Restriction map for pF1KB6426
##################################
# NO CUT ENZYMES (  141 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AflIII      AgeI        Alw44I      
AocI        Aor51HI     ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp718I     AspI        
AviII       AvrII       BamHI       BanII       BbrPI       
BbvCI       BciVI       BclI        BfaI        BfrI        
BlnI        BlpI        Bpu1102I    BsaAI       BsaBI       
BsaWI       BseAI       BseRI       BsiWI       BsmI        
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrBI       BsrDI       BsrFI       BsrGI       BssHII      
Bst1107I    BstAPI      BstBI       BstEII      BstF5I      
BstPI       BstXI       BstZ17I     Bsu36I      CelII       
Cfr10I      ClaI        CpoI        DraI        EagI        
EarI        EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO65I     EcoRI       EcoRV       EcoT22I     
FbaI        FokI        FseI        FspI        HincII      
HindII      HindIII     HpaI        KpnI        Ksp632I     
KspI        MaeI        MamI        MfeI        MluI        
MroI        MseI        MunI        NaeI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        SfiI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        SwaI        Tru9I       
Tth111I     Van91I      XbaI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AccII         351  CGCG                 CG^CG
AhdI          403  GACCTGCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI         460  CAGGGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
AspEI         403  GACCTGCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI          563  TGGCCA               TGG^CCA
BbsI          622  GAAGAC               GAAGAC(2/6)
BcgI          633  CGAGTTCTTTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI          399  CCCGG                CC^[GC]GG
BglI          383  GCCACGGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         371  AGATCT               A^GATCT
BpmI          809  CTGGAG               CTGGAG(16/14)
BpuAI         622  GAAGAC               GAAGAC(2/6)
BsgI          505  GTGCAG               GTGCAG(16/14)
BsiEI         525  CGACCG               CG[GA][CT]^CG
BsrI           69  CCAGT                ACTGG(1/-1)
BssSI         241  CACGAG               CACGAG(-5/-1)
BstUI         351  CGCG                 CG^CG
DdeI          669  CTCAG                C^T[ACGT]AG
DraII         461  AGGGCCT              [GA]G^G[ACGT]CC[CT]
DraIII        610  CACCAGGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI          526  GACCGTGGCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I      403  GACCTGCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I      461  AGGGCCT              [GA]G^G[ACGT]CC[CT]
EcoT14I       167  CCTTGG               C^C[AT][AT]GG
HapII         400  CCGG                 C^CGG
HpaII         400  CCGG                 C^CGG
MaeII         503  ACGT                 A^CGT
MluNI         563  TGGCCA               TGG^CCA
MscI          563  TGGCCA               TGG^CCA
MspA1I        652  CAGCTG               C[AC]G^C[GT]G
MspI          400  CCGG                 C^CGG
MvnI          351  CGCG                 CG^CG
NciI          399  CCCGG                CC^[GC]GG
PleI           28  GACTC                GAGTC(4/5)
PstI          406  CTGCAG               CTGCA^G
PvuII         652  CAGCTG               CAG^CTG
ScaI           71  AGTACT               AGT^ACT
SexAI         611  ACCAGGT              A^CC[AT]GGT
SfcI          406  CTGCAG               C^T[GA][CT]AG
SmlI          754  CTCAAG               C^T[CT][GA]AG
StuI          390  AGGCCT               AGG^CCT
StyI          167  CCTTGG               C^C[AT][AT]GG
TaqI           31  TCGA                 T^CGA
TfiI          423  GATTC                G^A[AT]TC
Tsp509I       838  AATT                 ^AATT
TthHB8I        31  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   33 enzymes) #
###################################

AlwI          750  GATCC                GGATC(4/5)
              431  GGATC               
Asp700I       216  GAACTTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              315  GAAAACCTTC          
AspHI         605  GAGCAC               G[AT]GC[AT]^C
              738  GTGCTC              
AvaI          115  CCCGAG               C^[CT]CG[GA]G
              211  CCCGAG              
AvaII         402  GGACC                G^G[AT]CC
              591  GGACC               
BanI           41  GGCGCC               G^G[CT][GA]CC
              706  GGCGCC              
BbeI           41  GGCGCC               GGCGC^C
              706  GGCGCC              
BmyI          605  GAGCAC               G[AGT]GC[ACT]^C
              738  GTGCTC              
BsaI          172  GAGACC               GGTCTC(1/5)
              727  GAGACC              
BsiHKAI       605  GAGCAC               G[AT]GC[AT]^C
              738  GTGCTC              
BsmBI         534  CGTCTC               CGTCTC(1/5)
              291  GAGACG              
BsmFI         140  GGGAC                GGGAC(10/14)
              396  GTCCC               
BsoBI         115  CCCGAG               C^[CT]CG[GA]G
              211  CCCGAG              
Bsp1286I      605  GAGCAC               G[AGT]GC[ACT]^C
              738  GTGCTC              
BspMI         404  ACCTGC               ACCTGC(4/8)
              476  ACCTGC              
BstYI         371  AGATCT               [GA]^GATC[CT]
              749  AGATCC              
CfoI           42  GCGC                 GCG^C
              707  GCGC                
FauI          760  CCCGC                CCCGC(4/6)
              833  CCCGC               
HaeII          41  GGCGCC               [GA]GCGC^[CT]
              706  GGCGCC              
HgaI          293  GACGC                GACGC(5/10)
              533  GCGTC               
HhaI           42  GCGC                 GCG^C
              707  GCGC                
HinP1I         42  GCGC                 G^CGC
              707  GCGC                
HinfI          28  GACTC                G^A[ACGT]TC
              423  GATTC               
KasI           41  GGCGCC               G^GCGCC
              706  GGCGCC              
MflI          371  AGATCT               [GA]^GATC[CT]
              749  AGATCC              
MslI          241  CACGAGTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              444  CACAGATGTG          
NarI           41  GGCGCC               GG^CGCC
              706  GGCGCC              
SfaNI         254  GCATC                GCATC(5/9)
              263  GCATC               
SfoI           41  GGCGCC               GGC^GCC
              706  GGCGCC              
TspRI         324  CACTG                CA[GC]TG[ACGT][ACGT]^
              795  CAGTG               
XcmI          644  CCAAGCGGCAGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              710  CCATGATGAGTGTGG     
XhoII         371  AGATCT               [GA]^GATC[CT]
              749  AGATCC              
XmnI          216  GAACTTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              315  GAAAACCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   38 enzymes) #
#########################################

AciI        AcyI        AfaI        AluI        BbvI        
BsaHI       BsaJI       BsiYI       BslI        BsmAI       
BssKI       BstNI       Cac8I       Cfr13I      DpnII       
DsaI        EaeI        EcoRII      Fnu4HI      HaeIII      
Hin1I       HphI        ItaI        MaeIII      MboI        
MboII       MnlI        MvaI        MwoI        NdeII       
NlaIII      NlaIV       RsaI        Sau3AI      Sau96I      
ScrFI       TseI        Tsp45I      
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