Restriction map for pF1KB5985
##################################
# NO CUT ENZYMES (   97 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AhdI        Alw44I      ApaLI       
ApoI        AseI        AsnI        Asp718I     AspEI       
AviII       AvrII       BamHI       BbeI        BbsI        
BbvCI       BcgI        BciVI       BclI        BfrI        
BglII       BlnI        BpuAI       BseAI       BsgI        
BsiWI       BsmI        Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrDI       BsrGI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstXI       BstZ17I     ClaI        DraI        DrdI        
Eam1105I    EcoO65I     EcoRI       EcoRV       EcoT22I     
FbaI        FseI        FspI        HindIII     KasI        
KpnI        MfeI        MluI        MroI        MunI        
NarI        NcoI        NdeI        NheI        NotI        
NruI        NsiI        NspV        PacI        PmeI        
PshAI       PshBI       Psp1406I    RcaI        SalI        
SapI        SbfI        ScaI        SexAI       SfoI        
SfuI        SgrAI       SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
Tsp509I     XbaI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AflIII       1222  ACGTGT               A^C[GA][CT]GT
AgeI         1382  ACCGGT               A^CCGGT
AocI          970  CCTGAGG              CC^T[ACGT]AGG
Aor51HI      1208  AGCGCT               AGC^GCT
AscI         1368  GGCGCGCC             GG^CGCGCC
AspI         1426  GACATGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BanI          361  GGCACC               G^G[CT][GA]CC
BglI          883  GCCAACTGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI          397  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      397  GCTGAGC              GC^T[ACGT]AGC
BsaBI         657  GATGAGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI         863  GAGACG               CGTCTC(1/5)
BsrBI        1041  CCGCTC               CCGCTC(-3/-3)
BssHII       1369  GCGCGC               G^CGCGC
BssSI        1156  CTCGTG               CACGAG(-5/-1)
BstF5I       1491  CATCC                GGATG(2/0)
Bsu36I        970  CCTGAGG              CC^T[ACGT]AGG
CelII         397  GCTGAGC              GC^T[ACGT]AGC
CpoI          125  CGGACCG              CG^G[AT]CCG
DraIII       1153  CACCTCGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI         1319  CCGCGG               C^C[GA][CT]GG
EagI         1163  CGGCCG               C^GGCCG
EclXI        1163  CGGCCG               C^GGCCG
Eco47III     1208  AGCGCT               AGC^GCT
Eco52I       1163  CGGCCG               C^GGCCG
Eco81I        970  CCTGAGG              CC^T[ACGT]AGG
EcoNI         111  CCTGACCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FokI         1491  CATCC                GGATG(9/13)
HincII        205  GTTAAC               GT[CT]^[GA]AC
HindII        205  GTTAAC               GT[CT]^[GA]AC
HpaI          205  GTTAAC               GTT^AAC
KspI         1319  CCGCGG               CCGC^GG
MamI          657  GATGAGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI          206  TTAA                 T^TAA
NaeI         1000  GCCGGC               GCC^GGC
NgoMIV       1000  GCCGGC               G^CCGGC
NspI          791  GCATGT               [GA]CATG^[CT]
PaeR7I         34  CTCGAG               C^TCGAG
PflFI        1426  GACATGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PinAI        1382  ACCGGT               A^CCGGT
PstI          805  CTGCAG               CTGCA^G
PvuI          927  CGATCG               CGAT^CG
RsrII         125  CGGACCG              CG^G[AT]CCG
SacI         1009  GAGCTC               GAGCT^C
SacII        1319  CCGCGG               CCGC^GG
SfiI          882  GGCCAACTGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Tru9I         206  TTAA                 T^TAA
Tth111I      1426  GACATGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoI           34  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

ApaI          890  GGGCCC               GGGCC^C
             1073  GGGCCC              
Asp700I       909  GAAAGACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1449  GAAGCAGTTC          
AspHI         135  GTGCTC               G[AT]GC[AT]^C
             1009  GAGCTC              
BalI          752  TGGCCA               TGG^CCA
              991  TGGCCA              
BbrPI        1177  CACGTG               CAC^GTG
             1221  CACGTG              
BfaI          460  CTAG                 C^TAG
             1195  CTAG                
BsaAI        1177  CACGTG               [CT]AC^GT[GA]
             1221  CACGTG              
BsaI          172  GAGACC               GGTCTC(1/5)
              322  GAGACC              
BsiEI         927  CGATCG               CG[GA][CT]^CG
             1163  CGGCCG              
BsiHKAI       135  GTGCTC               G[AT]GC[AT]^C
             1009  GAGCTC              
EcoT14I       701  CCAAGG               C^C[AT][AT]GG
              948  CCTTGG              
MaeI          460  CTAG                 C^TAG
             1195  CTAG                
MluNI         752  TGGCCA               TGG^CCA
              991  TGGCCA              
MscI          752  TGGCCA               TGG^CCA
              991  TGGCCA              
MslI          852  CATCTTTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1428  CATGGTCATG          
PflMI         566  CCAGTGACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              741  CCAGGTGCTGG         
PleI         1402  GACTC                GAGTC(4/5)
             1066  GAGTC               
PmaCI        1177  CACGTG               CAC^GTG
             1221  CACGTG              
PmlI         1177  CACGTG               CAC^GTG
             1221  CACGTG              
PvuII         196  CAGCTG               CAG^CTG
              473  CAGCTG              
SfcI          805  CTGCAG               C^T[GA][CT]AG
             1443  CTACAG              
SmaI           18  CCCGGG               CCC^GGG
              894  CCCGGG              
StyI          701  CCAAGG               C^C[AT][AT]GG
              948  CCTTGG              
Van91I        566  CCAGTGACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              741  CCAGGTGCTGG         
XcmI          140  CCAGAACTCAAGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1088  CCAGCCCGGAGGTGG     
XmaCI          18  CCCGGG               C^CCGGG
              894  CCCGGG              
XmaI           18  CCCGGG               C^CCGGG
              894  CCCGGG              
XmnI          909  GAAAGACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1449  GAAGCAGTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   84 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AlwNI       AvaI        AvaII       BanII       
BbvI        BcnI        BmyI        BpmI        BsaHI       
BsaJI       BsaWI       BseRI       BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    BsrFI       
BsrI        BssKI       BstNI       BstUI       BstYI       
Cac8I       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       EaeI        EarI        EcoO109I    
EcoRII      FauI        Fnu4HI      HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeII       MaeIII      MboI        MboII       MflI        
MnlI        MspA1I      MspI        MvaI        MvnI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
PpuMI       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SmlI        TaqI        TfiI        TseI        
Tsp45I      TspRI       TthHB8I     XhoII       
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