Restriction map for pF1KE0172
##################################
# NO CUT ENZYMES (  139 enzymes) #
##################################

Acc65I      AciI        AclI        AflIII      AgeI        
AhdI        Alw44I      AocI        Aor51HI     ApaLI       
AscI        AseI        AsnI        Asp718I     AspEI       
AvaI        AviII       AvrII       BamHI       BbeI        
BbrPI       BcgI        BciVI       BclI        BcnI        
BglI        BglII       BlnI        BlpI        Bpu1102I    
BsaAI       BsaI        BsiEI       BsiYI       BslI        
BsoBI       Bsp1407I    BspDI       BspHI       BspLU11I    
BspMI       BsrBI       BsrDI       BsrFI       BsrGI       
BssHII      Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstZ17I     Bsu36I      CelII       Cfr10I      
ClaI        CpoI        DraI        DraIII      DrdI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO65I     EcoRI       EcoT22I     
FauI        FbaI        FseI        FspI        HaeII       
HgaI        HindIII     HpaI        KasI        KpnI        
KspI        MfeI        MluI        MspA1I      MunI        
MwoI        NaeI        NarI        NciI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflMI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PvuI        
PvuII       RcaI        RsrII       SacI        SacII       
SalI        SbfI        ScaI        SexAI       SfaNI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        Van91I      XbaI        
XcmI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   77 enzymes) #
###################################

AatII         837  GACGTC               GACGT^C
AccI          595  GTAGAC               GT^[AC][GT]AC
AccII         250  CGCG                 CG^CG
AccIII        443  TCCGGA               T^CCGGA
AcsI          133  GAATTT               [GA]^AATT[CT]
AcyI          837  GACGTC               G[GA]^CG[CT]C
AfaI          126  GTAC                 GT^AC
AflII         435  CTTAAG               C^TTAAG
AlwNI         509  CAGGGACTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI          120  GGGCCC               GGGCC^C
ApoI          133  GAATTT               [GA]^AATT[CT]
Asp700I       108  GAAGAGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI         225  GTGCTC               G[AT]GC[AT]^C
AspI          598  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaII         351  GGACC                G^G[AT]CC
BalI           71  TGGCCA               TGG^CCA
BanI          810  GGCACC               G^G[CT][GA]CC
BbsI          298  GTCTTC               GAAGAC(2/6)
BbvCI         415  CCTCAGC              CCTCAGC(-5/-2)
BbvI            5  GCTGC                GCAGC(8/12)
BfaI          890  CTAG                 C^TAG
BfrI          435  CTTAAG               C^TTAAG
BpmI          475  CTGGAG               CTGGAG(16/14)
BpuAI         298  GTCTTC               GAAGAC(2/6)
BsaBI         555  GATCCGTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI         837  GACGTC               G[GA]^CG[CT]C
BsaWI         443  TCCGGA               [AT]^CCGG[AT]
BseAI         443  TCCGGA               T^CCGGA
BsiHKAI       225  GTGCTC               G[AT]GC[AT]^C
BsiWI         125  CGTACG               C^GTACG
BsmBI         839  CGTCTC               CGTCTC(1/5)
BsmI          826  GCATTC               GAATGC(1/-1)
BspEI         443  TCCGGA               T^CCGGA
BsrI          218  ACTGG                ACTGG(1/-1)
BssSI         385  CACGAG               CACGAG(-5/-1)
BstF5I         66  GGATG                GGATG(2/0)
BstUI         250  CGCG                 CG^CG
BstXI         714  CCATGCTAATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I         885  GCTGGC               GC[ACGT]^[ACGT]GC
CfoI          249  GCGC                 GCG^C
DraII         272  AGGGCCT              [GA]G^G[ACGT]CC[CT]
DsaI           62  CCGTGG               C^C[GA][CT]GG
EaeI           71  TGGCCA               [CT]^GGCC[GA]
EcoO109I      272  AGGGCCT              [GA]G^G[ACGT]CC[CT]
EcoRV         361  GATATC               GAT^ATC
EcoT14I       269  CCAAGG               C^C[AT][AT]GG
Fnu4HI          5  GCTGC                GC^[ACGT]GC
FokI           66  GGATG                GGATG(9/13)
HapII         444  CCGG                 C^CGG
HhaI          249  GCGC                 GCG^C
Hin1I         837  GACGTC               G[GA]^CG[CT]C
HinP1I        249  GCGC                 G^CGC
HincII        861  GTCAAC               GT[CT]^[GA]AC
HindII        861  GTCAAC               GT[CT]^[GA]AC
HpaII         444  CCGG                 C^CGG
HphI           97  TCACC                GGTGA(8/7)
ItaI            5  GCTGC                GC^[ACGT]GC
MaeI          890  CTAG                 C^TAG
MaeIII        505  GTGAC                ^GT[ACGT]AC
MamI          555  GATCCGTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI          71  TGGCCA               TGG^CCA
MroI          443  TCCGGA               T^CCGGA
MscI           71  TGGCCA               TGG^CCA
MspI          444  CCGG                 C^CGG
MvnI          250  CGCG                 CG^CG
PflFI         598  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PstI          334  CTGCAG               CTGCA^G
RsaI          126  GTAC                 GT^AC
SapI          897  GCTCTTC              GCTCTTC(1/4)
SfcI          334  CTGCAG               C^T[GA][CT]AG
SmlI          435  CTTAAG               C^T[CT][GA]AG
StyI          269  CCAAGG               C^C[AT][AT]GG
TfiI          571  GATTC                G^A[AT]TC
TseI            5  GCTGC                G^C[AT]GC
Tsp45I        505  GTGAC                ^GT[GC]AC
Tth111I       598  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XmnI          108  GAAGAGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   11 enzymes) #
###################################

AluI            4  AGCT                 AG^CT
              916  AGCT                
BanII         120  GGGCCC               G[GA]GC[CT]^C
              895  GGGCTC              
BseRI         366  CTCCTC               GAGGAG(10/8)
              388  GAGGAG              
BsgI            6  CTGCAC               GTGCAG(16/14)
              796  GTGCAG              
BsmAI         182  GTCTC                GTCTC(1/5)
              840  GTCTC               
BsmFI         511  GGGAC                GGGAC(10/14)
              737  GGGAC               
EarI          898  CTCTTC               CTCTTC(1/4)
              108  GAAGAG              
Ksp632I       898  CTCTTC               CTCTTC(1/4)
              108  GAAGAG              
PleI          265  GACTC                GAGTC(4/5)
              859  GAGTC               
TaqI          569  TCGA                 T^CGA
              948  TCGA                
TthHB8I       569  TCGA                 T^CGA
              948  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   31 enzymes) #
#########################################

AlwI        BmyI        BsaJI       Bsp1286I    BssKI       
BstNI       BstYI       Cfr13I      DdeI        DpnII       
EcoRII      HaeIII      HinfI       MaeII       MboI        
MboII       MflI        MnlI        MseI        MslI        
MvaI        NdeII       NlaIII      NlaIV       Sau3AI      
Sau96I      ScrFI       Tru9I       Tsp509I     TspRI       
XhoII       
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