Restriction map for pF1KB6768
##################################
# NO CUT ENZYMES (  189 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwNI       AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspHI       AspI        
AvaII       AviII       AvrII       BalI        BamHI       
BbeI        BbrPI       BbsI        BbvCI       BcgI        
BciVI       BfrI        BglI        BglII       BlnI        
BlpI        BpmI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaHI       BsaI        BsaWI       BseAI       
BseRI       BsgI        BsiHKAI     BsiWI       BsmAI       
BsmBI       BsmFI       BsmI        Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrDI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstUI       BstYI       BstZ17I     Bsu36I      CelII       
CfoI        Cfr10I      ClaI        CpoI        DraI        
DraII       DraIII      DrdI        EagI        Eam1105I    
EarI        EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO109I    EcoO65I     EcoRI       EcoRV       
EcoT14I     EcoT22I     FauI        FseI        FspI        
HaeII       HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HindIII     HinfI       HpaI        
KasI        KpnI        Ksp632I     KspI        MaeII       
MamI        MboII       MfeI        MflI        MluI        
MluNI       MroI        MscI        MseI        MunI        
MvnI        NaeI        NarI        NcoI        NdeI        
NgoMIV      NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        RcaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StyI        SwaI        TaqI        TfiI        
Tru9I       Tth111I     TthHB8I     Van91I      XbaI        
XcmI        XhoI        XhoII       XmnI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AcsI          250  AAATTT               [GA]^AATT[CT]
AfaI          270  GTAC                 GT^AC
AlwI          102  GATCC                GGATC(4/5)
ApoI          250  AAATTT               [GA]^AATT[CT]
BanI          169  GGCACC               G^G[CT][GA]CC
BanII         162  GGGCTC               G[GA]GC[CT]^C
BclI           89  TGATCA               T^GATCA
BfaI           16  CTAG                 C^TAG
BmyI          162  GGGCTC               G[AGT]GC[ACT]^C
BsiEI         360  CGACCG               CG[GA][CT]^CG
BsiYI         117  CCCCTTCTTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          117  CCCCTTCTTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1286I      162  GGGCTC               G[AGT]GC[ACT]^C
BsrI          186  ACTGG                ACTGG(1/-1)
BstF5I        180  GGATG                GGATG(2/0)
BstXI         230  CCAAGAAGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I          15  GCTAGC               GC[ACGT]^[ACGT]GC
Cfr13I         65  GGGCC                G^G[ACGT]CC
DdeI          165  CTCAG                C^T[ACGT]AG
DsaI          176  CCGTGG               C^C[GA][CT]GG
EaeI           35  TGGCCG               [CT]^GGCC[GA]
FbaI           89  TGATCA               T^GATCA
FokI          180  GGATG                GGATG(9/13)
HapII         106  CCGG                 C^CGG
HpaII         106  CCGG                 C^CGG
HphI          311  TCACC                GGTGA(8/7)
MaeI           16  CTAG                 C^TAG
MaeIII        113  GTGAC                ^GT[ACGT]AC
MspI          106  CCGG                 C^CGG
NheI           15  GCTAGC               G^CTAGC
NlaIV         169  GGCACC               GG[ACGT]^[ACGT]CC
PvuII         243  CAGCTG               CAG^CTG
RsaI          270  GTAC                 GT^AC
Sau96I         65  GGGCC                G^G[ACGT]CC
SfaNI          53  GCATC                GCATC(5/9)
SfcI          315  CTGTAG               C^T[GA][CT]AG
SmaI          105  CCCGGG               CCC^GGG
StuI          341  AGGCCT               AGG^CCT
Tsp45I        113  GTGAC                ^GT[GC]AC
Tsp509I       251  AATT                 ^AATT
TspRI          32  CAGTG                CA[GC]TG[ACGT][ACGT]^
XmaCI         105  CCCGGG               C^CCGGG
XmaI          105  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

AvaI           62  CTCGGG               C^[CT]CG[GA]G
              105  CCCGGG              
BcnI          105  CCCGG                CC^[GC]GG
              106  CCGGG               
BsoBI          62  CTCGGG               C^[CT]CG[GA]G
              105  CCCGGG              
BstNI         225  CCAGG                CC^[AT]GG
              417  CCTGG               
DpnII          90  GATC                 ^GATC
              102  GATC                
EcoRII        225  CCAGG                ^CC[AT]GG
              417  CCTGG               
MboI           90  GATC                 ^GATC
              102  GATC                
MnlI           61  CCTC                 CCTC(7/6)
              340  GAGG                
MslI          171  CACCCCCGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              287  CATACTCATG          
MspA1I         48  CAGCGG               C[AC]G^C[GT]G
              243  CAGCTG              
MvaI          225  CCAGG                CC^[AT]GG
              417  CCTGG               
NciI          105  CCCGG                CC^[GC]GG
              106  CCGGG               
NdeII          90  GATC                 ^GATC
              102  GATC                
NlaIII        208  CATG                 CATG^
              293  CATG                
Sau3AI         90  GATC                 ^GATC
              102  GATC                


#########################################
# 3 OR MORE CUT ENZYMES (   11 enzymes) #
#########################################

AciI        AluI        BbvI        BsaJI       BssKI       
Fnu4HI      HaeIII      ItaI        MwoI        ScrFI       
TseI        
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