Restriction map for pF1KB6734
##################################
# NO CUT ENZYMES (  174 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwI        AlwNI       AocI        Aor51HI     
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspHI       AspI        
AviII       AvrII       BalI        BamHI       BbeI        
BbrPI       BbsI        BbvCI       BcgI        BciVI       
BclI        BfaI        BfrI        BglI        BglII       
BlnI        BlpI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaHI       BsaI        BseAI       BsgI        
BsiHKAI     BsiWI       BsmAI       BsmBI       BsmFI       
BsmI        Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrBI       BsrDI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstYI       BstZ17I     Bsu36I      CelII       Cfr10I      
ClaI        CpoI        DdeI        DraI        DraIII      
DrdI        DsaI        Eam1105I    EarI        Eco47III    
Eco81I      EcoNI       EcoRI       EcoRV       EcoT22I     
FauI        FbaI        FseI        FspI        HaeII       
HgaI        Hin1I       HincII      HindII      HpaI        
KasI        KpnI        Ksp632I     KspI        MaeI        
MaeII       MamI        MfeI        MflI        MluI        
MluNI       MroI        MscI        MseI        MspA1I      
MunI        NaeI        NarI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PleI        PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PstI        PvuI        PvuII       RcaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfaNI       SfcI        SfiI        
SfoI        SfuI        SgrAI       SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        SwaI        
Tru9I       Tsp509I     Tth111I     Van91I      XbaI        
XcmI        XhoI        XhoII       XmnI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AccI          343  GTCTAC               GT^[AC][GT]AC
AccII         270  CGCG                 CG^CG
AfaI          384  GTAC                 GT^AC
ApaI          298  GGGCCC               GGGCC^C
AvaI          295  CCCGGG               C^[CT]CG[GA]G
AvaII          69  GGACC                G^G[AT]CC
BanI          175  GGCACC               G^G[CT][GA]CC
BbvI          233  GCTGC                GCAGC(8/12)
BpmI          220  CTGGAG               CTGGAG(16/14)
BsaWI         332  ACCGGA               [AT]^CCGG[AT]
BsiEI         153  CGGCCG               CG[GA][CT]^CG
BsoBI         295  CCCGGG               C^[CT]CG[GA]G
BspMI         286  ACCTGC               ACCTGC(4/8)
BsrI           18  ACTGG                ACTGG(1/-1)
BstEII        282  GGTCACC              G^GT[ACGT]ACC
BstNI          30  CCTGG                CC^[AT]GG
BstPI         282  GGTCACC              G^GT[ACGT]ACC
BstUI         270  CGCG                 CG^CG
CfoI          196  GCGC                 GCG^C
DpnII          84  GATC                 ^GATC
DraII         298  GGGCCCT              [GA]G^G[ACGT]CC[CT]
EaeI          153  CGGCCG               [CT]^GGCC[GA]
EagI          153  CGGCCG               C^GGCCG
EclXI         153  CGGCCG               C^GGCCG
Eco52I        153  CGGCCG               C^GGCCG
EcoO109I      298  GGGCCCT              [GA]G^G[ACGT]CC[CT]
EcoO65I       282  GGTCACC              G^GT[ACGT]ACC
EcoRII         30  CCTGG                ^CC[AT]GG
EcoT14I       167  CCAAGG               C^C[AT][AT]GG
HhaI          196  GCGC                 GCG^C
HinP1I        196  GCGC                 G^CGC
HindIII       337  AAGCTT               A^AGCTT
HinfI         266  GATTC                G^A[ACGT]TC
MboI           84  GATC                 ^GATC
MboII         102  GAAGA                GAAGA(8/7)
MslI          205  CACTGCTATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI           30  CCTGG                CC^[AT]GG
MvnI          270  CGCG                 CG^CG
NdeII          84  GATC                 ^GATC
RsaI          384  GTAC                 GT^AC
Sau3AI         84  GATC                 ^GATC
SmaI          295  CCCGGG               CCC^GGG
SmlI          352  CTCAAG               C^T[CT][GA]AG
StuI            6  AGGCCT               AGG^CCT
StyI          167  CCAAGG               C^C[AT][AT]GG
TaqI           86  TCGA                 T^CGA
TfiI          266  GATTC                G^A[AT]TC
TseI          233  GCTGC                G^C[AT]GC
TthHB8I        86  TCGA                 T^CGA
XmaCI         295  CCCGGG               C^CCGGG
XmaI          295  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

AluI          338  AGCT                 AG^CT
              370  AGCT                
BanII         292  GAGCCC               G[GA]GC[CT]^C
              298  GGGCCC              
BcnI          295  CCCGG                CC^[GC]GG
              296  CCGGG               
BmyI          292  GAGCCC               G[AGT]GC[ACT]^C
              298  GGGCCC              
BseRI          10  CTCCTC               GAGGAG(10/8)
              223  GAGGAG              
Bsp1286I      292  GAGCCC               G[AGT]GC[ACT]^C
              298  GGGCCC              
BstF5I        402  CATCC                GGATG(2/0)
              171  GGATG               
BstXI          16  CCACTGGCTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              204  CCACTGCTATGG        
FokI          402  CATCC                GGATG(9/13)
              171  GGATG               
HapII         296  CCGG                 C^CGG
              333  CCGG                
HpaII         296  CCGG                 C^CGG
              333  CCGG                
HphI           93  GGTGA                GGTGA(8/7)
              284  TCACC               
MspI          296  CCGG                 C^CGG
              333  CCGG                
NciI          295  CCCGG                CC^[GC]GG
              296  CCGGG               
NlaIII        199  CATG                 CATG^
              310  CATG                
TspRI          17  CACTG                CA[GC]TG[ACGT][ACGT]^
              205  CACTG               


#########################################
# 3 OR MORE CUT ENZYMES (   17 enzymes) #
#########################################

AciI        BsaJI       BsiYI       BslI        BssKI       
Cac8I       Cfr13I      Fnu4HI      HaeIII      ItaI        
MaeIII      MnlI        MwoI        NlaIV       Sau96I      
ScrFI       Tsp45I      
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