      0     332    1053 
   Size:        332     721
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     178     213    1053 
   Size:        178      35     840

  Fragments arranged by size: 

                 840     178      35
 
DraIII CAC_nnn'GTG

Cuts at:      0     814    1053 
   Size:        814     239
 
EaeI y'GGCC_r

Cuts at:      0    1018    1053 
   Size:       1018      35
 
EarI CTCTTCn'nnn_

Cuts at:      0     235     577    1053 
   Size:        235     342     476

  Fragments arranged by size: 

                 476     342     235
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     259     757     759     992    1053 
   Size:        259     498       2     233      61

  Fragments arranged by size: 

                 498     259     233      61       2
 
EcoO109I rG'GnC_Cy

Cuts at:      0     717     718     795    1053 
   Size:        717       1      77     258

  Fragments arranged by size: 

                 717     258      77       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     162    1053 
   Size:        162     891
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     178     210    1053 
   Size:        178      32     843

  Fragments arranged by size: 

                 843     178      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     281    1053 
   Size:        281     772
 
MscI TGG'CCA

Cuts at:      0    1020    1053 
   Size:       1020      33
 
MslI CAynn'nnrTG

Cuts at:      0      86    1053 
   Size:         86     967
 
MspA1I CmG'CkG

Cuts at:      0      36     779    1053 
   Size:         36     743     274

  Fragments arranged by size: 

                 743     274      36
 
NcoI C'CATG_G

Cuts at:      0     843    1053 
   Size:        843     210
 
NspI r_CATG'y

Cuts at:      0     762    1053 
   Size:        762     291
 
PasI CC'CwG_GG

Cuts at:      0     729    1053 
   Size:        729     324
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0       7      39    1053 
   Size:          7      32    1014

  Fragments arranged by size: 

                1014      32       7
 
PspOMI G'GGCC_C

Cuts at:      0     717    1053 
   Size:        717     336
 
PstI C_TGCA'G

Cuts at:      0     687     960    1053 
   Size:        687     273      93

  Fragments arranged by size: 

                 687     273      93
 
SfcI C'TryA_G

Cuts at:      0     683     956    1053 
   Size:        683     273      97

  Fragments arranged by size: 

                 683     273      97
 
SmlI C'TyrA_G

Cuts at:      0     448    1053 
   Size:        448     605
 
SphI G_CATG'C

Cuts at:      0     762    1053 
   Size:        762     291
 
StyI C'CwwG_G

Cuts at:      0     843    1053 
   Size:        843     210
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     418    1053 
   Size:        418     635
 
TatI w'GTAC_w

Cuts at:      0     268    1053 
   Size:        268     785
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     824     856    1053 
   Size:        824      32     197

  Fragments arranged by size: 

                 824     197      32
 

 Enzymes that do cut:

     AccI     AcuI     AhdI     AlfI     ApaI     ApoI     AvaI    BamHI
    BanII    BbvCI    BglII Bme1580I     BmrI     BpmI   Bpu10I    BpuEI
     BsaI    BsaBI    BseRI    BseYI     BsgI Bsp1286I    BspMI    BsrDI
    BstXI    BstYI     BtgI     BtsI     ClaI    CspCI   DraIII     EaeI
     EarI  Eco57MI EcoO109I     FalI    Hin4I     MmeI     MscI     MslI
   MspA1I     NcoI     NspI     PasI     PpiI   PspOMI     PstI     SfcI
     SmlI     SphI     StyI    TaqII     TatI     TstI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AleI     AloI    AlwNI    ApaLI     AscI     AseI    AsiSI    AvrII
     BaeI     BanI     BbeI     BbsI     BcgI    BciVI     BclI    BfrBI
     BglI     BlpI    BmgBI     BmtI     BplI    BsaAI    BsaHI    BsaWI
    BsaXI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
  BstZ17I   Bsu36I    BtgZI     DraI     DrdI     EagI     EciI  EcoICRI
    EcoNI    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     NaeI     NarI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PciI
    PflMI     PfoI     PmeI     PmlI    PpuMI    PshAI     PsiI    PspXI
     PsrI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SrfI     SspI     StuI     SwaI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI
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