Restriction map for pF1KB5985
 (Circular) (Six-base) MAPSORT of: pF1KB5985.seq  Check: 1712  from: 1  to: 4676



pF1KB5985  4676 bp

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:45  ..

AccI GT'mk_AC

Cuts at:   1714    1714 
   Size:       4676
 
Acc65I G'GTAC_C

Cuts at:   1692    1692 
   Size:       4676
 
AclI AA'CG_TT

Cuts at:   4516    4516 
   Size:       4676
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1366    1604    2501    2933    3680    3948    1366 
   Size:        238     897     432     747     268    2094

  Fragments arranged by size: 

                2094     897     747     432     268     238
 
AfeI AGC'GCT

Cuts at:   1318    1318 
   Size:       4676
 
AflIII A'CryG_T

Cuts at:   1330    2028    3132    4109    1330 
   Size:        698    1104     977    1897

  Fragments arranged by size: 

                1897    1104     977     698
 
AgeI A'CCGG_T

Cuts at:   1490    3064    1490 
   Size:       1574    3102
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   1597    1629    1597 
   Size:         32    4644
 
AlwNI CAG_nnn'CTG

Cuts at:    277     680     855    1981    3548     277 
   Size:        403     175    1126    1567    1405

  Fragments arranged by size: 

                1567    1405    1126     403     175
 
ApaI G_GGCC'C

Cuts at:   1002    1185    1002 
   Size:        183    4493
 
ApaLI G'TGCA_C

Cuts at:   3446    3446 
   Size:       4676
 
ApoI r'AATT_y

Cuts at:   1680    4029    4285    1680 
   Size:       2349     256    2071

  Fragments arranged by size: 

                2349    2071     256
 
AscI GG'CGCG_CC

Cuts at:   1477    1477 
   Size:       4676
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4676
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4676
 
AvaI C'yCGr_G

Cuts at:    126     142    1002    1171    1421    1696     126 
   Size:         16     860     169     250     275    3106

  Fragments arranged by size: 

                3106     860     275     250     169      16
 
BamHI G'GATC_C

Cuts at:   1701    1701 
   Size:       4676
 
BanI G'GyrC_C

Cuts at:    469    1692    2106    2354    2389     469 
   Size:       1223     414     248      35    2756

  Fragments arranged by size: 

                2756    1223     414     248      35
 
BanII G_rGCy'C

Cuts at:    151     364    1002    1121    1185    1690    2720     151 
   Size:        213     638     119      64     505    1030    2107

  Fragments arranged by size: 

                2107    1030     638     505     213     119      64
 
BbeI G_GCGC'C

Cuts at:   2110    2358    2110 
   Size:        248    4428
 
BciVI GTATCCnnnnn_n'

Cuts at:   2574    3335    4676    2574 
   Size:        761    1341    2574

  Fragments arranged by size: 

                2574    1341     761
 
BclI T'GATC_A

Cuts at:   2196    2196 
   Size:       4676
 
BglI GCCn_nnn'nGGC

Cuts at:    997     997 
   Size:       4676
 
BglII A'GATC_T

Cuts at:   2191    4228    2191 
   Size:       2037    2639
 
BlpI GC'TnA_GC

Cuts at:    506    1788     506 
   Size:       1282    3394
 
Bme1580I G_kGCm'C

Cuts at:   1002    1185    1422    2301    2394    3450    1002 
   Size:        183     237     879      93    1056    2228

  Fragments arranged by size: 

                2228    1056     879     237     183      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    668    1005    1888    1969    2304    4448     668 
   Size:        337     883      81     335    2144     896

  Fragments arranged by size: 

                2144     896     883     337     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3043    3043 
   Size:       4676
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    231     702    1489    1624     231 
   Size:        471     787     135    3283

  Fragments arranged by size: 

                3283     787     471     135
 
Bpu10I CC'TnA_GC

Cuts at:   1152    1189    1152 
   Size:         37    4639
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    239    1078    1237    1852    2731    3223    3521     239 
   Size:        839     159     615     879     492     298    1394

  Fragments arranged by size: 

                1394     879     839     615     492     298     159
 
BsaI GGTCTCn'nnnn_

Cuts at:    274     424    4380     274 
   Size:        150    3956     570

  Fragments arranged by size: 

                3956     570     150
 
BsaAI yAC'GTr

Cuts at:   1287    1331    2029    1287 
   Size:         44     698    3934

  Fragments arranged by size: 

                3934     698      44
 
BsaBI GATnn'nnATC

Cuts at:      6     769    2190    4227       6 
   Size:        763    1421    2037     455

  Fragments arranged by size: 

                2037    1421     763     455
 
BsaHI Gr'CG_yC

Cuts at:    984    1388    1433    2107    2355    3057    4553     984 
   Size:        404      45     674     248     702    1496    1107

  Fragments arranged by size: 

                1496    1107     702     674     404     248      45
 
BsaWI w'CCGG_w

Cuts at:    236     881    1490    1865    2386    3064    3338    3485    3896 
   Size:        645     609     375     521     678     274     147     411

Cuts at:   3896     236 
   Size:       1016

  Fragments arranged by size: 

                1016     678     645     609     521     411     375     274
                 147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1597    1627    1597 
   Size:         30    4646
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    811    1096    1503     811 
   Size:        285     407    3984

  Fragments arranged by size: 

                3984     407     285
 
BseYI C'CCAG_C

Cuts at:    306     422     449     545     583     834    1122    1638    2101 
   Size:        116      27      96      38     251     288     516     463

Cuts at:   2101    2859    3436    3932     306 
   Size:        758     577     496    1050

  Fragments arranged by size: 

                1050     758     577     516     496     463     288     251
                 116      96      38      27
 
BsiEI CG_ry'CG

Cuts at:    104    1038    1274    2218    2264    3472     104 
   Size:        934     236     944      46    1208    1308

  Fragments arranged by size: 

                1308    1208     944     934     236      46
 
BsiHKAI G_wGCw'C

Cuts at:    247    1121    1690    2468    2658    3450     247 
   Size:        874     569     778     190     792    1473

  Fragments arranged by size: 

                1473     874     792     778     569     190
 
BsmBI CGTCTCn'nnnn_

Cuts at:    965     965 
   Size:       4676
 
Bsp1286I G_dGCh'C

Cuts at:    151     247     364    1002    1121    1185    1422    1690    2301 
   Size:         96     117     638     119      64     237     268     611

Cuts at:   2301    2394    2468    2658    2720    3450     151 
   Size:         93      74     190      62     730    1377

  Fragments arranged by size: 

                1377     730     638     611     268     237     190     119
                 117      96      93      74      64      62
 
BspEI T'CCGG_A

Cuts at:   3896    3896 
   Size:       4676
 
BspHI T'CATG_A

Cuts at:   3852    3852 
   Size:       4676
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1726    1940    2242    2623    1726 
   Size:        214     302     381    3779

  Fragments arranged by size: 

                3779     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    1151    2968    3022    4506    4671      50 
   Size:       1101    1817      54    1484     165      55

  Fragments arranged by size: 

                1817    1484    1101     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   2588    2588 
   Size:       4676
 
BsrFI r'CCGG_y

Cuts at:    888    1108    1490    2674    2855    3064     888 
   Size:        220     382    1184     181     209    2500

  Fragments arranged by size: 

                2500    1184     382     220     209     181
 
BssHII G'CGCG_C

Cuts at:   1477    1477 
   Size:       4676
 
BssSI C'ACGA_G

Cuts at:   1264    2947    3305    1264 
   Size:       1683     358    2635

  Fragments arranged by size: 

                2635    1683     358
 
BstAPI GCAn_nnn'nTGC

Cuts at:   2053    2053 
   Size:       4676
 
BstBI TT'CG_AA

Cuts at:   3037    3037 
   Size:       4676
 
BstYI r'GATC_y

Cuts at:      1     273     686    1607    1701    2168    2191    2526    2772 
   Size:        272     413     921      94     467      23     335     246

Cuts at:   2772    3784    3870    3882    4228       1 
   Size:       1012      86      12     346     449

  Fragments arranged by size: 

                1012     921     467     449     413     346     335     272
                 246      94      86      23      12
 
Bsu36I CC'TnA_GG

Cuts at:   1079    1079 
   Size:       4676
 
BtgI C'CryG_G

Cuts at:    107    1427     107 
   Size:       1320    3356
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2626    2807    2970    2626 
   Size:        181     163    4332

  Fragments arranged by size: 

                4332     181     163
 
BtsI GCAGTG_nn'

Cuts at:    302    1223    1919     302 
   Size:        921     696    3059

  Fragments arranged by size: 

                3059     921     696
 
DraI TTT'AAA

Cuts at:   1675    1675 
   Size:       4676
 
DraIII CAC_nnn'GTG

Cuts at:   1266    1266 
   Size:       4676
 
DrdI GACnn_nn'nnGTC

Cuts at:   2382    3240    2382 
   Size:        858    3818
 
EaeI y'GGCC_r

Cuts at:    860     989    1099    1271    2261    2435    2826    2853    4198 
   Size:        129     110     172     990     174     391      27    1345

Cuts at:   4198     860 
   Size:       1338

  Fragments arranged by size: 

                1345    1338     990     391     174     172     129     110
                  27
 
EagI C'GGCC_G

Cuts at:   1271    2261    1271 
   Size:        990    3686
 
EarI CTCTTCn'nnn_

Cuts at:    422     490    1459    2699    2909     422 
   Size:         68     969    1240     210    2189

  Fragments arranged by size: 

                2189    1240     969     210      68
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    452    1023    2884    3194    3340    4486     452 
   Size:        571    1861     310     146    1146     642

  Fragments arranged by size: 

                1861    1146     642     571     310     146
 
EcoICRI GAG'CTC

Cuts at:   1119    1688    1119 
   Size:        569    4107
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    231     702    1366    1489    1604    1624    2501    2933    3680 
   Size:        471     664     123     115      20     877     432     747

Cuts at:   3680    3948     231 
   Size:        268     959

  Fragments arranged by size: 

                 959     877     747     664     471     432     268     123
                 115      20
 
EcoNI CCTnn'n_nnAGG

Cuts at:    223     223 
   Size:       4676
 
EcoO109I rG'GnC_Cy

Cuts at:    123     214     332     840     999    1156    1181    1182    1569 
   Size:         91     118     508     159     157      25       1     387

Cuts at:   1569    1815     123 
   Size:        246    2984

  Fragments arranged by size: 

                2984     508     387     246     159     157     118      91
                  25       1
 
EcoRI G'AATT_C

Cuts at:   1680    1680 
   Size:       4676
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1038    1263    2695    1038 
   Size:        225    1432    3019

  Fragments arranged by size: 

                3019    1432     225
 
FspI TGC'GCA

Cuts at:   2457    2457 
   Size:       4676
 
HaeII r_GCGC'y

Cuts at:    166    1196    1320    2110    2358    3380    4359     166 
   Size:       1030     124     790     248    1022     979     483

  Fragments arranged by size: 

                1030    1022     979     790     483     248     124
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   2518    2548    2550    2580    2518 
   Size:         30       2      30    4614

  Fragments arranged by size: 

                4614      30      30       2
 
HincII GTy'rAC

Cuts at:    315    1715     315 
   Size:       1400    3276
 
HpaI GTT'AAC

Cuts at:    315     315 
   Size:       4676
 
KasI G'GCGC_C

Cuts at:   2106    2354    2106 
   Size:        248    4428
 
KpnI G_GTAC'C

Cuts at:   1696    1696 
   Size:       4676
 
MluI A'CGCG_T

Cuts at:   4109    4109 
   Size:       4676
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    335    3347    3531     335 
   Size:       3012     184    1480

  Fragments arranged by size: 

                3012    1480     184
 
MscI TGG'CCA

Cuts at:    862    1101    2437     862 
   Size:        239    1336    3101

  Fragments arranged by size: 

                3101    1336     239
 
MslI CAynn'nnrTG

Cuts at:    964    1540    2665    2792     964 
   Size:        576    1125     127    2848

  Fragments arranged by size: 

                2848    1125     576     127
 
MspA1I CmG'CkG

Cuts at:    306     583    1429    1445    1787    2101    2461    3474    3719 
   Size:        277     846      16     342     314     360    1013     245

Cuts at:   3719     306 
   Size:       1263

  Fragments arranged by size: 

                1263    1013     846     360     342     314     277     245
                  16
 
NaeI GCC'GGC

Cuts at:   1110    2857    1110 
   Size:       1747    2929
 
NarI GG'CG_CC

Cuts at:   2107    2355    2107 
   Size:        248    4428
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       4676
 
NgoMIV G'CCGG_C

Cuts at:   1108    2855    1108 
   Size:       1747    2929
 
NspI r_CATG'y

Cuts at:    903    1729    2229    3136     903 
   Size:        826     500     907    2443

  Fragments arranged by size: 

                2443     907     826     500
 
PasI CC'CwG_GG

Cuts at:    154     597    1228     154 
   Size:        443     631    3602

  Fragments arranged by size: 

                3602     631     443
 
PciI A'CATG_T

Cuts at:   3132    3132 
   Size:       4676
 
PflMI CCAn_nnn'nTGG

Cuts at:    680     855     680 
   Size:        175    4501
 
PfoI T'CCnGG_A

Cuts at:   1601    1601 
   Size:       4676
 
PmeI GTTT'AAAC

Cuts at:   1675    1675 
   Size:       4676
 
PmlI CAC'GTG

Cuts at:   1287    1331    1287 
   Size:         44    4632
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1597    1629    3815    3847    1597 
   Size:         32    2186      32    2426

  Fragments arranged by size: 

                2426    2186      32      32
 
PpuMI rG'GwC_Cy

Cuts at:    214     332     840     214 
   Size:        118     508    4050

  Fragments arranged by size: 

                4050     508     118
 
PspOMI G'GGCC_C

Cuts at:    998    1181     998 
   Size:        183    4493
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:   1642    1674    1642 
   Size:         32    4644
 
PstI C_TGCA'G

Cuts at:    917    1723    2408     917 
   Size:        806     685    3185

  Fragments arranged by size: 

                3185     806     685
 
PvuI CG_AT'CG

Cuts at:    104    1038     104 
   Size:        934    3742
 
PvuII CAG'CTG

Cuts at:    306     583    2101    2461     306 
   Size:        277    1518     360    2521

  Fragments arranged by size: 

                2521    1518     360     277
 
RsrII CG'GwC_CG

Cuts at:    234    2871     234 
   Size:       2637    2039
 
SacI G_AGCT'C

Cuts at:   1121    1690    1121 
   Size:        569    4107
 
SacII CC_GC'GG

Cuts at:   1430    1430 
   Size:       4676
 
SalI G'TCGA_C

Cuts at:   1713    1713 
   Size:       4676
 
SanDI GG'GwC_CC

Cuts at:    214     332     840     214 
   Size:        118     508    4050

  Fragments arranged by size: 

                4050     508     118
 
SapI GCTCTTCn'nnn_

Cuts at:   2699    2909    2699 
   Size:        210    4466
 
SbfI CC_TGCA'GG

Cuts at:   1723    1723 
   Size:       4676
 
ScaI AGT'ACT

Cuts at:   4193    4193 
   Size:       4676
 
SfcI C'TryA_G

Cuts at:     33     913    1551    1719    2404    3397    3588      33 
   Size:        880     638     168     685     993     191    1121

  Fragments arranged by size: 

                1121     993     880     685     638     191     168
 
SfiI GGCCn_nnn'nGGCC

Cuts at:    997     997 
   Size:       4676
 
SfoI GGC'GCC

Cuts at:   2108    2356    2108 
   Size:        248    4428
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4676
 
SmaI CCC'GGG

Cuts at:    128    1004    1698     128 
   Size:        876     694    3106

  Fragments arranged by size: 

                3106     876     694
 
SmlI C'TyrA_G

Cuts at:    142     254    1093    1252    1831    2746    3238    3500     142 
   Size:        112     839     159     579     915     492     262    1318

  Fragments arranged by size: 

                1318     915     839     579     492     262     159     112
            
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4676
 
SphI G_CATG'C

Cuts at:   1729    2229    1729 
   Size:        500    4176
 
StyI C'CwwG_G

Cuts at:    107     809    1056    1810    2164     107 
   Size:        702     247     754     354    2619

  Fragments arranged by size: 

                2619     754     702     354     247
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    422    1614    2259    2392    2851    3060     422 
   Size:       1192     645     133     459     209    2038

  Fragments arranged by size: 

                2038    1192     645     459     209     133
 
TatI w'GTAC_w

Cuts at:   1649    4191    1649 
   Size:       2542    2134
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    226     258    4193    4225     226 
   Size:         32    3935      32     677

  Fragments arranged by size: 

                3935     677      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1537    2473    1537 
   Size:        936    3740
 
XbaI T'CTAG_A

Cuts at:   1707    1707 
   Size:       4676
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    114     255    1203    2171     114 
   Size:        141     948     968    2619

  Fragments arranged by size: 

                2619     968     948     141
 
XhoI C'TCGA_G

Cuts at:    142     142 
   Size:       4676
 
XmaI C'CCGG_G

Cuts at:    126    1002    1696     126 
   Size:        876     694    3106

  Fragments arranged by size: 

                3106     876     694
 
XmnI GAAnn'nnTTC

Cuts at:   1021    1561    1021 
   Size:        540    4136
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI     AfeI   AflIII     AgeI     AloI
    AlwNI     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI     AvaI
    BamHI     BanI    BanII     BbeI    BciVI     BclI     BglI    BglII
     BlpI Bme1580I     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI    BsiEI
  BsiHKAI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI   BssHII    BssSI   BstAPI    BstBI    BstYI   Bsu36I     BtgI
    BtgZI     BtsI     DraI   DraIII     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FspI
    HaeII    Hin4I   HincII     HpaI     KasI     KpnI     MluI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
   PspOMI     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI     SfcI     SfiI     SfoI
     SgfI     SmaI     SmlI     SpeI     SphI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII    AflII     AhdI     AleI     AlfI    AvrII     BaeI
     BbsI    BbvCI     BcgI    BfrBI    BmgBI     BmtI     BsgI    BsiWI
     BsmI    BsrGI   BstEII    BstXI  BstZ17I     ClaI    CspCI    EcoRV
     FseI    FspAI  HindIII     MfeI     NdeI     NheI     NotI     NruI
     NsiI     PacI    PshAI     PsiI    PspXI    SexAI    SgrAI    SnaBI
     SrfI     SspI     StuI     SwaI     ZraI
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