Restriction map for
pF1KB6406
(Linear) (Six-base) MAPSORT of: KIBB6406.seq Check: 1367 from: 1 to: 1005
KIBB6406.orf
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 191 587 1005
Size: 191 396 418
Fragments arranged by size:
418 396 191
AlwNI CAG_nnn'CTG
Cuts at: 0 130 1005
Size: 130 875
ApoI r'AATT_y
Cuts at: 0 446 1005
Size: 446 559
AvaI C'yCGr_G
Cuts at: 0 302 1005
Size: 302 703
BanI G'GyrC_C
Cuts at: 0 15 1005
Size: 15 990
BanII G_rGCy'C
Cuts at: 0 852 1005
Size: 852 153
BbeI G_GCGC'C
Cuts at: 0 19 1005
Size: 19 986
BglII A'GATC_T
Cuts at: 0 369 1005
Size: 369 636
BmrI ACTGGGnnnn_n'
Cuts at: 0 719 827 1005
Size: 719 108 178
Fragments arranged by size:
719 178 108
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 209 932 1005
Size: 209 723 73
Fragments arranged by size:
723 209 73
BsaAI yAC'GTr
Cuts at: 0 247 1005
Size: 247 758
BsaHI Gr'CG_yC
Cuts at: 0 16 1005
Size: 16 989
BsaWI w'CCGG_w
Cuts at: 0 995 1005
Size: 995 10
BseYI C'CCAG_C
Cuts at: 0 592 1005
Size: 592 413
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 572 1005
Size: 572 433
Bsp1286I G_dGCh'C
Cuts at: 0 852 1005
Size: 852 153
BsrDI GCAATG_nn'
Cuts at: 0 518 1005
Size: 518 487
BssSI C'ACGA_G
Cuts at: 0 452 1005
Size: 452 553
BstYI r'GATC_y
Cuts at: 0 369 1005
Size: 369 636
EarI CTCTTCn'nnn_
Cuts at: 0 452 540 1005
Size: 452 88 465
Fragments arranged by size:
465 452 88
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 191 209 587 932 1005
Size: 191 18 378 345 73
Fragments arranged by size:
378 345 191 73 18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 191 685 862 1005
Size: 191 494 177 143
Fragments arranged by size:
494 191 177 143
EcoRI G'AATT_C
Cuts at: 0 446 1005
Size: 446 559
EcoRV GAT'ATC
Cuts at: 0 768 1005
Size: 768 237
HaeII r_GCGC'y
Cuts at: 0 19 1005
Size: 19 986
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 667 699 1005
Size: 667 32 306
Fragments arranged by size:
667 306 32
KasI G'GCGC_C
Cuts at: 0 15 1005
Size: 15 990
MslI CAynn'nnrTG
Cuts at: 0 49 1005
Size: 49 956
NarI GG'CG_CC
Cuts at: 0 16 1005
Size: 16 989
NspI r_CATG'y
Cuts at: 0 54 1005
Size: 54 951
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 685 717 1005
Size: 685 32 288
Fragments arranged by size:
685 288 32
PspXI vC'TCGA_Gb
Cuts at: 0 302 1005
Size: 302 703
SfcI C'TryA_G
Cuts at: 0 524 1005
Size: 524 481
SfoI GGC'GCC
Cuts at: 0 17 1005
Size: 17 988
SmlI C'TyrA_G
Cuts at: 0 302 1005
Size: 302 703
SnaBI TAC'GTA
Cuts at: 0 247 1005
Size: 247 758
SspI AAT'ATT
Cuts at: 0 424 1005
Size: 424 581
StyI C'CwwG_G
Cuts at: 0 899 989 1005
Size: 899 90 16
Fragments arranged by size:
899 90 16
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 697 1005
Size: 697 308
TatI w'GTAC_w
Cuts at: 0 104 483 884 1005
Size: 104 379 401 121
Fragments arranged by size:
401 379 121 104
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 879 911 1005
Size: 879 32 94
Fragments arranged by size:
879 94 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 567 897 1005
Size: 567 330 108
Fragments arranged by size:
567 330 108
XhoI C'TCGA_G
Cuts at: 0 302 1005
Size: 302 703
XmnI GAAnn'nnTTC
Cuts at: 0 260 1005
Size: 260 745
Enzymes that do cut:
AcuI AlwNI ApoI AvaI BanI BanII BbeI BglII
BmrI BpmI BsaAI BsaHI BsaWI BseYI BsgI Bsp1286I
BsrDI BssSI BstYI EarI Eco57MI EcoNI EcoRI EcoRV
HaeII Hin4I KasI MslI NarI NspI PpiI PspXI
SfcI SfoI SmlI SnaBI SspI StyI TaqII TatI
TstI XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI
AseI AsiSI AvrII BaeI BamHI BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BlpI Bme1580I BmgBI BmtI
BplI Bpu10I BpuEI BsaI BsaBI BsaXI BseRI BsiEI
BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI
BsrFI BsrGI BssHII BstAPI BstBI BstEII BstXI BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EciI EcoICRI EcoO109I FalI FseI
FspI FspAI HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PciI PflMI PfoI
PmeI PmlI PpuMI PshAI PsiI PspOMI PsrI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SpeI
SphI SrfI StuI SwaI Tth111I XbaI XmaI ZraI