Restriction map for pF1KB8177
 (Linear) (Six-base) MAPSORT of: KIBB8177.seq  Check: 5091  from: 1  to: 1602



KIBB8177.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:50  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     534    1602 
   Size:        534    1068
 
AccI GT'mk_AC

Cuts at:      0    1377    1602 
   Size:       1377     225
 
Acc65I G'GTAC_C

Cuts at:      0     486    1602 
   Size:        486    1116
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     446     864    1602 
   Size:        446     418     738

  Fragments arranged by size: 

                 738     446     418
 
AflIII A'CryG_T

Cuts at:      0     557    1602 
   Size:        557    1045
 
AleI CACnn'nnGTG

Cuts at:      0     206     442     737    1602 
   Size:        206     236     295     865

  Fragments arranged by size: 

                 865     295     236     206
 
AlwNI CAG_nnn'CTG

Cuts at:      0     511    1480    1602 
   Size:        511     969     122

  Fragments arranged by size: 

                 969     511     122
 
ApaLI G'TGCA_C

Cuts at:      0     199    1584    1602 
   Size:        199    1385      18

  Fragments arranged by size: 

                1385     199      18
 
ApoI r'AATT_y

Cuts at:      0     501    1203    1223    1602 
   Size:        501     702      20     379

  Fragments arranged by size: 

                 702     501     379      20
 
AseI AT'TA_AT

Cuts at:      0     545    1602 
   Size:        545    1057
 
BanI G'GyrC_C

Cuts at:      0     486    1602 
   Size:        486    1116
 
BanII G_rGCy'C

Cuts at:      0     638     680    1602 
   Size:        638      42     922

  Fragments arranged by size: 

                 922     638      42
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     857    1148    1602 
   Size:        857     291     454

  Fragments arranged by size: 

                 857     454     291
 
BclI T'GATC_A

Cuts at:      0     924    1469    1602 
   Size:        924     545     133

  Fragments arranged by size: 

                 924     545     133
 
BglII A'GATC_T

Cuts at:      0     875    1602 
   Size:        875     727
 
Bme1580I G_kGCm'C

Cuts at:      0     203    1588    1602 
   Size:        203    1385      14

  Fragments arranged by size: 

                1385     203      14
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     225    1602 
   Size:        225    1377
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     326    1069    1201    1588    1602 
   Size:        326     743     132     387      14

  Fragments arranged by size: 

                 743     387     326     132      14
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     385     642    1082    1492    1602 
   Size:        385     257     440     410     110

  Fragments arranged by size: 

                 440     410     385     257     110
 
BsaBI GATnn'nnATC

Cuts at:      0     929    1602 
   Size:        929     673
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      44      74     316     346    1602 
   Size:         44      30     242      30    1256

  Fragments arranged by size: 

                1256     242      44      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     337     618    1477    1602 
   Size:        337     281     859     125

  Fragments arranged by size: 

                 859     337     281     125
 
BseYI C'CCAG_C

Cuts at:      0     628     854    1602 
   Size:        628     226     748

  Fragments arranged by size: 

                 748     628     226
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     255    1454    1602 
   Size:        255    1199     148

  Fragments arranged by size: 

                1199     255     148
 
BsiHKAI G_wGCw'C

Cuts at:      0     203     846    1588    1602 
   Size:        203     643     742      14

  Fragments arranged by size: 

                 742     643     203      14
 
BsmI GAATG_Cn'

Cuts at:      0     257    1602 
   Size:        257    1345
 
Bsp1286I G_dGCh'C

Cuts at:      0     203     638     680     846    1588    1602 
   Size:        203     435      42     166     742      14

  Fragments arranged by size: 

                 742     435     203     166      42      14
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     534    1602 
   Size:        534    1068
 
BsrDI GCAATG_nn'

Cuts at:      0    1157    1409    1602 
   Size:       1157     252     193

  Fragments arranged by size: 

                1157     252     193
 
BsrFI r'CCGG_y

Cuts at:      0      91     380    1602 
   Size:         91     289    1222

  Fragments arranged by size: 

                1222     289      91
 
BsrGI T'GTAC_A

Cuts at:      0     293     468    1602 
   Size:        293     175    1134

  Fragments arranged by size: 

                1134     293     175
 
BstYI r'GATC_y

Cuts at:      0     875     883    1538    1602 
   Size:        875       8     655      64

  Fragments arranged by size: 

                 875     655      64       8
 
BtgI C'CryG_G

Cuts at:      0     395     413    1602 
   Size:        395      18    1189

  Fragments arranged by size: 

                1189     395      18
 
EarI CTCTTCn'nnn_

Cuts at:      0    1588    1602 
   Size:       1588      14
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     326     446     864    1069    1201    1588    1602 
   Size:        326     120     418     205     132     387      14

  Fragments arranged by size: 

                 418     387     326     205     132     120      14
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1015    1602 
   Size:       1015     587
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1256    1602 
   Size:       1256     346
 
EcoRI G'AATT_C

Cuts at:      0     501    1602 
   Size:        501    1101
 
EcoRV GAT'ATC

Cuts at:      0     262    1602 
   Size:        262    1340
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1461    1493    1602 
   Size:       1461      32     109

  Fragments arranged by size: 

                1461     109      32
 
HincII GTy'rAC

Cuts at:      0    1518    1602 
   Size:       1518      84
 
KpnI G_GTAC'C

Cuts at:      0     490    1602 
   Size:        490    1112
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     198     948    1505    1602 
   Size:        198     750     557      97

  Fragments arranged by size: 

                 750     557     198      97
 
MslI CAynn'nnrTG

Cuts at:      0     206     442     737    1602 
   Size:        206     236     295     865

  Fragments arranged by size: 

                 865     295     236     206
 
MspA1I CmG'CkG

Cuts at:      0     358    1291    1602 
   Size:        358     933     311

  Fragments arranged by size: 

                 933     358     311
 
NcoI C'CATG_G

Cuts at:      0     395     413    1602 
   Size:        395      18    1189

  Fragments arranged by size: 

                1189     395      18
 
NspI r_CATG'y

Cuts at:      0     561    1602 
   Size:        561    1041
 
PasI CC'CwG_GG

Cuts at:      0     511    1602 
   Size:        511    1091
 
PciI A'CATG_T

Cuts at:      0     557    1602 
   Size:        557    1045
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     691    1602 
   Size:        691     911
 
PfoI T'CCnGG_A

Cuts at:      0    1217    1252    1602 
   Size:       1217      35     350

  Fragments arranged by size: 

                1217     350      35
 
PpuMI rG'GwC_Cy

Cuts at:      0    1256    1602 
   Size:       1256     346
 
PvuII CAG'CTG

Cuts at:      0     358    1291    1602 
   Size:        358     933     311

  Fragments arranged by size: 

                 933     358     311
 
SexAI A'CCwGG_T

Cuts at:      0     182     563    1602 
   Size:        182     381    1039

  Fragments arranged by size: 

                1039     381     182
 
SfcI C'TryA_G

Cuts at:      0     662    1602 
   Size:        662     940
 
SmlI C'TyrA_G

Cuts at:      0       5    1602 
   Size:          5    1597
 
SspI AAT'ATT

Cuts at:      0    1094    1602 
   Size:       1094     508
 
StyI C'CwwG_G

Cuts at:      0     395     413     638    1602 
   Size:        395      18     225     964

  Fragments arranged by size: 

                 964     395     225      18
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     868    1602 
   Size:        868     734
 
TatI w'GTAC_w

Cuts at:      0     293     468    1602 
   Size:        293     175    1134

  Fragments arranged by size: 

                1134     293     175
 
Tth111I GACn'n_nGTC

Cuts at:      0     351    1602 
   Size:        351    1251
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1044    1563    1602 
   Size:       1044     519      39

  Fragments arranged by size: 

                1044     519      39
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AcuI   AflIII     AleI    AlwNI    ApaLI
     ApoI     AseI     BanI    BanII     BbsI     BclI    BglII Bme1580I
     BmrI     BpmI     BsaI    BsaBI    BsaXI    BseRI    BseYI     BsgI
  BsiHKAI     BsmI Bsp1286I    BspMI    BsrDI    BsrFI    BsrGI    BstYI
     BtgI     EarI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV    Hin4I
   HincII     KpnI     MmeI     MslI   MspA1I     NcoI     NspI     PasI
     PciI    PflMI     PfoI    PpuMI    PvuII    SexAI     SfcI     SmlI
     SspI     StyI    TaqII     TatI  Tth111I     XcmI

 Enzymes that do not cut: 

    AatII     AclI     AfeI    AflII     AgeI     AhdI     AlfI     AloI
     ApaI     AscI    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI
    BbvCI     BcgI    BciVI    BfrBI     BglI     BlpI    BmgBI     BmtI
     BplI   Bpu10I    BpuEI    BsaAI    BsaHI    BsaWI    BsiEI    BsiWI
    BsmBI    BspEI    BspHI    BsrBI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI     FalI
     FseI     FspI    FspAI    HaeII  HindIII     HpaI     KasI     MfeI
     MluI     MscI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PmeI     PmlI     PpiI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     StuI     SwaI
     TstI     XbaI     XhoI     XmaI     XmnI     ZraI
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